1. Effect of glucagon-like peptide 1 (7-36 amide) on insulin-mediated glucose uptake in patients with type 1 diabetes.
- Author
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Meneilly, Graydon S., McIntosh, Christopher H.S., Pederson, Raymond A., Habener, Joel F., Ehlers, Mario R. W., Egan, Josephine M., and Elahi, Dariush
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PEPTIDES ,DIABETES ,DRUG dosage ,BLOOD sugar ,C-peptide ,CLINICAL trials ,COMPARATIVE studies ,GLUCAGON ,HYPERINSULINISM ,INSULIN ,TYPE 1 diabetes ,LIVER ,RESEARCH methodology ,MEDICAL cooperation ,NEUROTRANSMITTERS ,RESEARCH ,GLUCAGON-like peptide 1 ,EVALUATION research ,RANDOMIZED controlled trials ,GLUCAGON-like peptides ,GLUCOSE clamp technique - Abstract
Objective: To examine the insulinomimetic insulin-independent effects of glucagon-like peptide (GLP)-1 on glucose uptake in type 1 diabetic patients.Research Design and Methods: We used the hyperinsulinemic-euglycemic clamp (480 pmol. m(-2) x min(-1)) in paired randomized studies of six women and five men with type 1 diabetes. In the course of one of the paired studies, the subjects also received GLP-1 at a dose of 1.5 pmol. kg(-1) x min(-1). The patients were 41 +/- 3 years old with a BMI of 25 +/- 1 kg/m(2). The mean duration of diabetes was 23 +/- 3 years.Results: Plasma glucose was allowed to fall from a fasting level of approximately 11 mmol/l to 5.3 mmol/l in each study and thereafter was held stable at that level. Plasma insulin levels during both studies were approximately 900 pmol/l. Plasma C-peptide levels did not change during the studies. In the GLP-1 study, plasma total GLP-1 levels were elevated from the fasting level of 31 +/- 3 to 150 +/- 17 pmol/l. Plasma glucagon levels fell from the fasting levels of approximately 14 pmol/l to 9 pmol/l during both paired studies. Hepatic glucose production was suppressed during the glucose clamps in all studies. Glucose uptake was not different between the two studies ( approximately 40 micromol. kg(-1) x min(-1)).Conclusions: GLP-1 does not augment insulin-mediated glucose uptake in lean type 1 diabetic patients. [ABSTRACT FROM AUTHOR]- Published
- 2003
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