1. Arabidopsis myrosinases link the glucosinolate-myrosinase system and the cuticle.
- Author
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Ahuja I, de Vos RC, Rohloff J, Stoopen GM, Halle KK, Ahmad SJ, Hoang L, Hall RD, and Bones AM
- Subjects
- Arabidopsis genetics, Arabidopsis ultrastructure, Arabidopsis Proteins genetics, Glucosinolates genetics, Glycoside Hydrolases genetics, Mutation, Plant Stomata genetics, Plant Stomata ultrastructure, Arabidopsis metabolism, Arabidopsis Proteins metabolism, Glucosinolates metabolism, Glycoside Hydrolases metabolism, Plant Stomata metabolism
- Abstract
Both physical barriers and reactive phytochemicals represent two important components of a plant's defence system against environmental stress. However, these two defence systems have generally been studied independently. Here, we have taken an exclusive opportunity to investigate the connection between a chemical-based plant defence system, represented by the glucosinolate-myrosinase system, and a physical barrier, represented by the cuticle, using Arabidopsis myrosinase (thioglucosidase; TGG) mutants. The tgg1, single and tgg1 tgg2 double mutants showed morphological changes compared to wild-type plants visible as changes in pavement cells, stomatal cells and the ultrastructure of the cuticle. Extensive metabolite analyses of leaves from tgg mutants and wild-type Arabidopsis plants showed altered levels of cuticular fatty acids, fatty acid phytyl esters, glucosinolates, and indole compounds in tgg single and double mutants as compared to wild-type plants. These results point to a close and novel association between chemical defence systems and physical defence barriers.
- Published
- 2016
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