11 results on '"Li, Shengjie"'
Search Results
2. Akkermansia muciniphila alleviates high‐fat‐diet‐related metabolic‐associated fatty liver disease by modulating gut microbiota and bile acids.
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Wu, Wenrui, Kaicen, Wang, Bian, Xiaoyuan, Yang, Liya, Ding, Shi, Li, Yating, Li, Shengjie, Zhuge, Aoxiang, and Li, Lanjuan
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FATTY liver ,BILE acids ,GUT microbiome ,HIGH-fat diet ,LOW-fat diet ,WEIGHT gain ,DIETARY supplements - Abstract
It has been reported that Akkermansia muciniphila improves host metabolism and reduces inflammation; however, its potential effects on bile acid metabolism and metabolic patterns in metabolic‐associated fatty liver disease (MAFLD) are unknown. In this study, we have analysed C57BL/6 mice under three feeding conditions: (i) a low‐fat diet group (LP), (ii) a high‐fat diet group (HP) and (iii) a high‐fat diet group supplemented with A. muciniphila (HA). The results found that A. muciniphila administration relieved weight gain, hepatic steatosis and liver injury induced by the high‐fat diet. A. muciniphila altered the gut microbiota with a decrease in Alistipes, Lactobacilli, Tyzzerella, Butyricimonas and Blautia, and an enrichment of Ruminiclostridium, Osclibacter, Allobaculum, Anaeroplasma and Rikenella. The gut microbiota changes correlated significantly with bile acids. Meanwhile, A. muciniphila also improved glucose tolerance, gut barriers and adipokines dysbiosis. Akkermansia muciniphila regulated the intestinal FXR‐FGF15 axis and reshaped the construction of bile acids, with reduced secondary bile acids in the caecum and liver, including DCA and LCA. These findings provide new insights into the relationships between probiotics, microflora and metabolic disorders, highlighting the potential role of A. muciniphila in the management of MAFLD. [ABSTRACT FROM AUTHOR]
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- 2023
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3. Neuroprotective effects of an engineered Escherichia coli Nissle 1917 on Parkinson's disease in mice by delivering GLP‐1 and modulating gut microbiota.
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Wu, Heng, Wei, Jing, Zhao, Xiumiao, Liu, Ying, Chen, Zhihang, Wei, Kehong, Lu, Jiachen, Chen, Wenjie, Jiang, Meixiu, Li, Shengjie, and Chen, Tingtao
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PARKINSON'S disease ,GUT microbiome ,ESCHERICHIA coli ,PROTEOLYSIS ,ORAL drug administration ,INSULIN - Abstract
Considerable evidence suggests that insulin resistance is closely linked to Parkinson's disease (PD), leading to agents aiming at treating diabetes can be regarded as new neuroprotective strategies in PD, notably glucagon‐like peptide‐1 (GLP‐1). However, the extremely short half‐life of GLP‐1 due to degradation by the ubiquitous proteolytic enzyme limits its clinical application. In this study, we engineered the recombinant integrant probiotic strain Escherichia coli Nissle 1917 (EcN) to create a strain EcN‐GLP‐1 that effectively delivers the heterologous GLP‐1 molecule. Subsequently, we assessed its neuroprotective effects on 1‐methyl‐4‐phenyl‐1, 2, 3, 6‐tetrahydropyridine (MPTP)‐induced PD mice. We demonstrated that EcN‐GLP‐1 treatment could improve motor deficits, increase tyrosine hydroxylase‐positive neurons, suppress microglia and astrocyte activation, reduce brain and colon inflammation, and ameliorate colonic barrier function damaged by MPTP induction. Meanwhile, we confirmed that the oral administration of EcN‐GLP‐1 could restore the disturbance of gut microbiota in the MPTP‐induced PD mice, by reducing the relative abundances of Akkermansia and Oscillospira, and increasing the level of Prevotella in the gut. These results support further development of an engineered probiotic platform in which production of GLP‐1 for gut‐brain disorders, such as PD. [ABSTRACT FROM AUTHOR]
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- 2023
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4. Bifidobacterium longum R0175 protects mice against APAP-induced liver injury by modulating the Nrf2 pathway.
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Li, Shengjie, Zhuge, Aoxiang, Xia, Jiafeng, Wang, Shuting, Lv, Longxian, Wang, Kaicen, Jiang, Huiyong, Yan, Ren, Yang, Liya, Bian, Xiaoyuan, Jiang, Xianwan, Wang, Qiangqiang, Han, Shengyi, and Li, Lanjuan
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BIFIDOBACTERIUM longum , *LIVER injuries , *NUCLEAR factor E2 related factor , *GUT microbiome , *OXIDATIVE stress , *BIFIDOBACTERIUM , *LIVER cells - Abstract
Acetaminophen (APAP) overdose is the most common driver of drug-induced liver injury (DILI) worldwide, and the gut microbiome plays a crucial role in this process. In this study, we estimated the effect of Bifidobacterium longum R0175 on APAP-induced liver injury in mice and discovered that B. longum R0175 alleviated liver injury by diminishing inflammation, reducing oxidative stress levels, inhibiting hepatocyte death and improving APAP-induced microbiome dysbiosis. Further studies revealed that the antioxidative effects of B. longum R0175 were primarily due to activation of the Nrf2 pathway, which was supported by the Nrf2 pathway inhibitor ML385 counteracting these ameliorative effects. B. longum R0175 modified intestinal metabolites, especially the key metabolite sedanolide, which could activate the Nrf2 pathway and contribute to the protective effects against APAP-induced liver injury. Moreover, we found that sedanolide exhibited close interrelationships with specific microbial taxa, indicating that this factor may be derived from gut microbes. In conclusion, our work demonstrated that B. longum R0175 could reduce oxidative damage, inflammation and hepatocyte death by activating the Nrf2 pathway. Importantly, we identified the microbiota-derived metabolite sedanolide, which was first discovered in the mouse intestine, as a key agonist of the Nrf2 pathway and primary effector of B. longum R0175 in APAP challenge. These findings provide new perspectives for APAP overdose therapy and demonstrate the enormous potential of B. longum R0175 in alleviating acute liver injury. [Display omitted] • B. longum R0175 alleviated APAP-induced liver injury by diminishing inflammation, oxidative stress levels and hepatocyte death. • B. longum R0175 modified key metabolite sedanolide which could activate the Nrf2 pathway and exert protective effects. • Sedanolide was identified as a microbiota-derived metabolite for its correlation with specific microbes. [ABSTRACT FROM AUTHOR]
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- 2023
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5. Characteristics of the intestinal bacterial microbiota profiles in Bifidobacterium pseudocatenulatum LI09 pre-treated rats with D-galactosamine-induced liver injury.
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Zha, Hua, Xia, Jiafeng, Si, Guinian, Tang, Ruiqi, Li, Shengjie, Li, Qian, Lou, Yiqing, Wo, Wanlong, Chang, Kevin, and Li, Lanjuan
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GUT microbiome ,LIVER injuries ,BIFIDOBACTERIUM ,RATS ,CLUSTER analysis (Statistics) ,DYSBIOSIS - Abstract
Bifidobacterium pseudocatenulatum LI09 could prevent D-galactosamine-induced liver injury. Our previous study has preliminarily determined that different intestinal microbiota profiles existed in the LI09-treated rats. Due to the sample size limitation, some subsequent analyses could not be achieved. In the current study, we conducted different experiments and bioinformatic analyses to characterise the distinct intestinal bacterial microbiota profiles in the LI09-treated rats with liver injury (i.e., LI09 group). Partition around medoids clustering analysis determined two intestinal microbiota profiles (i.e., Cluster_1_LI09 and Cluster_2_LI09) in LI09 group. Compared with Cluster_2_LI09, Cluster_1_LI09 group was determined at less dysbiotic microbial status and with lower level of liver injury. The two microbiota profiles were determined with distinct representative amplicon sequence variants (ASVs), among which, ASV1_Akkermansia and ASV3_Bacteroides were most associated with Cluster_1_LI09 and Cluster_2_LI09, respectively. Multiple representative phylotypes in Cluster_1_LI09 negatively correlating with liver function variables were assigned to Parabacteroides, suggesting Parabacteroides could benefit LI09 on modulating the liver function. In addition, ASV310_Lachnospiraceae, ASV501_Muribaculaceae and ASV484_Lachnospiraceae were determined as network gatekeepers in Cluster_1_LI09 network. The relevant results suggest that some intestinal bacteria could assist LI09 in lowering the intestinal microbial dysbiosis in the rats with liver injury, and their clinical application deserves further investigation. [ABSTRACT FROM AUTHOR]
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- 2023
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6. Identification of a protective Bacteroides strain of alcoholic liver disease and its synergistic effect with pectin.
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Wang, Qiangqiang, Li, Yating, Lv, Longxian, Jiang, Huiyong, Yan, Ren, Wang, Shuting, Lu, Yanmeng, Wu, Zhengjie, Shen, Jian, Jiang, Shiman, Lv, Jiawen, Li, Shengjie, Zhuge, Aoxiang, and Li, Lanjuan
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PECTINS ,BACTEROIDES ,BACTEROIDES fragilis ,GUT microbiome ,FATTY liver ,SYNBIOTICS - Abstract
The depletion of Bacteroides in the gut is closely correlated with the progression of alcoholic liver disease (ALD). This study aimed to identify Bacteroides strains with protective effects against ALD and evaluate the synergistic effects of Bacteroides and pectin in this disease. Mice were fed Lieber-DeCarli alcohol diet to establish an experimental ALD model and pre-treated with 4 Bacteroides strains. The severity of the liver injury, hepatic steatosis, and inflammation was evaluated through histological and biochemical assays. We found that Bacteroides fragilis ATCC25285 had the best protective effects against ALD strains by alleviating both ethanol-induced liver injury and steatosis. B. fragilis ATCC25285 could counteract inflammatory reactions in ALD by producing short-chain fat acids (SCFAs) and enhancing the intestinal barrier. In the subsequent experiment, the synbiotic combination of B. fragilis ATCC25285 and pectin was evaluated and the underlying mechanisms were investigated by metabolomic and microbiome analyses. The combination elicited superior anti-ALD effects than the individual agents used alone. The synergistic effects of B. fragilis ATCC25285 and pectin were driven by modulating gut microbiota, improving tryptophan metabolism, and regulating intestinal immune function. Based on our findings, the combination of B. fragilis ATCC25285 and pectin can be considered a potential treatment for ALD. Key points: • B. fragilis ATCC25285 was identified as a protective Bacteroides strain against ALD. • The synbiotic combination of B. fragilis and pectin has better anti-ALD effects. • The synbiotic combination modulates gut microbiota and tryptophan metabolism. [ABSTRACT FROM AUTHOR]
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- 2022
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7. Editorial: The Role of Dietary Interventions in The Regulation of Host-Microbe Interactions.
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Li, Shengjie, Zhao, Xiumiao, and Chen, Tingtao
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PROBIOTICS ,HIGH-fiber diet ,GUT microbiome ,MILKFAT - Published
- 2022
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8. Probiotic Supplement Preparation Relieves Test Anxiety by Regulating Intestinal Microbiota in College Students.
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Qin, Qi, Liu, Hui, Yang, Yulu, Wang, Yifei, Xia, Chaofei, Tian, Puyuan, Wei, Jing, Li, Shengjie, and Chen, Tingtao
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TEST anxiety ,PROBIOTICS ,GUT microbiome ,HAMILTON Depression Inventory ,COLLEGE students ,CHINESE students ,CLOSTRIDIUM perfringens ,CLOSTRIDIUM acetobutylicum - Abstract
Test anxiety creates barriers to learning and performance, which further affects students' social, behavioural, and emotional development. Currently, the medication to treat test anxiety has not been reported yet. Here, we enrolled 120 students to evaluate the effect of probiotic supplement preparation (PSP) on test anxiety from the aspect of the intestinal microbiota. We found that the intake of PSP alleviated the symptoms of depression and anxiety in students with test anxiety by evaluating their mental state using the Hamilton Depression Rating Scale and Hamilton Anxiety Scale. High-throughput sequencing results indicated that the consumption of PSP increased the abundance of Streptococcus and Akkermansia that was lowered by the anxiety state in the intestinal microbiota of students. Meanwhile, taking PSP reduced the level of intestinal pathogens of Fusobacterium and Clostridium as well. In conclusion, our work shows that PSP can reduce test anxiety and restore the disturbed microbiota to the standard level in Chinese college students, rendering the use of PSP a promising strategy for test anxiety. [ABSTRACT FROM AUTHOR]
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- 2021
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9. Editorial: Gut Microbiota in the Occurrence, Development and Treatment of Gut-Brain Disorders.
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Li, Shengjie, Wei, Jing, and Chen, Tingtao
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GUT microbiome ,CHILDREN with autism spectrum disorders ,PHYSIOLOGY ,PROBIOTICS ,MEDICAL research - Abstract
Keywords: gut microbiota; psychiatric disorders; gut-brain axis; stroke; fecal microbiota transplantation; psychobiotics; prebiotics EN gut microbiota psychiatric disorders gut-brain axis stroke fecal microbiota transplantation psychobiotics prebiotics 1 3 3 12/17/21 20211215 NES 211215 Increasingly, it is recognized that symbiotic microorganisms, specifically the microbiota resident in the gut, may influence neurodevelopment and programming of social behaviors among animal species and humanity ([3]). Although several communication pathways contribute to the gut microbiota on brain physiology and behavior, the deep mechanisms by which enteric bacteria communicate with the brain and how the gut microbiota influences the brain causally are still needed to be illustrated. Gut microbiota, psychiatric disorders, gut-brain axis, stroke, fecal microbiota transplantation, prebiotics, psychobiotics. [Extracted from the article]
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- 2021
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10. Effects of partial reduction of polystyrene micro-nanoplastics on the immunity, gut microbiota and metabolome of mice.
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Zha, Hua, Tang, Ruiqi, Li, Shengjie, Zhuge, Aoxiang, Xia, Jiafeng, Lv, Jiawen, Wang, Shuting, Wang, Kaiceng, Zhang, Hua, and Li, Lanjuan
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GUT microbiome , *POLYSTYRENE , *BACTERIAL diversity , *MICE , *IMMUNITY - Abstract
Microplastic (MP) and nanoplastic (NP) could cause gut microbiota alterations. Although micro/nanoplastic (MNP) degradation is attracting increasing scientific interest, the evaluation of MNP reduction in gut needs to be further investigated. This study aimed to determine whether partial reduction of polystyrene MNP in gut could affect the immunity, gut microbiota and metabolome of mice. Serum eotaxin/CCL11 was at a lower level in the mice exposed to 200 μg and 500 μg NP (i.e., 2NP and 5NP groups, respectively) compared to those exposed to 500 μg MP (i.e., 5 MP group), while serum IL-2 and IL-4 were both greater in the 5NP group compared to the 5 MP group. The gut bacterial alpha diversity, fungal diversity and evenness were all similar among the MNP and control groups. However, the gut fungal richness was greater in both the 5NP and 5 MP groups compared to the control group. The gut bacterial and fungal compositions were both different between the MNP and control groups. Multiple gut bacteria and fungi showed different levels between the 2NP and 5NP groups, as well as between the 2NP and 5 MP groups. Increased Staphylococcus and decreased Glomus were determined in the 2NP group compared to both the 5NP and 5 MP groups. A Lactobacillus phylotype was found as the sole gatekeeper in the bacterial network of the 2NP group, while a Bifidobacterium phylotype contributed most to the stability of the bacterial networks of both the 5NP and 5 MP groups. Multiple differential gut metabolic pathways were found between the 2NP and 5NP/5 MP groups, and mTOR signaling pathway was largely upregulated in the 2NP group compared to both the 5NP and 5 MP groups. The relevant results could help with the evaluation of partial reduction of MNP in gut. [Display omitted] • MNP partial reduction could affect mouse immunity. • MNP partial reduction could induce gut bacterial and fungal dysbiosis. • MNP partial reduction could induce gut metabolic disruption in mice. [ABSTRACT FROM AUTHOR]
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- 2024
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11. Alterations of gut and oral microbiota in the individuals consuming take-away food in disposable plastic containers.
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Zha, Hua, Lv, Jiawen, Lou, Yiqing, Wo, Wanlong, Xia, Jiafeng, Li, Shengjie, Zhuge, Aoxiang, Tang, Ruiqi, Si, Nian, Hu, Zhihao, Lu, Haifeng, Chang, Kevin, Wang, Chenyu, Si, Guinian, and Li, Lanjuan
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GUT microbiome , *PLASTIC containers , *MICE , *DYSBIOSIS , *THIOBACILLUS , *CONSUMERS - Abstract
Microplastics (MP) and nanoplastics (NP) exist in the disposable plastic take-away containers. This study aims to determine the gut and oral microbiota alterations in the individuals frequently and occasionally consuming take-away food in disposable plastic containers (TFDPC), and explore the effect of micro/nanoplastics (MNP) reduction on gut microbiota in mice. TFDPC consumption are associated with greater presences of gastrointestinal dysfunction and cough. Both occasional and frequent consumers have altered gut and oral microbiota, and their gut diversity and evenness are greater than those of non-TFDPC consuming cohort. Multiple gut and oral bacteria are associated with TFDPC consumers, among which intestinal Collinsella and oral Thiobacillus are most associated with the frequent consumers, while intestinal Faecalibacterium is most associated with the occasional consumers. Although some gut bacteria associated with the mice treated with 500 µg NP and 500 µg MP are decreased in the mice treated with 200 µg NP, the gut microbiota of the three MNP groups are all different from the control group. This study demonstrates that TFDPC induces gut and oral microbiota alterations in the consumers, and partial reduction of the size and amount of MNP cannot rectify the MNP-induced gut microbial dysbiosis. [Display omitted] • Take-away food in disposable plastic containers (TFDPC) could induce gut and oral microbial alterations in humans. • Multiple bacteria were associated with gut and oral microbial alterations induced by TFDPC. • Partial reduction of the size and amount of MNP cannot rectify the MNP-induced gut microbial dysbiosis. [ABSTRACT FROM AUTHOR]
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- 2023
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