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3 results on '"Skea DL"'

1. Acquired resistance to Giardia muris in X-linked immunodeficient mice.

Author

Skea DL and Underdown BJ

Subjects
Animals, Genetic Linkage, Immunoglobulin A analysis, Immunologic Deficiency Syndromes genetics, Immunotherapy, Adoptive, Male, Mice, Mice, Inbred CBA, Spleen immunology, X Chromosome, Giardiasis immunology, Immunologic Deficiency Syndromes immunology
Abstract

A previous study from this laboratory (D. P. Snider, D. Skea, and B. J. Underdown, Infect. Immun. 56:2838-2842, 1988) indicated that immunodeficient mice expressing the xid gene develop prolonged infections with Giardia muris, unlike immunocompetent mice, which eliminate the intestinal protozoan parasite in 8 to 10 weeks. In this study, CBA/N (xid) and CBA/Ca mice were infected with G. muris cysts and at various times following this primary infection were cured by treatment with metronidazole. In contrast to the marked differences in the ability of xid and normal mice to eliminate a primary infection, mice of both strains were resistant to a secondary challenge of G. muris cysts. These data imply that the mechanism(s) responsible for elimination of a primary infection is not identical to those required to resist a secondary challenge infection. Splenocytes from immunocompetent CBA/Ca mice (but not immunodeficient CBA/N mice) could transfer the ability to eliminate a primary G. muris infection to irradiated mice of either strain. In contrast, splenocytes from previously infected CBA/Ca mice could not transfer resistance to a challenge infection, further supporting the hypothesis that there are differences between mechanisms required to eliminate a primary infection and those necessary to resist a second challenge infection.

Published
1991
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3. Chronic giardiasis in B-cell-deficient mice expressing the xid gene

Author

Denis P. Snider, Skea Dl, and B J Underdown

Subjects
Immunoglobulin A, Giardiasis, Male, Ratón, Immunology, Congenic, Antibodies, Protozoan, chemical and pharmacologic phenomena, Immunoglobulin E, Microbiology, Mice, Antigen, Immunity, medicine, Animals, B cell, B-Lymphocytes, biology, Immunologic Deficiency Syndromes, Mice, Mutant Strains, Infectious Diseases, medicine.anatomical_structure, Chronic Disease, biology.protein, Parasitology, Female, Antibody, Research Article
Abstract

The role of antibody in immunity to Giardia muris infection was investigated by studying B-cell-deficient CBA/N mice expressing the xid gene. After gastric administration of infective G. muris cysts, CBA/N male and female mice developed prolonged G. muris infection, whereas BALB/c mice eliminated their infection in 6 to 8 weeks. Male F1 progeny obtained from matings between female CBA/N mice and male BALB/c mice expressed the xid gene and developed prolonged infections. In contrast, all other F1 progeny of CBA/N and BALB/c matings, which did express the xid gene, eliminated G. muris. The link between the xid gene and prolonged infection was confirmed by studies of C57BL/6 mice congenic for the xid gene. When compared with BALB/c or F1 mice, CBA/N mice produced large quantities of immunoglobulin A (IgA) anti-G. muris antibody in serum and gut secretions during prolonged infection. Serum IgG anti-G. muris antibody levels were reduced in CBA/N and F1 male mice that expressed the xid gene. The inability of xid mice to eliminate G. muris is consistent with the importance of antibody in the development of immunity to G. muris. We hypothesize that mice bearing the xid gene fail to produce IgA antibody of appropriate specificity to an antigen or antigens whose recognition by antibody is critical for successful elimination of the parasite.

Published
1988

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