1. Cyto-sensing in electrochemical lab-on-paper cyto-device for in-situ evaluation of multi-glycan expressions on cancer cells
- Author
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Nianqiang Li, Lei Ge, Mei Yan, Shenguang Ge, Xiaoxiao Zheng, Min Su, Jinghua Yu, and Qingkun Kong
- Subjects
Paper ,Working electrode ,Materials science ,Conductometry ,Aptamer ,Biomedical Engineering ,Biophysics ,Nanotechnology ,Biosensing Techniques ,Glycomics ,3D cell culture ,Polysaccharides ,Electrochemistry ,medicine ,Biomarkers, Tumor ,Humans ,Disposable Equipment ,Cancer ,General Medicine ,Equipment Design ,Neoplasms, Experimental ,Microfluidic Analytical Techniques ,medicine.disease ,Equipment Failure Analysis ,Tissue Array Analysis ,Electrode ,Cancer cell ,Differential pulse voltammetry ,Gold ,K562 Cells ,Biotechnology - Abstract
A novel electrochemical lab-on-paper cyto-device (ELPCD) was fabricated to demonstrate sensitive and specific cancer cell detection as well as in-situ monitoring of multi-glycans on living cancer cells. In this ELPCD, aptamers modified three-dimensional macroporous Au-paper electrode (Au-PE) was employed as the working electrode for specific and efficient cancer cell capture. Using a sandwich format, sensitive and reproducible cell detection was achieved in this ELPCD on the basis of the electrochemical signal amplification of the Au-PE and the horseradish peroxidase-lectin electrochemical probe. The ELPCD displayed excellent analytical performance for the detection of four K562 cells with a wide linear calibration range from 550 to 2.0×10(7) cells mL(-1). Then, this ELPCD was successfully applied to determine cell-surface multi-glycans in parallel and in-situ monitor multi-glycans expression on living cells in response to drug treatment through in-electrode 3D cell culture. The proposed method provides promising application in decipherment of the glycomic codes as well as clinical diagnosis and treatment in early process of cancer.
- Published
- 2014