1. Increased expression of stress inducible protein 1 in glioma-associated microglia/macrophages.
- Author
-
Carvalho da Fonseca AC, Wang H, Fan H, Chen X, Zhang I, Zhang L, Lima FR, and Badie B
- Subjects
- Animals, Brain Neoplasms metabolism, CX3C Chemokine Receptor 1, Cell Line, Tumor, Disease Progression, Female, Flow Cytometry, Gene Expression immunology, Glioma metabolism, Heat-Shock Proteins genetics, Heat-Shock Proteins metabolism, Lymphocytes immunology, Mice, Mice, Inbred C57BL, Mice, Transgenic, Receptors, Chemokine genetics, Tumor Microenvironment immunology, Brain Neoplasms immunology, Glioma immunology, Heat-Shock Proteins immunology, Macrophages immunology, Microglia immunology
- Abstract
Factors released by glioma-associated microglia/macrophages (GAMs) play an important role in the growth and infiltration of tumors. We have previously demonstrated that the co-chaperone stress-inducible protein 1 (STI1) secreted by microglia promotes proliferation and migration of human glioblastoma (GBM) cell lines in vitro. In the present study, in order to investigate the role of STI1 in a physiological context, we used a glioma model to evaluate STI1 expression in vivo. Here, we demonstrate that STI1 expression in both the tumor and in the infiltrating GAMs and lymphocytes significantly increased with tumor progression. Interestingly, high expression of STI1 was observed in macrophages and lymphocytes that infiltrated brain tumors, whereas STI1 expression in the circulating blood monocytes and lymphocytes remained unchanged. Our results correlate, for the first time, the expression of STI1 and glioma progression, and suggest that STI1 expression in GAMs and infiltrating lymphocytes is modulated by the brain tumor microenvironment., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF