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The Subglacial Antarctic Lakes Scientific Access (SALSA) Project accessed Mercer Subglacial Lake using environmentally clean hot-water drilling to examine interactions among ice, water, sediment, rock, microbes and carbon reservoirs within the lake water column and underlying sediments. A ~0.4 m diameter borehole was melted through 1087 m of ice and maintained over ~10 days, allowing observation of ice properties and collection of water and sediment with various tools. Over this period, SALSA collected: 60 L of lake water and 10 L of deep borehole water; microbes >0.2 μm in diameter from in situ filtration of ~100 L of lake water; 10 multicores 0.32–0.49 m long; 1.0 and 1.76 m long gravity cores; three conductivity–temperature–depth profiles of borehole and lake water; five discrete depth current meter measurements in the lake and images of ice, the lake water–ice interface and lake sediments. Temperature and conductivity data showed the hydrodynamic character of water mixing between the borehole and lake after entry. Models simulating melting of the ~6 m thick basal accreted ice layer imply that debris fall-out through the ~15 m water column to the lake sediments from borehole melting had little effect on the stratigraphy of surficial sediment cores.

Subglacial Antarctic aquatic environments are important targets for scientific exploration due to the unique ecosystems they support and their sediments containing palaeoenvironmental records. Directly accessing these environments while preventing forward contamination and demonstrating that it has not been introduced is logistically challenging. The Whillans Ice Stream Subglacial Access Research Drilling (WISSARD) project designed, tested and implemented a microbiologically and chemically clean method of hot-water drilling that was subsequently used to access subglacial aquatic environments. We report microbiological and biogeochemical data collected from the drilling system and underlying water columns during sub-ice explorations beneath the McMurdo and Ross ice shelves and Whillans Ice Stream. Our method reduced microbial concentrations in the drill water to values three orders of magnitude lower than those observed in Whillans Subglacial Lake. Furthermore, the water chemistry and composition of microorganisms in the drill water were distinct from those in the subglacial water cavities. The submicron filtration and ultraviolet irradiation of the water provided drilling conditions that satisfied environmental recommendations made for such activities by national and international committees. Our approach to minimizing forward chemical and microbiological contamination serves as a prototype for future efforts to access subglacial aquatic environments beneath glaciers and ice sheets.

Mars Polar Science is an integrated, compelling system that serves as a nearby analogue to numerous other planets, supports human exploration, and habitability. Mars possesses the closest and most easily accessible layered ice deposits outside of Earth, and accessing those layers to read the climate record would be a triumph for planetary science.

E. Gaidos et al, 2020, Après Nous, le Déluge: A Human‐Triggered Jökulhlaup From a Subglacial Lake, Geophysical Research Letters, 47, Citation number, 10.1029/2020GL089876. To view the published open abstract, go to https://doi.org/10.1029/2020GL089876. Deposited by shareyourpaper.org and openaccessbutton.org. We've taken reasonable steps to ensure this content doesn't violate copyright. However, if you think it does you can request a takedown by emailing help@openaccessbutton.org.

Wet-based regions of glaciers and ice sheets are now recognized to host unique and diverse microbial communities capable of influencing global biogeochemical cycles. However, the isolated nature of subglacial environments poses limitations upon the supply of protons for chemical weathering and energy sources (electron donors/acceptors) to support in situ microbial communities. A less well recognized source of these substrates is the release of gases from mineral structures, pore spaces or fluid inclusions and the generation of gases from the breakage of mineral bonds during the mechanical breakdown of rocks by moving ice. Here, we investigate the potential release of H2, CO2, CO, and short chain hydrocarbons, particularly CH4, by glacial erosion at rates relevant to chemical weathering and microbial activity beneath glaciers. A wide range of magmatic, metamorphic, and sedimentary rocks, and subglacial sediments from glaciated catchments in Greenland, Norway and Canada were ground in the laboratory to varying grain sizes and the release of gases was measured. The volume of gas released increased as the grain size of the ground sediments decreased. The results of these laboratory experiments were used to estimate rates of catchment-scale gas release based upon estimates of long term abrasion rates at each glacier. H2 generation was calculated to be sufficient to potentially support previously estimated rates of methanogenesis in the upper centimeters of subglacial sediment at a gneissic catchment in Greenland and a sedimentary catchment in Canada. Sufficient CO2 could be released by grinding to drive as much as 20% of subglacial chemical weathering at a metamorphic catchment in Svalbard, with potential implications for the inferred quantity of CO2 drawn-down from the atmosphere by glacial weathering. Rates of CH4 generation from grinding bedrock has the potential to be greater than subglacial microbial generation in a sedimentary catchment in Canada with carbon rich bedrock, suggesting a potentially important source of CH4 for methanotrophic microorganisms. We conclude that mechanical erosion beneath a range of glaciers generates significant quantities of gases which have the potential to enhance chemical weathering and/or support subglacial microbial communities in the deep icy biosphere.

A possible analog for saline lakes on planetary ice bodies lurks beneath a Canadian Arctic ice cap., Subglacial lakes are unique environments that, despite the extreme dark and cold conditions, have been shown to host microbial life. Many subglacial lakes have been discovered beneath the ice sheets of Antarctica and Greenland, but no spatially isolated water body has been documented as hypersaline. We use radio-echo sounding measurements to identify two subglacial lakes situated in bedrock troughs near the ice divide of Devon Ice Cap, Canadian Arctic. Modeled basal ice temperatures in the lake area are no higher than −10.5°C, suggesting that these lakes consist of hypersaline water. This implication of hypersalinity is in agreement with the surrounding geology, which indicates that the subglacial lakes are situated within an evaporite-rich sediment unit containing a bedded salt sequence, which likely act as the solute source for the brine. Our results reveal the first evidence for subglacial lakes in the Canadian Arctic and the first hypersaline subglacial lakes reported to date. We conclude that these previously unknown hypersaline subglacial lakes may represent significant and largely isolated microbial habitats, and are compelling analogs for potential ice-covered brine lakes and lenses on planetary bodies across the solar system.

Ice shelves surround ∼ 75% of Antarctica's coastline and are highly sensitive to climate change; several have recently collapsed and others are predicted to in the near future. Marine waters beneath ice shelves harbor active ecosystems, while adjacent seas can be important areas of bottom water formation. Despite their oceanographic significance, logistical constraints have resulted in few opportunities to directly sample sub-ice shelf cavities. Here, we present the first data on microbial diversity and biogeochemistry beneath the McMurdo Ice Shelf (MIS) near Ross Island, Antarctica. Physicochemical profiles obtained via a 56 m deep borehole through the MIS revealed three vertically layered water masses (Antarctic Surface Water [AASW], Ice Shelf Water [ISW], and modified High Salinity Shelf Water [mHSSW]). Metabolically active, moderately diverse (Shannon diversity from 2.06 to 5.74) microbial communities were detected in the AASW and mHSSW. Heterotrophic bacterial production and dissolved organic matter concentrations were higher (12–37% and 24%, respectively) in mHSSW relative to AASW. Chemoautotrophic production was 5.3 nmol C L−1 d−1 and 6.0 nmol C L−1 d−1 in the AASW and mHSSW, respectively. Phytoplankton cells were more abundant and larger in the mHSSW sample relative to the AASW, which indicates sinking of phytoplankton produced in surface waters and, together with southerly flowing currents (0.09–0.16 m s−1), horizontal advection of phytoplankton from McMurdo Sound. Advected phytoplankton carbon together with in situ chemoautotrophic production provide important sources of organic matter and other reduced compounds to support ecosystem processes in the dark waters in the ice shelf cavity.

Due to solute impurities and freezing-point depression in polycrystalline ice, a complicated and dynamic network of liquid water forms within the solid ice matrix at the boundaries between ice crystal grains. Impurity concentrations, temperature and pressure influence this network structure and impact physical, transport and rheological properties of ice. However, the nature of this internal network structure is not fully understood. Here we utilize nuclear magnetic resonance (NMR) measurements of diffusion and magnetic relaxation to study the geometry and interconnectivity of the liquid-filled network in laboratory ice, formed from a 7 g L−1 NaCl solution, and its evolution due to recrystallization processes. Additionally, we apply these NMR measurements to observe the impact on ice microstructure of an ice-binding protein (IBP) excreted by the V3519-10 organism (Flavobacteriaceae family) isolated from the Vostok ice core in Antarctica. Recrystallization inhibition was observed as a function of IBP concentration. This work demonstrates the utility of advanced NMR techniques for applications to ice microstructure and has broader implications for understanding geophysical properties of cryospheric systems.

The oldest evidence of eukaryotes in the fossil record comes from a recurrent assemblage of morphologically differentiated late Paleoproterozoic to early Mesoproterozoic microfossils. Although widely distributed, the principal constituents of this Tappania-Dictyosphaera-Valeria assemblage have not hitherto been recognized on Laurentia. We have recovered all three taxa from a shallow-water shale succession in the early Mesoproterozoic Greyson Formation (Belt Supergroup, Montana, USA). An exceptionally preserved population of Tappania substantially expands the morphological range of this developmentally complex organism, suggesting phylogenetic placement within, or immediately adjacent to, crown-group eukaryotes. Correspondence with Tappania-bearing biotas from China, India, Australia, and Siberia demonstrates an open-ocean connection to the intracratonic Belt Basin and, along with broadly co-occurring macrofossils Grypania and Horodyskia, supports the recognition of a globally expressed biozone. The Greyson Formation, along with contiguous strata in Glacier National Park, is unique in preserving all currently confirmed taxa of early eukaryotic and macroscopic fossils.

Sandstone and carbonate core samples were challenged with a two-phase supercritical CO2 and brine mixture to investigate the effects of chemical processes on the physical properties of these rocks during injection of CO2. The experiments were monitored in real-time for pressure, temperature, and volumetric rate discharge. Pore geometry and connectivity were characterized before and after each experimental challenge using magnetic resonance (MR) imaging and two-dimensional MR relaxation correlations. Quartz arenite sandstone cores were largely unaffected by the challenge with no measurable change in effective permeability at moderate and high temperatures (~50 °C and ~95 °C) or brine concentrations (~1 g/L and ~10 g/L). In contrast, a carbonate core sample showed evidence of significant dissolution leading to a six-fold increase in effective permeability. MR images and relaxation measurements revealed a marked increase in the volume and connectivity of pre-existing pore networks in the carbonate core. We infer that the increase in permeability in the carbonate core was enhanced by focused dissolution in the existing pore and fracture networks that enhanced fast-flow paths through the core.

Rock glaciers are periglacial alpine landforms that are found in many locations worldwide. Whereas well-developed models of deformation are established for traditional alpine glaciers, rock glacier deformation is poorly understood. Geophysical data from Lone Peak Rock Glacier (LPRG), southwest Montana, USA, are paired with lidar bare-earth 1 m digital elevation model (DEM) analysis to explore potential genetic relationships between internal composition, structure and regularly spaced arcuate transverse ridges expressed at the rock glacier surface. The internal composition of LPRG is heterogeneous, with frozen debris and clean ice overlain by an unconsolidated talus mantle. Upslope-dipping, clearly distinguished reflectors in the ground-penetrating radar (GPR) longitudinal survey at LPRG correspond to transverse ridges. The spacing and slope of individual features at the surface and in the subsurface were measured and compared and are found to be similar. The structures observed at LPRG and other rock glaciers are similar to structures detected in glaciotectonically altered sediment, ice-cored moraines and other rock glacier settings. This finding suggests that transverse ridges on rock glaciers may be used as geomorphic indicators of internal deformation. This study contributes to the body of research on the application of GPR to rock glaciers, and is the first to directly pair and analyze individual surface topographic features with internal structures.

Two ∼4 m vertical sequences of basal ice were collected from tunnels dug into the northern lateral margin of Taylor Glacier, McMurdo Dry Valleys, Antarctica. In both cases the basal sequences exhibit two contrasting ice facies groups; clean (debris-free) and banded dispersed (debris-rich). Debris-rich ices exhibit elevated CO2 and depleted O2 concentrations compared to the clean facies. Bacterial cell numbers, respiration rates, and nutrient concentrations are highest in debris-rich layers. Together, our geochemical and biological data indicate that microbial heterotrophic respiration is likely occurring in situ within the basal ice matrix at ambient temperatures near −15°C. This implies that the basal ice zone of polar glaciers and larger ice sheets is a viable subglacial microbial habitat and active biome of significant volume that has not previously been considered.

Microorganisms in subglacial environments drive the chemical weathering of bedrock; however, the influence of bedrock mineralogy on the composition and activity of microbial assemblages in such environments is poorly understood. Here, using a combination of in situ mineral incubation and DNA fingerprinting techniques, we demonstrate that pyrite is the dominant mineralogical control on subglacial bacterial community structure and composition. In addition, we show that the abundance of Fe in the incubated minerals influences the development of mineral-associated biomass. The ubiquitous nature of pyrite in many common bedrock types and high SO 4 2– concentrations in most glacial meltwaters suggest that pyrite may be a dominant lithogenic control on microbial communities in many subglacial systems. Mineral-based energy may therefore serve a fundamental role in sustaining subglacial microbial populations and enabling their persistence over glacial-interglacial time scales.

The Whillans Ice Stream Subglacial Access Research Drilling (WISSARD) project will test the overarching hypothesis that an active hydrological system exists beneath a West Antarctic ice stream that exerts a major control on ice dynamics, and the metabolic and phylogenetic diversity of the microbial community in subglacial water and sediment. WISSARD will explore Subglacial Lake Whillans (SLW, unofficial name) and its outflow toward the grounding line where it is thought to enter the Ross Ice Shelf seawater cavity. Introducing microbial contamination to the subglacial environment during drilling operations could compromise environmental stewardship and the science objectives of the project, consequently we developed a set of tools and procedures to directly address these issues. WISSARD hot water drilling efforts will include a custom water treatment system designed to remove micron and sub-micron sized particles (biotic and abiotic), irradiate the drilling water with germicidal ultraviolet (UV) radiation, and pasteurize the water to reduce the viability of persisting microbial contamination. Our clean access protocols also include methods to reduce microbial contamination on the surfaces of cables/hoses and down-borehole equipment using germicidal UV exposure and chemical disinfection. This paper presents experimental data showing that our protocols will meet expectations established by international agreement between participating Antarctic nations.

Microbes live under glaciers that have persisted for millions of years, without a clear energy supply. Analyses of crushed rocks suggest that interactions of glaciers with the rocks beneath can produce enough H2 to support methanogenic bacteria. Substantial parts of the beds of glaciers, ice sheets and ice caps are at the pressure melting point1. The resulting water harbours diverse subglacial microbial ecosystems2,3 capable of affecting global biogeochemical cycles4,5. Such subglacial habitats may have acted as refugia during Neoproterozoic glaciations6. However, it is unclear how life in subglacial environments could be supported during glaciations lasting millions of years because energy from overridden organic carbon would become increasingly depleted7,8. Here we investigate the potential for abiogenic H2 produced during rock comminution to provide a continual source of energy to support subglacial life. We collected a range of silicate rocks representative of subglacial environments in Greenland, Canada, Norway and Antarctica and crushed them with a sledgehammer and ball mill to varying surface areas. Under an inert atmosphere in the laboratory, we added water, and measured H2 production with time. H2 was produced at 0 °C in all silicate–water experiments, probably through the reaction of water with mineral surface silica radicals formed during rock comminution. H2 production increased with increasing temperature or decreasing silicate rock grain size. Sufficient H2 was produced to support previously measured rates of methanogenesis under a Greenland glacier. We conclude that abiogenic H2 generation from glacial bedrock comminution could have supported life and biodiversity in subglacial refugia during past extended global glaciations.

Laboratory experiments on reagent-grade calcium carbonate and carbonate rich glacial sediments demonstrate previously unreported kinetic fractionation of carbon isotopes during the initial hydrolysis and early stages of carbonate dissolution driven by atmospheric CO2. There is preferential dissolution of Ca 12 CO3 during hydrolysis, resulting in 13 C-DIC values that are significantly lighter isotopically than the bulk carbonate. The fractionation factor for this kinetic isotopic effect is defined as carb. carb is greater on average for glacial sediments (17.4‰) than for calcium carbonate (7.8‰) for the 63 m size fraction, a sediment concentration o f5gL 1 and closed system conditions at 5°C. This difference is most likely due to the preferential dissolution of highly reactive ultra-fine particles with damaged surfaces that are common in subglacial sediments. The kinetic isotopic fractionation has a greater impact on 13 C-DIC at higher CaCO3: water ratios and is significant during at least the firs t6ho fcarbonate dissolution driven by atmospheric CO2 at sediment concentrations o f5gL 1 . Atmospheric CO2 dissolving into solution following carbonate hydrolysis does not exhibit any significant equilibrium isotopic fractionation for at least 6 h after the start of the experiment at 5°C. This is considerably longer than previously reported in the literature. Thus, kinetic fractionation processes will likely dominate the 13 C-DIC signal in natural environments where rock:water contact times are short 6 -24 h (e.g., glacial systems, headwaters in fluvial catchments) and there is an excess of carbonate in the sediments. It will be difficult apply conventional isotope mass balance techniques in these types of environment to identify microbial CO2 signatures in DIC from 13 C-DIC data. Copyright © 2004

The relationship between subglacial chemical weathering processes and the Sr isotope composition of runoff from Robertson Glacier, Alberta, Canada, is investigated. This glacier rests on predominantly carbonate bedrock of Upper Devonian age, but silicate minerals are also present. The provenance of solute in meltwaters is found to vary systematically with solute concentration and, by inference, subglacial water residence time. In dilute waters, the principal process of solute acquisition is calcite dissolution fueled by protons derived from the dissolution of CO 2 and subsequent dissociation of carbonic acid. At higher solute concentrations, dolomite dissolution coupled to sulfide oxidation is more important. Sr concentration is found to increase with total solute concentration in two separate meltwater streams draining from the glacier, but 87 Sr/ 86 Sr only increases in the eastern melt stream. Carbonate and K-feldspar sources are shown to dominate the Sr content of the western stream, irrespective of concentration. They also dominate the Sr content of the eastern stream at low and intermediate concentrations, but at higher concentrations, muscovite (with high 87 Sr/ 86 Sr) is also an important Sr source. This reflects the outcrop of muscovite-bearing lithologies in the catchment of the eastern stream and an increase in the rate of weathering of K-silicates relative to that of carbonates as more concentrated solutions approach saturation with respect to carbonates. Nonstoichiometric release of 87 Sr/ 86 Sr and preferential release of Sr over K from freshly ground K-silicate surfaces may also occur. This may help to explain the radiogenic nature of runoff from distributed subglacial drainage systems, which are characterized by long water:rock contact times and water flow through environments in which crushing and grinding of bedrock are active processes. Although the exchangeable Sr in tills has higher 87 Sr/ 86 Sr than local carbonate bedrock, only the more concentrated meltwaters from the eastern stream display similarly high values. The most dilute waters, which probably transport the bulk of the dissolved Sr flux from the glacier, have 87 Sr/ 86 Sr characteristic of local carbonate bedrock. Thus, the results suggest that although enhanced weathering of silicate minerals containing radiogenic Sr (such as muscovite) does occur in glaciated carbonate terrains, it is unlikely to contribute to any enhanced flux of radiogenic Sr from glaciated continental surfaces to the oceans.

This paper describes the physical and chemical properties of the snow- pack on John Evans Glacier, Ellesmere Island, Canadian Arctic Archipelago, and investigates the controls on snowpack solute concentrations and atmospheric deposition. The snowpack contains three layers that are traceable across the whole glacier. These represent fall accumulation that has been metamorphosed to depth hoar, winter accumulation mixed with snow reworked by wind from the underlying depth hoar, and spring accumulation mixed with wind-reworked snow. The seasonal cycle in snow chemistry closely reflects changes in the composition of the atmospheric aerosol at Alert, with some modification of NO3− concentrations by post-depositional processes. Mean water-weighted solute concentrations in the snowpack are largely independent of accumulation, while atmospheric deposition tends to increase with accumulation. This suggests that, for most species, wet deposition is the dominant depositional process throughout the year. However, concentrations of Ca2+ and K+ increase with both accumulation and elevation, implying an enhanced input from dry deposition of soil dust above 800 m elevation. Concentrations of SO42− are inversely related to accumulation, especially in the winter layer, suggesting a significant input from non-precipitating events, such as dry deposition or riming, during this period of very limited snowfall.

This chapter focuses on glaciers and ice sheets, sea ice, and ice shelves of the polar regions, i.e., those latitudes above the Arctic and Antarctic Circles where glaciers and ice sheets cover a significant proportion of the land mass and where large expanses of the surface waters of the Arctic and Southern Oceans undergo an annual cycle of freezing and melting. This chapter further introduces sea ice as a microbial habitat and summarizes from some of the aforementioned reviews what is known to date about the abundance, activity, diversity, and ecology of prokaryotic sea-ice microorganisms. It provides a brief outline of the role of microorganisms in biogeochemical cycling of elements in sea ice. The majority of bacteria isolated from sea ice are pigmented and highly cold adapted, with some able to form gas vesicles. Possible cold-adaptation strategies revealed by whole-genome sequence analysis also include the production of cryoprotective osmolytes and exopolymers. Polar ice shelves are thick masses of ice floating on the ocean. They are formed through glacial ice and ice sheets pushing onto the sea or long-term accumulations of sea ice. Analysis of ice-shelf heterotrophic bacteria and microbial eukaryotes suggests phylogenetic affiliation with taxa from diverse environments and climatic zones ranging from Antarctica and other cryosphere habitats to temperate ecozones. Microbial investigations on polar glaciers, ice sheets, and ice shelves are still largely in their infancy, with sea-ice research being somewhat more established.

Measurements made at John Evans Glacier, eastern Ellesmere Island, Nunavut, Canada in 1994 and 1996 provide new insight into the internal hydrology of polythermal glaciers. During the early part of each melt season, supraglacial waters enter the glacier via a crevasse field about 4 km from the terminus and are stored in a subglacial reservoir. Release of the water from the reservoir occurs initially via an artesian fountain on the glacier surface and by upwelling of waters through subglacial sediments at the terminus (event1). Channelization of the subglacial waters then occurs and water is discharged as an outburst flood (event 2), which releases a considerably larger volume of water than event 1. Thus, drainage of the subglacial reservoir follows a cyclical pattern in which discharge oscillations increase in amplitude over time. The cycle may end with complete reservoir drainage. The total volume of water released was much greater in 1994 than in 1996, primarily because the 1994 melt season was longer and warmer than the 1996 season. Interannual differences in the form of the outflow hydrographs, and in the extent and timing of connections between the subglacial reservoir and marginal melt streams, are linked to variations in the size and rate of growth of the subglacial reservoir. This hydrological behaviour may have important implications for the dynamics of polythermal glaciers.

Liquid water has been known to occur beneath the Antarctic ice sheet for more than 40 years, but only recently have these subglacial aqueous environments been recognized as microbial ecosystems that may influence biogeochemical transformations on a global scale. Here we present the first geomicrobiological description of water and surficial sediments obtained from direct sampling of a subglacial Antarctic lake. Subglacial Lake Whillans (SLW) lies beneath approximately 800 m of ice on the lower portion of the Whillans Ice Stream (WIS) in West Antarctica and is part of an extensive and evolving subglacial drainage network. The water column of SLW contained metabolically active microorganisms and was derived primarily from glacial ice melt with solute sources from lithogenic weathering and a minor seawater component. Heterotrophic and autotrophic production data together with small subunit ribosomal RNA gene sequencing and biogeochemical data indicate that SLW is a chemosynthetically driven ecosystem inhabited by a diverse assemblage of bacteria and archaea. Our results confirm that aquatic environments beneath the Antarctic ice sheet support viable microbial ecosystems, corroborating previous reports suggesting that they contain globally relevant pools of carbon and microbes that can mobilize elements from the lithosphere and influence Southern Ocean geochemical and biological systems.

[1] The basal regions of continental ice sheets are gaps in our current understanding of the Earth’s biosphere and biogeochemical cycles. We draw on existing and new chemical data sets for subglacial meltwaters to provide the first comprehensive assessment of sub‐ice sheet biogeochemical weathering. We show that size of the ice mass is a critical control on the balance of chemical weathering processes and that microbial activity is ubiquitous in driving dissolution. Carbonate dissolution fueled by sulfide oxidation and microbial CO2 dominate beneath small valley glaciers. Prolonged meltwater residence times and greater isolation characteristic of ice sheets lead to the development of anoxia and enhanced silicate dissolution due to calcite saturation. We show that sub‐ice sheet environments are highly geochemically reactive and should be considered in regional and global solute budgets. For example, calculated solute fluxes from Antarctica (72–130 t yr −1 ) are the same order of magnitude as those from some of the world’s largest rivers and rates of chemical weathering (10–17 t km −2 yr −1 ) are high for the annual specific discharge (2.3–4.1 × 10 −3 m). Our model of chemical weathering dynamics provides important information on subglacial biodiversity and global biogeochemical cycles and may be used to design strategies for the first sampling of Antarctic Subglacial Lakes and other sub‐ice sheet environments for the next decade.

Recently, new data have been acquired on the chemical composition of waters within the glacial till beneath the Kamb Ice Stream (KIS, formerly Ice Stream C) and the Bindschadler Ice Stream (BIS, formerly Ice Stream D), two of the arteries of ice loss from the Antarctic Ice Sheet (AIS). We compare this water chemistry with basally stored subglacial waters from Arctic and Alpine glaciers and the only other measurements of sub-ice sheet chemistry to date, that emerging from beneath Law Dome near Casey Station, SW Antarctica, during a jokulhlaup. We find that the chemistries pose significant questions about the hydrology and biogeochemistry of ice sheet beds. Copyright © 2010 John Wiley & Sons, Ltd.

[1] Glacial meltwaters draining shield bedrock under the Greenland Ice Sheet (GIS) contain

Nature Geoscience 8, 851–855 (2015); published online 29 October 2015; corrected after print 29 October 2015. In the print version of this Letter originally published, the published online date was incorrectly stated as 21 September 2015; the correct published online date is 29 October 2015. This has been corrected in all online versions of the Letter.

We describe a viable microbial community in a subglacial lake within the Grímsvötn volcanic caldera, Iceland. We used a hot water drill to penetrate the 300-m ice shelf and retrieved lake water and volcanic tephra sediments. We also acquired samples of borehole water before and after penetration to the lake, overlying glacial ice and snow, and water from a nearby subaerial geothermal lake for comparative analyses. Lake water is at the freezing point and fresh (total dissolved solids = 260 mg L(-1)). Detectable numbers of cells were found in samples of the lake water column and tephra sediments: 2 x 10(4) ml(-1) and 4 x 10(7) g(-1), respectively. Plate counts document abundant cold-adapted cultivable organisms in the lake water, but not in the borehole (before penetration) or glacial ice. Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments amplified from genomic DNA extracted from Grímsvötn samples indicates that the lake community is distinct from the assemblages of organisms in borehole water (before penetration) and the overlying ice and snow. Sequencing of selected DGGE bands revealed that many sequences are highly similar to known psychrophilic organisms or cloned DNA from other cold environments. Significant uptake of 14C-labeled bicarbonate occurred in dark, low-temperature incubations of lake water samples, indicating the presence of autotrophs. Acetylene reduction assays under similar incubation conditions showed no significant nitrogen fixation potential by lake water samples. This may be a consequence of the inhibition of diazotrophy by nitrogen in the lake.

Solute fluxes from a glacier-covered alpine catchment are partitioned into components derived from sea-salt, acid aerosol, dissolution of atmospheric CO2, and crustal weathering. The bulk of solute is crustally derived. Coupled sulfide oxidation and carbonate dissolution (SO-CD) and carbonation of carbonate minerals generate approximately equal amounts of solute. Chemical denudation constitutes