1. Menadione metabolism to thiodione in hepatoblastoma by scanning electrochemical microscopy.
- Author
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Mauzeroll J, Bard AJ, Owhadian O, and Monks TJ
- Subjects
- Cell Line, Tumor, Electrochemistry, Glutathione chemistry, Hepatoblastoma pathology, Humans, Inactivation, Metabolic, Liver drug effects, Liver metabolism, Liver pathology, Molecular Structure, Time Factors, Vitamin K 3 chemistry, Vitamin K 3 toxicity, Glutathione analogs & derivatives, Glutathione metabolism, Hepatoblastoma metabolism, Microscopy methods, Vitamin K 3 analogs & derivatives, Vitamin K 3 metabolism
- Abstract
The cytotoxicity of menadione on hepatocytes was studied by using the substrate generation/tip collection mode of scanning electrochemical microscopy by exposing the cells to menadione and detecting the menadione-S-glutathione conjugate (thiodione) that is formed during the cellular detoxication process and is exported from the cell by an ATP-dependent pump. This efflux was electrochemically detected and allowed scanning electrochemical microscopy monitoring and imaging of single cells and groups of highly confluent live cells. Based on a constant flux model, approximately 6 x 10(6) molecules of thiodione per cell per second are exported from monolayer cultures of Hep G2 cells.
- Published
- 2004
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