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[Display omitted] • Heterogeneous effects of UGS availability and spatial configuration on LST were explored. • UGS availability is negatively associated with both daytime and nighttime LST. • The Gini coefficient of UGS distribution has a positive effect on solely daytime LST. • The impacts of UGS availability on LST decrease with increased LST quantiles. • The impacts of UGS spatial configuration increase with increased LST quantiles. The impacts of the availability and spatial configuration of urban green spaces (UGS) on their cooling effects can vary with background climate conditions. However, large-scale studies that assess the potential heterogeneous relationships of UGS availability and spatial configuration with urban thermal environment are still lacking. In this study, we investigated the impacts of UGS availability and spatial configuration on urban land surface temperature (LST) taking 306 cities in China as a case study covering a multi-biome-scale. We first calculated the availability of surrounding UGS for urban built-up pixels in each city using a distance-weighted approach, and its spatial configuration was quantified through the Gini coefficient. Then, we employed various regression models to explore how the impacts of UGS availability and the Gini coefficient on LST varies across different LST quantiles and between day- and nighttime. The results revealed that UGS availability was negatively associated with both daytime and nighttime LST, while the Gini coefficient showed a positive impact solely on daytime LST, indicating that an adequate and equally distributed UGS contributes to lower environmental temperatures during the daytime. Furthermore, the impact of UGS availability on LST decreased during both day- and nighttime with increased background LST quantiles. Whereas the impact of the Gini coefficient increased only with daytime LST quantile levels, with its effect remaining almost insignificant during the night. Our findings provide new insights into the impacts of UGS on urban thermal environment, offering significant implications for urban green infrastructure planning aiming at lowering the urban heat island. [ABSTRACT FROM AUTHOR]