1. Unraveling the nuclear and chloroplast genomes of an agar producing red macroalga, Gracilaria changii (Rhodophyta, Gracilariales).
- Author
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Ho CL, Lee WK, and Lim EL
- Subjects
- Gracilaria classification, Molecular Sequence Annotation, Open Reading Frames, Phylogeny, RNA, Ribosomal genetics, RNA, Transfer genetics, Sequence Homology, Whole Genome Sequencing, Genome, Chloroplast, Gracilaria genetics
- Abstract
Agar and agarose have wide applications in food and pharmaceutical industries. Knowledge on the genome of red seaweeds that produce them is still lacking. To fill the gap in genome analyses of these red algae, we have sequenced the nuclear and organellar genomes of an agarophyte, Gracilaria changii. The partial nuclear genome sequence of G. changii has a total length of 35.8Mb with 10,912 predicted protein coding sequences. Only 39.4% predicted proteins were found to have significant matches to protein sequences in SwissProt. The chloroplast genome of G. changii is 183,855bp with a total of 201 open reading frames (ORFs), 29 tRNAs and 3 rRNAs predicted. Five genes: ssrA, leuC and leuD CP76_p173 (orf139) and pbsA were absent in the chloroplast genome of G. changii. The genome information is valuable in accelerating functional studies of individual genes and resolving evolutionary relationship of red seaweeds., (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Published
- 2018
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