1. Sickle cell disease in a Brazilian population from Sao Paulo: a study of the beta s haplotypes.
- Author
-
Goncalves MS, Nechtman JF, Figueiredo MS, Kerbauy J, Arruda VR, Sonati MF, Saad SO, Costa FF, and Stoming TA
- Subjects
- Base Sequence, Blotting, Southern, Brazil, Gene Frequency, Haplotypes, Humans, Molecular Sequence Data, Polymerase Chain Reaction, Globins genetics, Sickle Cell Trait blood
- Abstract
In this study we have determined the frequency of beta S haplotypes in a Brazilian sickle cell disease population from Sao Paulo, Brazil, by analyzing sequence variations in the immediate 5' flanking and second intervening sequence (IVSII) regions of the gamma globin genes. This association between sequence differences and beta s haplotype backgrounds was determined by screening genomic DNA samples using dot blot analysis of polymerase chain reaction products. We studied 148 beta s chromosomes, and found that haplotype 20 (CAR or Bantu) significantly predominated in this population. This is in agreement with the findings of the historical Portuguese Atlantic slave trade from Africa to South America.
- Published
- 1994
- Full Text
- View/download PDF