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The information regarding relationship between nematode dosage and infective juveniles (IJ) production are available for Steinernema affine. Therefore, in the present study the effect of inoculation doses of S. affine and extraction methods was investigated on the production of IJ in Galleria mellonella. There was significantly greater emergence of IJ of S. affine from the Galleria mellonella using White traps than with modified Baermann extraction trays. In Baermann extraction trays, the maximum emergence of IJ of S. affine was observed at an inoculation dose of 400 IJ followed by the dose of 50 IJ. The minimum emergence was found with an inoculation dose of 100 IJ. On the other hand, in case of White traps, the maximum emergence of IJ was recorded with doses of 100 and 50 IJ. The minimum emergence of IJ was observed with a dose of 200 IJ. The relationships between inoculation doses and emergence of IJ from the cadavers for both the methods were found to be non-significant. [ABSTRACT FROM AUTHOR]

The management of insect pests is mainly relied on synthetic insecticides which are inimical to humans, livestock and environment. To dispense with the pernicious consequences of chemicals, use of entomopathogenic nematodes (EPNs) is among the viable alternatives. The successful management by EPNs is dependent on their establishment in soils, infectivity, persistence and pathogenicity for longer periods. In the present study, emergence of Steinernema feltiae infective juveniles (IJs) from infected Galleria mellonella cadavers was monitored under moist and dry conditions at 5 and 10°C. Greater numbers of IJs of S. feltiae recovered from G. mellonella cadavers kept at 10°C than from those kept at 5°C. Likewise, significantly greater number of infective juveniles emerged in moist conditions as compared to dry. The relationship between both the temperatures and wet and dry conditions was highly significant (P<0.001). [ABSTRACT FROM AUTHOR]

Entomopathogenic nematodes (EPN) are one of the promising alternatives to synthetic insecticides for the control of insect pests. For successful insect management, EPNs must be established in the soil and remain infective, persistent and pathogenic for long periods. The present study investigated on the emergence of Steinernema feltiae infective juveniles (IJ) from infected Galleria mellonella cadavers in moist and dry conditions. A significantly high number of infective juveniles of S. feltiae emerged in moist conditions as compared to dry. A positive correlation was found between the weight of the Galleria larvae at infection and the numbers of IJ recovered in the moist as compared with dry conditions. [ABSTRACT FROM AUTHOR]

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