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Development of systematic methods for conservation planning has improved effectiveness and efficiency of implementing such plans. The lesser prairie-chicken (Tympanuchus pallidicinctus) is a grouse species of conservation concern native to the southwestern Great Plains of the United States. Recent lesser prairie-chicken conservation planning has involved identifying ecologically important areas but has not incorporated economic data into prioritization of areas to target for conservation management. We used the program Marxan to develop a decision-support tool for managers in Kansas to prioritize tracts for improving lesser prairie-chicken habitat quality and increasing habitat availability. We developed three different conservation scenarios and evaluated the tradeoffs among multiple planning objectives in these scenarios. We incorporated population targets from an existing conservation plan and agricultural economic data to help select land with maximum ecological value and minimum economic productivity to prioritize for lesser prairie-chicken conservation. We compared potential conservation plans and incorporated a post hoc connectivity model to test potential for individuals to travel among habitat patches in these plans during dispersal events. We found that different conservation scenarios led to different solutions, though differences varied by ecoregion. Potential solutions for all scenarios contained habitat patches not currently included in existing conservation plans and had high connectivity potential. These results provide context for spatial prioritization of lesser prairie-chicken habitat management in Kansas. Application of this approach to species of conservation interest could help managers incorporate socioeconomic factors into planning methods and identify important tracts for conservation currently overlooked by existing planning methods.

The Rainwater Basin in south-central Nebraska once supported a complex network of ~ 12,000 spatially-isolated playa wetlands, but ~ 90% have been lost since European settlement. Future losses are likely and expected reductions in connectivity could further isolate populations, increasing local extinction rates of many wetland species. However, to what extent future losses will affect wildlife likely depends on the role of lost wetlands in maintaining connectivity. We compared the current Rainwater Basin network to future wetland loss scenarios to assess minimum, mean, and maximum effects of losses on network connectivity for a range of wildlife taxa. We used network models to rank wetlands by their functionality and relative importance in maintaining connectivity. We then removed 10–50% of high-ranked, low-ranked, or random subsets of wetlands and assessed connectivity of the remaining network. A 10% loss of highly-ranked wetlands substantially decreased connectivity for species with dispersal capabilities

Large-scale declines of grassland ecosystems in the conterminous United States since European settlement have led to substantial loss and fragmentation of lesser prairie-chicken (Tympanuchus pallidicinctus) habitat and decreased their occupied range and population numbers by ∼85%. Breeding season space use is an important component of lesser prairie-chicken conservation, because it could affect both local carrying capacity and population dynamics. Previous estimates of breeding season space use are largely limited to one of the four currently occupied ecoregions, but potential extrinsic drivers of breeding space use, such as landscape fragmentation, vegetation structure and composition, and density of anthropogenic structures, can show large spatial variation. Moreover, habitat needs vary greatly among the lekking/prelaying, nesting, brood-rearing, and postbreeding stages of the breeding season, but space use by female lesser prairie-chickens during these stages remain relatively unclear. We tested whether home range area and daily displacement (the net distance between the first and last location of each day) of female lesser prairie-chickens varied among ecoregions and breeding stages at four study sites in Kansas and Colorado, U.S.A., representing three of the four currently occupied ecoregions. We equipped females with very-high-frequency (VHF) or Global Positioning System (GPS) transmitters, and estimated home range area with kernel density estimators or biased random bridge models, respectively. Across all ecoregions, breeding season home range area averaged 190.4 ha (±19.1 ha se) for birds with VHF and 283.6 ha (±23.1 ha) for birds with GPS transmitters, whereas daily displacement averaged 374.8 m (±14.3 m). Average home range area and daily displacement of bird with GPS transmitters were greater in the Short-Grass Prairie/ Conservation Reserve Program Mosaic and Sand Sagebrush Prairie Ecoregions compared to sites in the Mixed-Grass Prairie Ecoregion. Home range area and daily displacement were greatest during lekking/prelaying and smallest during the brood-rearing stage, when female movements were restricted by mobility of chicks. Ecoregion- and breeding stage-specific estimates of space use by lesser prairie-chickens will help managers determine the spatial configuration of breeding stage-specific habitat on the landscape. Furthermore, ecoregion- and breeding stage-specific estimates are crucial when estimating the amount of breeding habitat needed for lesser prairie-chicken populations to persist.

Loss and degradation of grasslands in the Great Plains region have resulted in major declines in abundance of grassland bird species. To ensure future viability of grassland bird populations, it is crucial to evaluate specific effects of environmental factors among species to determine drivers of population decline and develop effective conservation strategies. We used threshold models to quantify the effects of land cover and weather changes in "lesser prairie‐chicken" and "greater prairie‐chicken" (Tympanuchus pallidicinctus and T. cupido, respectively), northern bobwhites (Colinus virginianus), and ring‐necked pheasants (Phasianus colchicus). We demonstrated a novel approach for estimating landscape conditions needed to optimize abundance across multiple species at a variety of spatial scales. Abundance of all four species was highest following wet summers and dry winters. Prairie chicken and ring‐necked pheasant abundance was highest following cool winters, while northern bobwhite abundance was highest following warm winters. Greater prairie chicken and northern bobwhite abundance was also highest following cooler summers. Optimal abundance of each species occurred in landscapes that represented a grassland and cropland mosaic, though prairie chicken abundance was optimized in landscapes with more grassland and less edge habitat than northern bobwhites and ring‐necked pheasants. Because these effects differed among species, managing for an optimal landscape for multiple species may not be the optimal scenario for any one species., We quantified the effects of land cover and weather changes in Galliformes in Kansas. We found evidence of nonlinear, threshold effects of land cover change in abundance of all four focal species, suggesting that the presence of both grassland and cropland is important in the landscape to simultaneously maintain these populations.

The Rainwater Basin region in south-central Nebraska supports a complex network of spatially-isolated wetlands that harbor diverse floral and faunal communities. Since European settlement, many wetlands have been lost from the network, which has increased distances among remaining wetlands. As a result, populations of wildlife species with limited dispersal capabilities may have become isolated and face greater local extinction risks. We compared the pre-European settlement and current extent of the Rainwater Basin network to assess the effects of wetland losses on network connectivity for a range of maximum dispersal distances. We constructed network models for a range of maximum dispersal distances and calculated network metrics to assess changes in network connectivity and the relative importance of individual wetlands in regulating flow. Since European settlement, the number of wetlands in the Rainwater Basin has decreased by > 90%. The average distance to the nearest neighboring wetland has increased by 150% to ~ 1.2 km, and the dispersal distance necessary to travel throughout the whole network has increased from 3.5 to 10.0 km. Last, relative importance of individual wetlands depended on the maximum dispersal distance. Which wetlands to preserve to maintain connectivity might therefore depend on the dispersal capabilities of the species or taxa of interest. To preserve a broad range of biodiversity, conservation efforts should focus on preserving dense clusters of wetlands at fine spatial scales to maintain current levels of network connectivity, and restoring connections between clusters to facilitate long-range dispersal of species with limited dispersal capabilities.

Coastal wetlands along the Gulf of Mexico support a wide diversity of wildlife, are important nurseries for sport and commercial fisheries, provide erosion and flood control, and serve many other ecological functions and services. These marshes have been declining in area and degrading at alarming rates since the 1930s. Effective conservation planning is vital to protect these ecosystems, but decision makers often lack knowledge of expected future conditions to strategically target conservation actions. To address this issue, we focus on a species of conservation concern, the mottled duck (Anas fulvigula), that resides year-round in the coastal marshes of the Gulf of Mexico. We used location data collected from radiomarked hen mottled ducks from 2006 to 2011 to create an ensemble model of habitat selection for 2010. We then projected future habitat states using models of sea-level rise and human development. By combining future predictions with our ensemble model, we predict future habitat for mottled ducks through 2100, in 20-year time steps beginning with 2020. Sea-level rise models predicted reductions in coastal marsh habitats and our ensemble model predict corresponding declines in overall habitat quantity and quality for mottled ducks, with the largest rate of habitat loss predicted within the Chenier Plain of Louisiana, USA at 71%. In some areas, particularly the Texas Mid-Coast, USA, future urbanization and human development is expected to reduce the ability of wetland habitat to migrate inland with rising sea-levels. Our results also highlight areas of coastal marsh particularly vulnerable to sea-level rise; and conversely, identify areas most likely to persist into the future that could be targeted for habitat conservation to help mottled ducks persist on the landscape.

Understanding the relative influence of environmental and intrinsic effects on populations is important for managing and conserving harvested species, especially those species inhabiting changing environments. Additionally, climate change can increase the uncertainty associated with management of species in these changing environments, making understanding factors affecting their populations even more important. Coastal ecosystems are particularly threatened by climate change; the combined effects of increasing severe weather events, sea level rise, and drought will likely have non-linear effects on coastal marsh wildlife species and their associated habitats. A species of conservation concern that persists in these coastal areas is the mottled duck (Anas fulvigula). Mottled ducks in the western Gulf Coast are approximately 50% below target abundance numbers established by the Gulf Coast Joint Venture for Texas and Louisiana, USA. Although evidence for declines in mottled duck abundance is apparent, specific causes of the decrease remain unknown. Our goals were to determine where the largest declines in mottled duck population were occurring along the system of Texas Gulf Coast National Wildlife Refuges and quantify the relative contribution of environmental and intrinsic effects on changes to relative population density. We modeled aerial survey data of mottled duck density along the Texas Gulf Coast from 1986–2015 to quantify effects of extreme weather events on an index to mottled duck density using the United States Climate Extremes Index and Palmer Drought Severity Index. Our results indicate that decreases in abundance are best described by an increase in days with extreme 1-day precipitation from June to November (hurricane season) and an increase in drought severity. Better understanding those portions of the life cycle affected by environmental conditions, and how to manage mottled duck habitat in conjunction with these events will likely be key to persistence of the species under future environmental conditions. © 2017 The Wildlife Society.

The mottled duck (Anas fulgivula) is a non-migratory duck dependent on coastal habitats to meet all of its life cycle requirements in the Western Gulf Coast (WGC) of Texas and Louisiana, USA. This population of mottled ducks has experienced a moderate decline during the past 2 decades. Adult survival has been identified as an important factor influencing population demography. Previous work based on band-recovery data has provided only annual estimates of survival. We assessed seasonal patterns of female mottled duck survival from 2009 to 2012 using individuals marked with satellite platform transmitter terminals (PTTs). We used temperature and movement sensors within each PTT to indicate potential mortality events. We estimated cumulative weekly survival and ranked factors influential in patterns of mortality using known-fate modeling in Program MARK. Models included 4 predictors: week; hunting and non-hunting periods; biological periods defined as breeding, brooding, molt, and pairing; and mass at time of capture. Models containing hunt periods, during and outside the mottled duck season, comprised essentially 100% of model weights where both legal and illegal harvest had a negative influence on mottled duck survival. Survival rates were low during 2009–2011 (12–38% annual rate of survival), when compared with the long-term banding average of 53% annual survival. During 2011, survival of female mottled ducks was the lowest annual rate (12%) ever documented and coincided with extreme drought. Management actions maximizing the availability of wetlands and associated upland habitats during hunting seasons and drought conditions may increase adult female mottled duck survival. © 2017 The Authors. Journal of Wildlife Management Published by Wiley Periodicals, Inc. on behalf of The Wildlife Society.

Since the beginning of the 20th century, the overall occupied range of the lesser prairie-chicken (Tympanuchus pallidicinctus) has declined by 84% commensurate with population trends. Much of this decline has been attributed to the loss and fragmentation of native grasslands throughout the lesser prairie-chicken range. However, quantification of changes in land cover in the distribution of the lesser prairie-chicken is lacking. Our objectives were to (1) document changes in the areal extent and connectivity of grasslands in the identified lesser prairie-chicken range in Kansas, USA, (>60% of extant lesser prairie-chicken population) from the 1950s to 2013 using remotely sensed data and (2) assess the potential of the Conservation Reserve Program (U.S. Department of Agriculture Program converting cropland to permanent cover; CRP) to mitigate grassland loss. Digital land cover maps were generated on a decadal time step through spectral classification of LANDSAT images and visual analysis of aerial photographs (1950s and 1960s). Landscape composition and configuration were assessed using FRAGSTATS to compute a variety of landscape metrics measuring changes in the amount of grassland present as well as changes in the size and configuration of grassland patches. With the exception of a single regional portion of the range, nearly all of the grassland converted to cropland in the lesser prairie-chicken range of Kansas occurred prior to the 1950s. Prior to the implementation of CRP, the amount of grassland decreased 3.6% between the 1950s and 1985 from 18,455 km2 to 17,788 km2. Since 1985, the overall amount of grassland in the lesser prairie-chicken range has increased 11.9% to 19,898 km2 due to implementation of CRP, although the area of grassland decreased between 1994 and 2013 as CRP contracts were not renewed by landowners. Since 1986 grassland in Kansas became more connected and less fragmented in response to the CRP. While the CRP has been successful in increasing grassland quantity and connectivity throughout the lesser prairie-chicken range in Kansas, offsetting loss of grassland since the 1950s, abundance and occupied range of lesser prairie-chickens has declined since the 1980s, suggesting that habitat quality is the principal factor influencing population demography of the species. Although the CRP is contributing to conservation actions for lesser prairie-chickens, efforts to improve habitat quality throughout the range of the lesser prairie-chicken are likely necessary to meet management goals. Continuation of the CRP faces an uncertain future in the face of rising commodity prices, energy development, and reduction in program scope, leaving open the possibility that these areas that have created habitat for lesser prairie-chickens could be lost.

The >2,570,000‐ha Flint Hills ecoregion of Kansas, USA, harbors the largest remaining contiguous tract of tallgrass prairie in North America, a unique system, as the remainder of North America's tallgrass prairie has succumbed to development and conversion. Consequently, the loss and degradation of tallgrass prairie has reduced populations of many North American prairie‐obligate species including the regal fritillary (Speyeria idalia) butterfly. Population abundance and occupied range of regal fritillary have declined >99%, restricting many populations to isolated, remnant patches of tallgrass prairie. Such extensive decline has resulted in consideration of the regal fritillary for protection under the Endangered Species Act. Although it is widely accepted that management practices such as fire, grazing, and haying are necessary to maintain prairie ecosystems, reported responses by regal fritillary to these management regimes have been ambiguous. We tested effects of prescribed fire across short, moderate, and long fire‐return intervals as well as grazing and haying management treatments on regal fritillary density. We also tested the relative influence of habitat characteristics created by these management regimes by measuring density of an obligate host plant (Viola spp.) and canopy cover of woody vegetation, grasses, forbs/ferns, bare ground, and litter. We found density was at least 1.6 times greater in sites burned with a moderate fire‐return interval vs. sites burned with short and long fire‐return intervals. Overall management regardless of fire‐return interval did not have an effect on density. Percent cover of grass had the strongest positive association, while percent cover of woody vegetation had the greatest negative effect on density. Our results indicate that patch‐burning is a viable and perhaps even ideal management strategy for regal fritillary in tallgrass prairie landscapes. Additionally, these results elucidate the importance of fire, particularly when applied at moderate‐return intervals to regal fritillary, and corroborate a growing suite of studies that suggest fire is perhaps not as detrimental to populations of regal fritillary as previously believed.

The magnitude and distribution of lead contamination remain unknown in wetland systems. Anthropogenic deposition of lead may be contributing to negative population-level effects in waterfowl and other organisms that depend on dynamic wetland habitats, particularly if they are unable to detect and differentiate levels of environmental contamination by lead. Detection of lead and behavioral response to elevated lead levels by waterfowl is poorly understood, but necessary to characterize the risk of lead-contaminated habitats. We measured the relationship between lead contamination of wetland soils and habitat use by mottled ducks (Anas fulvigula) on the Upper Texas Coast, USA. Mottled ducks have historically experienced disproportionate negative effects from lead exposure, and exhibit a unique nonmigratory life history that increases risk of exposure when inhabiting contaminated areas. We used spatial interpolation to estimate lead in wetland soils of the Texas Chenier Plain National Wildlife Refuge Complex. Soil lead levels varied across the refuge complex (0.01–1085.51 ppm), but greater lead concentrations frequently corresponded to areas with high densities of transmittered mottled ducks. We used soil lead concentration data and MaxENT species distribution models to quantify relationships among various habitat factors and locations of mottled ducks. Use of habitats with greater lead concentration increased during years of a major disturbance. Because mottled ducks use habitats with high concentrations of lead during periods of stress, have greater risk of exposure following major disturbance to the coastal marsh system, and no innate mechanism for avoiding the threat of lead exposure, we suggest the potential presence of an ecological trap of quality habitat that warrants further quantification at a population scale for mottled ducks.

Use of dogs has increased for multiple wildlife research purposes ranging from carnivore scat detection to estimation of reptile abundance. Use of dogs is not particularly novel for upland gamebird biologists, and pointing dogs have been long considered an important research tool. However, recent advances in Global Positioning System (GPS) technology and the development of hierarchical modeling approaches that account for imperfect detection may improve estimates of occupancy and density of cryptic species such as the American woodcock (Scolopax minor; hereafter, woodcock). We conducted surveys for woodcock using a trained pointing dog wearing a GPS collar during the winters of 2010–2011 and 2011–2012 in East Texas, USA. We surveyed 0.5-km-radius circular plots (n = 24; survey sites) randomly placed along secondary roads in Davy Crockett National Forest and on private timber property. Surveys lasted 1.5 hrs and were repeated 3–5 times each winter. We estimated woodcock occupancy and density using multiple modeling approaches at the survey site and forest stand scales within survey sites. Woodcock occupied 88% (21/24) of survey sites and 48% (39/82) of forest stands (i.e., unique cover types) within sites. Using a modified distance sampling technique, we estimated an average density of 0.16 birds/ha (SE = 0.13) throughout both study areas. We describe the first attempt to blend use of pointing dogs with hierarchical modeling approaches to derive estimates of regional diurnal woodcock occupancy and density, and describe relationships between these estimates of abundance and habitat covariates. Although forest stand occupancy estimates had the lowest coefficients of variation, our estimates of density provided the most useful inference of habitat use. Surveys using pointing dogs paired with hierarchical models of occupancy and density may provide a cost-efficient and effective approach to estimate habitat abundance at broad spatial scales.

Playa wetlands are the primary habitat for numerous wetland-dependent species in the Southern Great Plains of North America. Plant and wildlife populations that inhabit these wetlands are reciprocally linked through the dispersal of individuals, propagules and ultimately genes among local populations. To develop and implement a framework using network models for conceptualizing, representing and analyzing potential biological flows among 48,981 spatially discrete playa wetlands in the Southern Great Plains. We examined changes in connectivity patterns and assessed the relative importance of wetlands to maintaining these patterns by targeting wetlands for removal based on network centrality metrics weighted by estimates of habitat quality and probability of inundation. We identified several distinct, broad-scale sub networks and phase transitions among playa wetlands in the Southern Plains. In particular, for organisms that can disperse >2 km a dense and expansive wetland sub network emerges in the Southern High Plains. This network was characterized by localized, densely connected wetland clusters at link distances (h) >2 km but

Agriculture and development have caused landscape change throughout the southwestern Great Plains in the range of the lesser prairie-chicken (Tympanuchus pallidicinctus). Landscape alteration within the lesser prairie-chicken range may contribute to range contraction and population losses through decreases in survival rates. Our objectives were to determine if: (1) landscape configuration (i.e., the spatial arrangement of habitat) or composition (i.e., the amount of habitat), at the study.site scale, affected annual survival of females, (2) relationships exist between landscape context (i.e., landscape configuration and composition) and weekly survival to assess effects of landscape composition and configuration on lesser prairie-chicken populations, and (3) anthropogenic features influenced daily mortality risk. We captured 170 female lesser prairie-chickens and used very-high-frequency and GPS (Global Positioning System) transmitters to track their movement and survival for 2 y. We used known-fate survival models to test if landscape configuration or composition within three sites in Kansas were related to differences in female survival among sites. In addition we tested for relationships between weekly survival and landscape configuration or composition within home ranges. Finally, we used Andersen-Gill models to test the influence of distance to anthropogenic features on daily mortality risk. Differences in survival were evident between sites with differing landscape compositions as annual survival in Northwestern Kansas (S=0.27) was half that of Clark County, Kansas (S=0.56), which corresponded with 41.9% more grassland on the landscape in Clark County; landscape configuration did not measurably differ among sites. Survival was greater for prairie-chickens with home-ranges that had greater patch richness and in areas with 30% crop and 57% grassland. Female lesser prairie-chickens also experienced greater mortality risk closer to fences at patch edges. Further conversion of grassland landscapes occupied by lesser prairie-chickens should be avoided to reduce habitat loss and fragmentation thresholds that could affect survival. We suggest continued encouragement of Conservation Reserve Program enrollment in western areas of the lesser prairie-chicken range to maintain or increase the amount of grassland to increase annual survival. Federal Aid in Wildlife Restoration Grant [W-73-R]; US Geological SurveyUnited States Geological Survey; US Dept ofAgriculture (USDA), Natural Resources Conservation Service, Lesser Prairie-Chicken Initiative; Kansas Wildlife, Parks, and Tourism [KS W-73-R-3]; USDA Farm Services CRP Monitoring, Assessment, and Evaluation [7, KSCFWRU RWO 62] Wethank J. Pitman, K. Sexton, J. Kramer, M. Mitchener, D. Dahlgren, J. Prendergast, C. Berens, G. Kramos, A. Flanders, and S. Hyberg for their assistance with the project. Research was funded by the Federal Aid in Wildlife Restoration Grant W-73-R; US Geological Survey; US Dept ofAgriculture (USDA), Natural Resources Conservation Service, Lesser Prairie-Chicken Initiative; Kansas Wildlife, Parks, and Tourism (Federal Assistance Grant KS W-73-R-3); USDA Farm Services CRP Monitoring, Assessment, and Evaluation (12-IA-MRE CRP TA#7, KSCFWRU RWO 62). We also thank two anonymous reviewers for reviewing earlier versions of this manuscript. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. Public domain – authored by a U.S. government employee

The Black-necked Stilt (Himantopus mexicanus) is a migratory shorebird of temperate and tropical America. Declining wetland quality and associated declines in hydrological integrity may contribute to widespread habitat loss for stilts nesting on the upper Texas Gulf of Mexico coast of the USA, as both fresh and brackish marshes are converting to open water and saline marsh. Nests (n = 356) were monitored in three wetland types on the upper Texas coast from 21 April-30 June 2011-2012. Of these 356 nests, 151 were located in managed freshwater wetlands (16 in 2011 and 135 in 2012), 128 were located in managed intermediate wetlands (75 in 2011 and 53 in 2012), and 77 were located in rice fields (all in 2012). Collectively, nest success was 0.2% (0 in rice fields and as high as 4.3% in freshwater wetlands in 2012), among the lowest ever reported for the species. The most frequent cause of nest failure was predation by mammalian and avian predators (∼50%). Daily nest survival rate was positively related to mudflat nesting substrates and negatively related to colony size, rice field, and brackish coastal wetland habitats. Future efforts to minimize edge effects in managed wetlands may prove valuable to improve nest success of stilts and other species that nest in similar wetland types.

For millennia grasslands have provided a myriad of ecosystem services and have been coupled with human resource use. The loss of 46% of grasslands worldwide necessitates the need for conservation that is spatially, temporally, and socioeconomically strategic. In the Southern Great Plains of the United States, conversion of native grasslands to cropland, woody encroachment, and establishment of vertical anthropogenic features have made large intact grasslands rare for lesser prairie-chickens (Tympanuchus pallidicinctus). However, it remains unclear how the spatial distribution of grasslands and anthropogenic features constrain populations and influence conservation. We estimated the distribution of lesser prairie-chickens using data from individuals marked with GPS transmitters in Kansas and Colorado, USA, and empirically derived relationships with anthropogenic structure densities and grassland composition. Our model suggested decreased probability of use in 2-km radius (12.6 km(2)) landscapes that had greater than two vertical features, two oil wells, 8 km of county roads, and 0.15 km of major roads or transmission lines. Predicted probability of use was greatest in 5-km radius landscapes that were 77% grassland. Based on our model predictions, similar to 10% of the current expected lesser prairie-chicken distribution was available as habitat. We used our estimated species distribution to provide spatially explicit prescriptions for CRP enrollment and tree removal in locations most likely to benefit lesser prairie-chickens. Spatially incentivized CRP sign up has the potential to provide 4189 km2 of additional habitat and strategic application of tree removal has the potential to restore 1154 km(2). Tree removal and CRP enrollment are conservation tools that can align with landowner goals and are much more likely to be effective on privately owned working lands. Kansas Wildlife, Parks, and Tourism (Federal Assistance Grant) [KS W-73-R-3]; United States Department of Agriculture (USDA) Farm Services CRP Monitoring, Assessment, and Evaluation [12-IA-MRE CRP TA, KSCFWRU RWO 62, 7]; USDA Natural Resources Conservation Service, Lesser Prairie-Chicken Initiative We thank Kent Fricke, three anonymous reviewers, and the associate editor for providing reviews that improved the quality of the manuscript. We thank K. Schultz and A. Chappell for capturing and providing GPS data from lesser prairie-chickens captured on the Cimarron National Grasslands. B. Anderson, S. Baker, S. Bard, G. Brinkman, K. Broadfoot, R. Cooper, J. Danner, J. Decker, E. D. Entsminger, R. M. Galvin, N. Gilbert, A. Godar, G. Gould, B. Hardy, S.P. Hoffman, D. Holt, B. M. Irle, T. Karish, A. Klais, H. Kruckman, K. Kuechle, S. J. Lane, E. A. Leipold, J. Letlebo, E. Mangelinckx, L. McCall, A. Nichter, K. Phillips, J. K. Proescholdt, J. Rabon, T. Reed, A. Rhodes, B. E. Ross, D. Spencer, A. M. Steed, A. E. Swicegood, P. Waldron, B. A. Walter, I. Waters, W. J. White, E. Wiens, J. B. Yantachka, and A. Zarazua, provided much needed assistance with data collection. We greatly appreciate the logistic and technical support provided by J. C. Pitman, J. Kramer, M. Mitchener, D. K. Dahlgren, J. A. Prendergast, C. Berens, G. Kramos, and A. A. Flanders. Funding for the project was provided by Kansas Wildlife, Parks, and Tourism (Federal Assistance Grant KS W-73-R-3); United States Department of Agriculture (USDA) Farm Services CRP Monitoring, Assessment, and Evaluation (12-IA-MRE CRP TA#7, KSCFWRU RWO 62); and USDA Natural Resources Conservation Service, Lesser Prairie-Chicken Initiative. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. Public domain – authored by a U.S. government employee

The lesser prairie-chicken (Tympanuchus pallidicinctus) has experienced declines in population and occupied range since the late 1800s and is currently proposed for Federal protection under the Endangered Species Act. Populations and the distribution of lesser prairie-chickens in Texas, USA, are thought to be at or near all-time lows. Currently, there is a paucity of data on the wintering ecology of the species. We measured home range, habitat use, and survival of lesser prairie-chickens during the non-breeding seasons (1 Sep–28 Feb) of 2008–2009, 2009–2010, and 2010–2011 in sand shinnery oak (Quercus havardii) landscapes in the West Texas panhandle region. Home range size did not differ among years or between females (503 ha) and males (489 ha). Over 97% of locations of both male and female prairie-chickens were within 3.2 km of the lek of capture, and 99.9% were within 3.2 km of an available water source (i.e., livestock water tank). Habitat cover types were not used proportional to occurrence within the home ranges; grassland-dominated areas with co-occurring sand shinnery oak were used more than available, but sand sagebrush (Artemisia filifolia)-dominated areas with grassland and sand sagebrush-dominated areas with bare ground were both used less than available. Survival rates during the first 2 non-breeding seasons (>80%) were among the highest reported for the species. However, survival during the third non-breeding season was only 57%, resulting in a 3-year average of 72%. It does not appear that non-breeding season mortality is a strong limiting factor in lesser prairie-chicken persistence in the study area. © 2013 The Wildlife Society.

We quantified changes in long-term Snowy Plover (Charadrius nivosus) survey counts and return rates, estimated current sex and age-specific apparent survival and encounter rates, and calculated recruitment thresholds needed to maintain a stable population in the Southern High Plains of Texas. Mean survey counts of adult Snowy Plovers decreased by 78% at one saline lake (from 80 adults/survey to 18 adults/survey) from 1999–2000 to 2008–2010 but remained consistent at an alternate lake (from 45 adults/survey to 41 adults/survey). Adult and juvenile return rates have similarly declined within this time frame by 25 and 62%, respectively. Long-term declines in return rates may be the result of increased mortality from declining habitat conditions either within or outside the breeding season. Current estimates of adult (65%) and juvenile (12%) apparent survival are lower than most other estimates for Snowy Plovers throughout their range. Current estimates of adult and juvenile apparent survival and ret...

Playas are the primary wetland system in the Southern Great Plains (SGP) of North America providing critical stopover habitats for migratory birds in the Western Hemisphere. Collectively, these wetlands form the keystone ecosystem in this region supporting biodiversity for North America and provide habitat for native plants and animals that are essential for maintenance of international biological diversity along with other local and regional ecological services. Large-scale landscape changes in this region, primarily as a result of agriculturally-based anthropogenic impacts, threaten playas with functional loss and physical extinction. These impacts are not considered in current estimates of the number of extant functional playas, leading to biased estimates by groups or agencies extrapolating research or monitoring results, conducting conservation planning, and modeling impacts of future climate change. Using a combination of stochastic and empirical data, we identified impacts to playas and playa watersheds and quantified the extent and rate of these impacts relative to physical loss. Only 0.2% of playas had no wetland or watershed modification and we conservatively estimate that 17% of playas recently existing on the landscape no longer are represented by an apparent depression. With the inclusion of sediment volume estimates where ≥100% of the volume of original differentiated playa soil has been filled, 60% or 16,855 playas have been physically lost from the SGP despite a continued presence of a depression. Data also show that small playas are being lost more rapidly than larger ones; the average size (±SE) of extant playas has increased from 7.5 ± 0.47 to 8.5 ± 0.55 ha. The reduced ecological condition of 95.3% of playas remaining on the landscape exceeds historical predictions of a maximum 85% of playas ever being modified. Given the results of this study these changes can now be accounted for, resulting in more informed management and conservation of playas and bring awareness to the urgency of implementation of effective conservation measures for playas and the species that depend on this keystone ecosystem.

Snowy plover (Charadrius nivosus) populations have declined throughout their range, in part because of habitat degradation and poor nest success, making information regarding regionally specific nest site selection and spatial patterns important when considering habitat conservation and management guidelines. We determined nest site selection characteristics (n = 180) and examined spatial patterns (n = 215) of snowy plover nests in saline lakes in the Southern High Plains (SHP) of Texas. At 104 nests, we examined the influence of substrate type on nest temperatures and heat mitigation. Snowy plover nests were more likely to be found near an object, on pebble substrate, and with fewer plants than random sites. High use areas were generally located in areas with pebble substrate and on human-made or natural islands, berms, and peninsulas. Overall, nests placed on pebble substrate had lower temperatures during the day than nests placed on sand substrates. Nest placement on pebble substrate may be valuable to nesting snowy plovers, providing thermal advantages to incubating adults and depressing potentially high nest predation rates. Management guidelines for this region should emphasize the importance of addressing key elements of snowy plover nesting habitat including the presence of pebble substrate and reducing vegetation encroachment. © 2012 The Wildlife Society.

Playa wetlands (shallow, circular, depressional wetlands) in the Southern Great Plains are essential to the maintenance of regional biodiversity. However, the relative importance of habitat characteristics for maintaining avian species richness and density in playas is unknown. We examined influences of local- and landscape-scale characteristics on avian communities in wet playas. We conducted biweekly avian surveys on 80 playas (40 playas/year) from summer 2003 through spring 2005. Avian species richness was positively related to playa area and hydroperiod. More avian species and greater densities of birds were found at wetlands when water depths were intermediate (30–80 cm) during fall, winter and spring. Increased cultivation in playa watersheds increased total-, native-, and exotic-avian species richness. However, unsustainable sediment accumulation caused by cultivation can fill the playa basin and result in the loss of playa function, which will not benefit future bird populations. Landscape variables generally were less consistent with avian communities among seasons compared to local variables. Playas with greater numbers of other playas within 1 and 10 km had lower bird densities than those with fewer surrounding playas, likely due to the increase of habitat availability. Larger playas within predominantly uncultivated watersheds, that have an intermediate water depth, longer hydroperiod (within a season), and 26–50% vegetation cover are likely to maximize seasonal native avian richness. However, manipulating static water depths and maintaining long hydroperiods over extended periods will not meet avian objectives over the long term because playas must go through natural wet/dry fluctuations between years to maintain the desired plant community and productivity that supports diverse avian populations.

We developed and evaluated the performance of a metapopulation model enabling managers to examine, for the first time, the consequences of alternative management strategies involving habitat conditions and hunting on both harvest opportunity and carrying capacity (i.e., equilibrium population size in the absence of harvest) for migratory waterfowl at a continental scale. Our focus is on the northern pintail (Anas acuta; hereafter, pintail), which serves as a useful model species to examine the potential for integrating waterfowl harvest and habitat management in North America. We developed submodel structure capturing important processes for pintail populations during breeding, fall migration, winter, and spring migration while encompassing spatial structure representing three core breeding areas and two core nonbreeding areas. A number of continental-scale predictions from our baseline parameterization (e.g., carrying capacity of 5.5 million, equilibrium population size of 2.9 million and harvest rate of 12% at maximum sustained yield [MSY]) were within 10% of those from the pintail harvest strategy under current use by the U.S. Fish and Wildlife Service. To begin investigating the interaction of harvest and habitat management, we examined equilibrium population conditions for pintail at the continental scale across a range of harvest rates while perturbing model parameters to represent: (1) a 10% increase in breeding habitat quality in the Prairie Pothole population (PR); and (2) a 10% increase in nonbreeding habitat quantity along in the Gulf Coast (GC). Based on our model and analysis, a greater increase in carrying capacity and sustainable harvest was seen when increasing a proxy for habitat quality in the Prairie Pothole population. This finding and underlying assumptions must be critically evaluated, however, before specific management recommendations can be made. To make such recommendations, we require (1) extended, refined submodels with additional parameters linking influences of habitat management and environmental conditions to key life-history parameters; (2) a formal sensitivity analysis of the revised model; (3) an integrated population model that incorporates empirical data for estimating key vital rates; and (4) cost estimates for changing these additional parameters through habitat management efforts. We foresee great utility in using an integrated modeling approach to predict habitat and harvest management influences on continental-scale population responses while explicitly considering putative effects of climate change. Such a model could be readily adapted for management of many habitat-limited species.

Lesser prairie-chickens (Tympanuchus pallidicinctus) are traditionally monitored by spring road- based lek surveys and counts of males attending leks. Several weaknesses exist with ground-based monitoring methods such as the bias of restricting surveys to roads, unknown probability of lek detection, and man-hours required to survey large tracts of habitat. We evaluated aerial surveys to locate lesser prairie-chicken leks in Texas and New Mexico using a Cessna 172 airplane (C172), R-22 Beta II helicopter (R-22), and R-44 Raven II helicopter (R-44) during spring 2007-2008. We determined lek activity during surveys with remote cameras placed on leks and cross-referenced time on the photo frame to time on our Global Positioning System flight log. From remote cameras we found that 305 leks were available for detection during survey flights. We determined lek detectability was 32.7% (95% CI ¼ 20.3-47.1%) in the C172, 72.3% (64.50- 79.14%) in the R-22, and 89.8% (82.0-95.0%) in the R-44. We created 16 a priori logistic regression models incorporating aircraft platform, distance to lek, survey date, lek size, and lek type to explain lek detection from aerial surveys. Our top ranked model included platform, distance, and lek type (model weight; wi ¼ 0.288). We had four competitive models and model averaged to draw inferences. Model averaging showed that detectability was generally greatest with the R-44, followed by the R-22, and lowest with the C172, with a slight deviation from this ranking at increased distances. Within our transect width, model averaging also suggested that detectability decreased as distance from the transect to the lek increased during helicopter surveys, and detectability increased as distance from the transect to the lek increased during C172 surveys. Furthermore, man-made leks were more likely to be detected than natural leks and large leks were more likely to be detected than medium or small leks. Aerial surveys effectively locate new leks and monitor lek density, and alleviate weaknesses associated with ground-based monitoring. We recommend using the R-44 to conduct lek surveys while flying at an altitude of 15 m at a speed of 60 km/hr on sunny mornings. 2011 The Wildlife Society.

Mottled ducks Anas fulvigula are nonmigratory and primarily depend on habitats associated with coastal marshes of the Gulf Coast for their entire life cycle. Much of remaining available coastal marsh habitats of the Texas Gulf Coast are managed by state and federal agencies. The Texas Gulf Coast breeding population of mottled ducks has recently declined dramatically. To aid in development of management plans for breeding mottled ducks, we estimated the number of potential pair ponds available on the Texas Chenier Plain National Wildlife Refuge Complex relative to marsh type (fresh, intermediate, brackish, and saline), estimated occupancy, compared habitats of used and unused pair ponds, and evaluated pair pond selection relative to marsh type during spring 2004 and 2005. Greater than 18,000 ponds were recorded in >22,400 ha of coastal marsh with >74% of potential pair ponds in the intermediate marsh type. Average pond size of 425 randomly selected ponds across marsh types was 0.09 ha. Pond occupancy was estimated as 1.3% and 2.5% in 2004 and 2005, respectively, in 634 ponds surveyed for presence of mottled duck pairs. Mottled ducks selected for ponds in fresh marsh with short surrounding vegetation associated with recent cattle grazing, but avoided recently (

Long-billed Curlew (Numenius americanus) populations have declined during the past 150 years in part due to destruction and fragmentation of grasslands used during the breeding season. Here, multiscale habitat characteristics best predicting number of Long-billed Curlews, detected during range-wide surveys conducted throughout the United States in 2004 and 2005, were determined. Long-billed Curlews were most often observed in habitats classified primarily as grassland habitat and secondarily as shortgrass or pasture/rangeland, all with low vegetation heights (i.e. 4–15 cm). Numbers of Long-billed Curlews were positively associated with wetland habitats on a local scale and hay/pasture areas on a landscape scale, but negatively associated with shrub/scrub on local and landscape scales and evergreen forests on a landscape scale. The study confirmed the importance of grassland, cropland, pasture and wetland habitats for breeding Long-billed Curlews across its geographic range in the United States. ...

Unlike other North American prairie-nesting dabbling ducks, northern pintail (Anas acuta) populations have not increased since the early 1990s and remain well below the long-term average for traditional survey areas. Previously reported estimates of annual survival and recovery rates for pintails did not investigate any spatial or temporal factors to explain annual variation of these rates. We used band- recovery data from 1970 to 2003 to test the influence of temporal periods defined by differing harvest regulations and habitat conditions of breeding grounds with spatially delineated regions on survival and recovery rates of northern pintails in North America. We separated regions based on a multiresponse permutation procedure to identify banding blocks with dissimilar recovery distributions based on a cluster analysis. We categorized time by grouping years into temporal periods based on bag limits, season lengths, or overflight versus nonoverflight years. We used the Brownie approach in Program MARK to evaluate 46 a priori models estimating survival and recovery rates. The best approximating model indicated that survival varied with age, sex, and region with additive time and interactive time-by-age and time-by-region effects. Recovery rate was best represented by a fully interactive term comprised of age, sex, region, and year. There were no statistical differences among average annual survival point estimates between age and sex classes within each region, and our estimates were similar to previous unpublished studies. We found the eastern region had decreased survival and increased recovery rates compared to other regions. Trends in pintail survival suggest that variation in annual survival was not the cause of the initial decrease in the northern pintail population and is unlikely the dominant factor preventing the population from increasing. The influence of other population parameters, such as recruitment rate, should be investigated to further evaluate other causes for the population status of northern pintails. Use of the top-ranked model to estimate annual survival and recovery rates for northern pintails in North America, which indicated that annually varying estimates of survival rates were better supported by the data than grouping years into temporal classes (i.e., based on bag limits, season lengths, and overflight yr) can be used by managers and policy makers when considering annual harvest regulations and effects of conservation efforts. Managers should incorporate these estimates into future demographic studies of pintails as well as consider using the top-ranked model for future analyses of band-recovery data.

Sand shinnery oak (Quercus havardii) communities, a major component of grassland habitat of birds in eastern New Mexico, frequently are managed with livestock grazing and herbicide application for control of shrubs. We examined nest density, nest-site selection, and daily rate of survival of nests of grassland birds among four combinations of treatments with tebuthiuron (0.75 kg/ha) and a short-duration, rotational-grazing system being used to restore sand shinnery oak communities in eastern New Mexico. During breeding seasons 2004 and 2005, we searched 4-ha subplots in four tebuthiuron-grazing-combination replicates for nests, measured vertical and overhead cover at each nest site and an associated random point, and estimated daily rate of survival of nests using program MARK. Density of nests was similar among all treatments but greater in 2005 than 2004. Although vertical cover differed among treatments and between years, it did not affect selection or success of nest sites. Overhead cover als...

We assessed the effects of capture period, capture subregion, age, sex and habitat conditions on the body condition of migrating and wintering Northern Pintails (Anas acuta) in the Playa Lakes Region (PLR) of northwestern Texas. Body condition varied with sex, age, subregion and month during the wet 2002. During 2002, after-hatch-year (AHY) males had more carcass fat (g and percent) than did AHY females. Likewise, AHY males had 34% more carcass fat (g) than hatch-year (HY) males, but we did not detect differences between AHY and HY females. The average increase in carcass fat (g) from October to November-December during wet 2002, was 26% for males and 42 to 93% (HY) for females. Body condition of pintails in the south exceeded those captured in northern subregions of the PLR during wet 2002, but not dry 2003. Southern caught AHY males had 31% more carcass fat (g) than northern caught AHY males, but we found no differences for HY males. Females caught in southern subregions also had 24–77% more c...

Overwinter body condition (e.g., fat) provides an index to the health of northern pintail (Anas acuta) populations and may be a factor in the decline of the continental pintail population that has been previously overlooked or understated. We compared body condition between 1984–1985 and 2002–2003, and found that body condition of pintails arriving during early winter in the Playa Lakes Region (PLR) of Texas, USA, has declined by an average of 32%. Body fat levels declined at varying levels with juvenile males showing the largest decline of 41%, followed by adult females with 39%, juvenile females with 30%, and adult males with an overall 18% decline. Declines are likely related to declines in migration and wintering habitat quantity and quality within the PLR and potentially across the Central Flyway. We recommend further acquisition and management of playas to ensure that these valuable habitats remain available to provide critical habitats for migrating and wintering pintails and other waterfo...

Scolopax minor (American Woodcock) populations have been declining for the past several decades. Weather conditions have been hypothesized to affect reproductive efforts in February and March in the southern United States, but similar influences on January breeding activities are unknown. We used a 17-year harvest data set from eastern Texas to examine the influence of several temperature and precipitation measures, female body mass, and forest habitat type on the occurrence of reproduction by Woodcock in January. Only adult females exhibited characteris- tics of breeding activity. There was annual variation (0-29%) in the occurrence of breeding adult females, but local temperature and precipitation measures were not correlated with January reproduction. More breeding Woodcock were harvested in open sapling stands than other habitats. Environmental factors other than monthly temperature and precipitation may influence January reproductive efforts of Wood- cock in eastern Texas. We suggest investigation of timing of fall migration and condition of the birds upon arrival on wintering grounds as potential factors influenc- ing reproductive efforts in January.

Playa wetlands provide functions critical to the existence of life on the High Plains portion of the Great Plains, including surface drainage, aquifer recharge, and wildlife habitat. These small, circular, isolated depressional wetlands with closed watersheds have a dynamic, unpredictable hydroperiod, which is essential to the maintenance of biodiversity. Most numerous in the Southern High Plains of northwestern Texas and eastern New Mexico, playas have been impacted by sedimentation, pit excavation, road construction, industrial and municipal wastewater, feedlot runoff, urban development, overgrazing, and deliberate filling. Despite being declared, as a wetland class, jurisdictional “waters of the United States” since 1977, regulations and laws for conservation of wetland functions have seldom been applied to playas. The January 2001 Supreme Court decision, Solid Waste Agency of Northern Cook County (SWANCC) v. United States Army of Corps of Engineers, likely eliminated federal regulation of impacts covered by the Clean Water Act in all but a few playas. Although still subject to the Federal “Swampbuster” provision enacted by the 1985 Food Security Act, extended natural dry periods allows for frequent cultivation and other activities in playas without incurring violation, contributing to the continued degradation of playa functions. None of the states with significant numbers of playas have regulations for the conservation of playa functions. Suggestions for the successful future conservation of playas and their associated functions include (1) increased promotion and implementation of existing federal and state conservation programs specifically for playas; (2) proposed state regulations for playa conservation; (3) recognition of agricultural impacts on wetland determinations; (4) creation of Wetland Management Districts to preserve intact, functioning playas; and (5) increased public education on the value of playas.

The cloacal cestode Cloacotaenia megalops is one of the most common helminths of waterfowl. We investigated the effect of this parasite on the body condition of wintering waterfowl populations and compared prevalence among age-sex classes, over time and between habitat types on the upper Gulf Coast of Texas (USA) from October 1986-February 2000. Greater than 9,500 birds of 25 waterfowl species were examined for the parasite. There was no statistical difference (P0.05) in body condition between birds with and without the parasite. Average prevalence was lowest for geese (mean = 3.7%) versus 21 to 71% in duck species. Average prevalence was similar (P = 0.81) between diving ducks (mean = 46.9%) and puddle ducks (mean = 43.9%). Prevalence varied among age-sex classes and was related to sex rather than age. Variation among age-sex classes suggests differences in diet between sexes of duck species on the wintering grounds. There was no evidence for declining prevalence over the wintering period. Prevalence differed (P0.05) between collection sites, and thereby habitat types, for several species. Temporal trends indicate stable prevalence of C. megalops for diving ducks and increasing prevalence for puddle ducks. The increasing trend for puddle ducks may indicate declining habitat conditions resulting in increased exposure to the intermediate ostracod host.

There are 25,000-30,000 playa wetlands in the intensively cultivated Southern Great Plains of the United States. Knowledge of area and watershed influences on wetland flora are needed to guide their conser- vation. We surveyed plant-community composition in 224 playas over 360,000 km 2 and examined the rela- tionships of species richness and diversity (Shannon's) to playa area and watershed disturbance. The rela- tionship of increasing species diversity to increasing area is most often hypothesized to be associated with an increasing number of habitats and/or larger populations as area increases. Watershed disturbance ( peren- nial grassland vs. annual cropland) was included to determine its relationship to floral diversity and bioin- vasion by exotic species. For all (terrestrial and wetland) plant species and all playas, there were only mar- ginal relationships ( r 2 � 0.1) between area and richness and diversity. Analyses supported the hypothesis that the number of habitats affects playa plant diversity because number of habitats changed little as playa area increased. Tests on only wetland plant species suggested stronger ( r 2 � 0.2) relationships between rich- ness and area. This relationship also was not likely related to increased population size, because large playas remain flooded longer than small playas, and therefore wetland plant species have a greater opportunity to become established. Finally, playas with cropland watersheds had more ( p � 0.05) exotic species, higher di- versity, and fewer perennial species than playas with grassland watersheds. Because watershed cultivation has altered playa hydroperiod and increased frequency of disturbance, playas are associated with a flora dominated more by annuals and exotics. Conservation efforts aimed at preserving playa wetland plant diver- sity and native communities should focus not only on the area of the wetland but also on the condition of its watershed.

We describe the landscape of the Southern High Plains, including the important remaining native feature of the area; playa wetlands. Playas are ephemeral wetlands on the prairie occupying approximately 2% of the landscape. Playas re-charge the Ogallala aquifer, are incorporated in farming irrigation systems, and represent the predominant wildlife habitat of the area. The importance of playas to mammalian, avian, other vertebrate, and floral biodiversity is detailed. Potential threats to playas are described and include chemical contamination, physical modication, and grazing. Recommended avenues for conservation of playas include nationwide recognition of the importance of playas, increased emphasis on basic research, and development of management plans for landscape biodiversity.

Great Plains toads (Bufo cognatus), plains spadefoots (Spea bombifrons), and New Mexico spadefoots (S. multiplicata) commonly breed in playa wetlands on the Southern High Plains of northwest Texas and eastern New Mexico. Great Plains toads (n 129) consumed 43 invertebrate taxa, plains spadefoots (n = 24) consumed 12 invertebrate taxa, and New Mexico spadefoots (n = 38) consumed 20 invertebrate taxa during 1995 and 1996. Diet overlap was greatest between Great Plains toads and New Mexico spadefoots during 1995. Carabids were the most commonly consumed food item by all species during 1995 and 1996. Conservation of anurans that use playas should focus on playas that are surrounded by terrestrial grassland habitats that provide foraging sites for breeding anurans.

Grasslands are among the most imperiled ecosystems in North America. Reasons that grasslands are threatened include conversion to row-crop agriculture, fragmentation, and changes in fire regimes. The reduction of fire processes in remaining prairies has resulted in tree encroachment and establishment in grasslands, further reducing grassland quantity and quality. Grassland birds have been experiencing precipitous population declines in recent decades, commensurate with landscape changes to grasslands. The lesser prairie-chicken (Tympanuchus pallidicinctus Ridgway) is a declining species of prairie grouse of conservation concern. We used second-and third-order habitat selection metrics to test if female lesser prairie-chickens avoid grasslands where trees were present. Our results indicated that female lesser prairie-chickens selected habitats avoiding the nearest trees by 283 m on average, nearly twice as far as would be expected at random. Lesser prairie-chickens were 40 times more likely to use habitats with tree densities of 0 trees.ha(-1) than habitats with 5 trees.ha(-1). Probability of use indicated that lesser prairie-chickens were 19 times more likely to use habitats 1000 m from the nearest tree when compared with using habitats 0 m from the nearest tree. Nest survival was not affected at densities 2 trees.ha(-1). Avoidance of trees could be due to perceived increased predation risk, reduced habitat quality, or a combination of these potentially confounding factors. Preventing further establishment and expansion of trees in landscapes occupied by lesser prairie-chickens could contribute to the continued persistence of the species. Additionally, restoring grasslands through tree removal may facilitate conservation efforts for grassland species such as the lesser prairie-chicken by improving habitat quality and promoting expansion of occupied range. (C) 2017 The Society for Range Management. Published by Elsevier Inc. All rights reserved. Federal Aid in Wildlife Restoration Grant [W-73-R]; US Geological Survey; USDept of Agriculture (USDA), Natural Resources Conservation Service, Lesser Prairie-Chicken Initiative; Kansas Wildlife, Parks, and Tourism (Federal Assistance Grant) [KS W-73-R-3]; USDA Farm Services CRP Monitoring, Assessment, and Evaluation [7, KSCFWRU RWO 62] Research was funded by the Federal Aid in Wildlife Restoration Grant W-73-R; US Geological Survey; USDept of Agriculture (USDA), Natural Resources Conservation Service, Lesser Prairie-Chicken Initiative; Kansas Wildlife, Parks, and Tourism (Federal Assistance Grant KS W-73-R-3); USDA Farm Services CRP Monitoring, Assessment, and Evaluation (12-IA-MRE CRP TA#7, KSCFWRU RWO 62). Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the US Government. At the time of research, Pitman was the Small Game Program Coordinator, Kansas Dept of Wildlife, Parks, and Tourism, Emporia, KS 66801.