Search

Objective To observe the changes in the expression of absent in melanoma 2(AIM2), inflammasome signal pathway-related factors, double-stranded DNA (dsDNA), interleukin (IL)-1β, IL-18 and gasdermin D (GSDMD) in peripheral blood mononuclear cells (PBMCs) of patients with acute gouty arthritis, to analyze the relationship between AIM2, GSDMD in mononuclear cells and the condition of patients with acute gouty arthritis [WBC, PLT, ESR, CRP, IL1β, IL-18, BMI, UA, BUN, serum creatinine (SCr), creatinine clearance rate (CCr), TG, TC], and to explore the role of AIM2 inflammasome signal pathway-mediated pyroptosis in the development and progression of acute gouty arthritis. Methods Thirty patients with acute gouty arthritis (observation group) and 30 healthy controls (control group) were included to compare the levels of AIM2, GSDMD in PBMCs, and IL-1β, IL-18, and dsDNA in serum. Spearman correlation analysis was used to investigate the relationships between AIM2 mRNA, GSDMD mRNA, and the related indicators of patients with acute gouty arthritis (IL-1β, IL-18, WBC, PLT, ESR, CRP, BMI, UA, BUN, SCr, CCr, TG, and TC). Results Compared with the control group, the expression levels of AIM2 and GSDMD mRNA in PBMCs of the observation group were higher (both P<0. 05), and the levels of serum dsDNA, IL-1β, IL-18 were higher (all P<0. 01). AIM2 mRNA in PBMCs was positively correlated with WBC, ESR, CRP, BMI, TC, TG, and SCr (all P<0. 05), and GSDMD mRNA was positively correlated with ESR, IL-1β, and SCr (all P<0. 05). Conclusion The levels of AIM2, GSDMD in PBMCs, and dsDNA, IL-1β, and IL-18 in the serum of patients with acute gouty arthritis increase, indicating that AIM2 inflammasome-mediated pyroptosis may be involved in the development and progression of acute gouty arthritis. [ABSTRACT FROM AUTHOR]