1. Glucose oxidase-copper hybrid nanoflowers embedded with magnetic nanoparticles as an effective antibacterial agent.
- Author
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Lee I, Cheon HJ, Adhikari MD, Tran TD, Yeon KM, Kim MI, and Kim J
- Subjects
- Amines chemistry, Biocatalysis, Escherichia coli drug effects, Hydrogen-Ion Concentration, Oxidation-Reduction, Staphylococcus aureus drug effects, Anti-Bacterial Agents chemistry, Anti-Bacterial Agents pharmacology, Copper chemistry, Glucose Oxidase metabolism, Magnetic Iron Oxide Nanoparticles chemistry
- Abstract
Bacterial contamination causes various problems ranging from bacterial infection to biofouling. As an effective and non-toxic agent for bacterial de-contamination, glucose oxidase (GOx)-copper hybrid nanoflowers embedded with amine-functionalized magnetic nanoparticles (NH
2 -MNPs), called 'MNP-GOx NFs', are developed. Positively-charged NH2 -MNPs and negatively-charged GOx molecules are first interacted via electrostatic attraction which can be controlled by changing the buffer pH, and the follow-up addition of copper(II) sulfate leads to blooming of nanoflowers (MNP-GOx NFs) after incubation at room temperature for 3 days. MNP-GOx NFs show effective antibacterial activity by generating H2 O2 from GOx-catalyzed glucose oxidation. For example, 99.9% killings of Staphylococcus aureus and Escherichia coli are achieved after 3 h treatment of 106 /mL cells with 0.2 and 3.0 mg/mL MNP-GOx NFs, respectively, revealing that Gram-positive S. aureus with mono-layer membrane system is more vulnerable to the treatment of MNP-GOx NFs than Gram-negative E. coli with two-layer membrane system. MNP-GOx NFs can maintain 97% of bactericidal activity even after recycled uses by magnetic separation for eight times iterative bacterial killings. Finally, MNP-GOx NFs are employed for the fabrication of antibacterial gauzes. MNP-GOx NFs have also opened up a great potential for their applications in biosensors, biofuel cells and bioconversion as well as bacterial de-contamination., Competing Interests: Declaration of competing interest The authors declare no competing financial interest., (Copyright © 2019 Elsevier B.V. All rights reserved.)- Published
- 2020
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