1. Molecular Characterization and Expression Profiles of Sp-uchl3 and Sp-uchl5 during Gonad Development of Scylla paramamosain.
- Author
-
Han K, Dai Y, Zhang Z, Zou Z, and Wang Y
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Brachyura classification, Brachyura growth & development, Cloning, Molecular, Cysteine Endopeptidases chemistry, Gonads growth & development, Models, Molecular, Organ Specificity, Phylogeny, Protein Conformation, Sequence Analysis, DNA, Structure-Activity Relationship, Ubiquitin Thiolesterase chemistry, Brachyura genetics, Cysteine Endopeptidases genetics, Gene Expression Regulation, Developmental, Gonads metabolism, Transcriptome, Ubiquitin Thiolesterase genetics
- Abstract
Ubiquitin C-terminal hydrolases (UCHLs) are a subset of deubiquitinating enzymes, and are involved in numerous physiological processes. However, the role of UCHLs during gonad development has not been studied in crustaceans. In this study, we have first cloned and analyzed expression profiling of Sp-uchl3 and Sp-uchl5 genes from mud crab Scylla paramamosain . The full-length cDNA of Sp-uchl3 is of 1804 bp. Its expression level in the ovary was significantly higher than in other tissues ( p < 0.01), and during gonadal development, its expression in both O1 and O5 stages was significantly higher than in the other three stages of ovaries ( p < 0.05), while in T3 it was higher than in the former two stages of testes ( p < 0.05). Meanwhile, the full-length cDNA of Sp-UCHL5 is 1217 bp. The expression level in the ovary was significantly higher than in other tissues ( p < 0.01). Its expression in ovaries was higher than in testes during gonadal development ( p < 0.05). The expression level in the O5 stage was the highest, followed by the O3 stage in ovarian development, and with no significant difference in the testis development ( p > 0.05). These results provide basic data showing the role of Sp -UCHL3 and Sp -UCHL5 in the gonad development of the crab., Competing Interests: The authors declare no conflicts of interest.
- Published
- 2018
- Full Text
- View/download PDF