1. Substrate Specificity of NovM: Implications for Novobiocin Biosynthesis and Glycorandomization
- Author
-
John B. Biggins, Heidi R. Vollmer, Jon S. Thorson, Christoph Albermann, William A. Barton, Jacob Lesniak, Aileen Soriano, Dimitar B. Nikolov, and Jiqing Jiang
- Subjects
Natural product ,biology ,Stereochemistry ,Organic Chemistry ,Nucleotide sugar ,biology.organism_classification ,Biochemistry ,Streptomyces ,Sugar nucleotide ,Anti-Bacterial Agents ,Substrate Specificity ,Glycorandomization ,chemistry.chemical_compound ,Biosynthesis ,chemistry ,medicine ,Substrate specificity ,Glycosides ,Physical and Theoretical Chemistry ,Novobiocin ,medicine.drug - Abstract
[reaction: see text] In an effort to expand the scope of natural product in vitro glycorandomization (IVG), the substrate specificity of NovM was investigated. A test of four aglycon analogues and over 40 nucleotide sugars revealed NovM has a surprisingly stringent substrate specificity and provided only three new "unnatural" natural products. On the basis of the determined substrate specificity, an alternative to the sugar nucleotide biosynthetic dogma and a cautionary note for the general applicability of IVG are introduced.
- Published
- 2003