1. Vatairea guianensis lectin stimulates changes in gene expression and release of TNF-α from rat peritoneal macrophages via glycoconjugate binding.
- Author
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Marques GFO, Pires AF, Osterne VJS, Pinto-Junior VR, Silva IB, Martins MGQ, Oliveira MV, Gomes AM, de Souza LAG, Pavão MSG, Cavada BS, Assreuy AMS, and Nascimento KS
- Subjects
- Animals, Cell Movement drug effects, Cells, Cultured, Disease Models, Animal, Female, Gene Expression Regulation drug effects, Glycoconjugates chemistry, Leukocytes drug effects, Macrophages, Peritoneal metabolism, Peritonitis chemically induced, Peritonitis metabolism, Peritonitis pathology, Plant Lectins chemistry, Plant Lectins metabolism, Rats, Wistar, Receptors, Tumor Necrosis Factor, Type I chemistry, Receptors, Tumor Necrosis Factor, Type I metabolism, Toll-Like Receptor 4 metabolism, Tumor Necrosis Factor-alpha genetics, Tumor Necrosis Factor-alpha metabolism, Rats, Fabaceae chemistry, Glycoconjugates metabolism, Macrophages, Peritoneal drug effects, Plant Lectins pharmacology
- Abstract
Using a rat model of peritonitis, we herein report the inflammatory effect induced by the lectin isolated from Vatairea guianensis (VGL) seeds in the context of interactions between VGL and both toll-like receptor 4 (TLR4) and tumor necrosis factor receptor 1 (TNFR1). Peritoneal macrophages were stimulated with VGL for dose-dependent gene expression and release of TNF-α. In vivo results showed that VGL (1 mg/kg; intraperitoneal) induced peritonitis in female Wistar rats. Leukocyte migration, macrophage activation, and protein leakage were measured 3 and 6 hours after induction. In vitro, peritoneal macrophages were stimulated with VGL for gene expression and TNF-α dosage (mean ± SEM (n = 6), analysis of variance, and Bonferroni's test (P < .05)). In silico, VGL structure was applied in molecular docking with representative glycans. It was found that (a) VGL increases vascular permeability and stimulates leukocyte migration, both rolling and adhesion; (b) lectin-induced neutrophil migration occurs via macrophage stimulation, both in vitro and in vivo; (c) lectin interacts with TLR4 and TNFR1; and (d) stimulates TNF-α gene expression (RT-PCR) and release from peritoneal macrophages. Thus, upon lectin-glycan binding on the cell surface, our results suggest that VGL induces an acute inflammatory response, in turn activating the release of peritoneal macrophages via TNF-α and TLR and/or TNFR receptor pathways., (© 2021 John Wiley & Sons Ltd.)
- Published
- 2021
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