1. Apelin (65-77) activates extracellular signal-regulated kinases via a PTX-sensitive G protein.
- Author
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Masri B, Lahlou H, Mazarguil H, Knibiehler B, and Audigier Y
- Subjects
- Adipokines, Animals, Apelin, Apelin Receptors, CHO Cells, Cricetinae, Cyclic AMP-Dependent Protein Kinases chemistry, Dose-Response Relationship, Drug, Endothelium metabolism, Enzyme Activation, Enzyme Inhibitors pharmacology, Flavonoids pharmacology, Genes, Dominant, Immunoblotting, Indoles pharmacology, Intercellular Signaling Peptides and Proteins, Maleimides pharmacology, Mice, Mitogen-Activated Protein Kinase 1 metabolism, Mitogen-Activated Protein Kinase 3, Monomeric GTP-Binding Proteins metabolism, Mutation, Peptide Fragments chemistry, Phosphorylation, Plasmids metabolism, Protein Binding, Protein Isoforms, Protein Structure, Tertiary, Receptors, Cell Surface biosynthesis, Receptors, Dopamine D2 biosynthesis, Signal Transduction, Time Factors, Transfection, Type C Phospholipases metabolism, Carrier Proteins chemistry, Carrier Proteins metabolism, GTP-Binding Proteins metabolism, Mitogen-Activated Protein Kinases metabolism, Pertussis Toxin, Receptors, G-Protein-Coupled, Recombinant Proteins, Virulence Factors, Bordetella metabolism
- Abstract
We report here that apelin (65-77) induces activation of extracellular-regulated kinases (ERKs) in Chinese hamster ovary (CHO) cells expressing the msr/apj receptor. This concentration-dependent activation was transient, peaking at 5 min. Pretreatment of CHO cells with pertussis toxin fully abrogated ERK phosphorylation, whereas overexpression of the beta-adrenergic receptor kinase-1 C-terminal fragment did not alter ERK activation. Transfection with a dominant-negative mutant of Ras was without effect on ERK activation, whereas an inhibitor of many protein kinase C isoforms, GF109203X, strongly decreased it. These results demonstrate that stimulation of the murine msr/apj receptor promotes ERK activation via the alpha subunit of a pertussis toxin-sensitive protein in a Ras-independent pathway., ((c)2002 Elsevier Science.)
- Published
- 2002
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