1. A supramolecular bifunctional artificial enzyme with superoxide dismutase and glutathione peroxidase activities
- Author
-
Yu, Shuangjiang, Huang, Xin, Miao, Lu, Zhu, Junyan, Yin, Yanzhen, Luo, Quan, Xu, Jiayu, Shen, Jiacong, and Liu, Junqiu
- Subjects
- *
SUPEROXIDE dismutase , *GLUTATHIONE , *PEROXIDASE , *SUPRAMOLECULAR chemistry , *CYCLODEXTRINS , *MOLECULAR self-assembly , *PORPHYRINS , *NUCLEAR magnetic resonance spectroscopy - Abstract
Abstract: For constructing a bifunctional antioxidative enzyme with both superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities, a supramolecular artificial enzyme was successfully constructed by the self-assembly of the Mn(III)meso-tetra[1-(1-adamantyl methyl ketone)-4-pyridyl] porphyrin (MnTPyP-M-Ad) and cyclodextrin-based telluronic acid (2-CD-TeO3H) through host–guest interaction in aqueous solution. The self-assembly of the adamantyl moieties of Mn(III) porphyrin and the β-CD cavities of 2-CD-TeO3H was demonstrated by the NMR spectra. In this supramolecular enzyme model, the Mn(III) porphyrin center acted as an efficient active site of SOD and tellurol moiety endowed GPx activity. The SOD-like activity (IC50) of the new catalyst was found to be 0.116μM and equals to 2.56% of the activity of the native SOD. Besides this, supramolecular enzyme model also showed a high GPx activity, and a remarkable rate enhancement of 27-fold compared to the well-known GPx mimic ebselen was observed. More importantly, the supramolecular artificial enzyme showed good thermal stability. [Copyright &y& Elsevier]
- Published
- 2010
- Full Text
- View/download PDF