1. Evaluation of a recombinant measles virus expressing hepatitis C virus envelope proteins by infection of human PBL-NOD/Scid/Jak3null mouse
- Author
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Satoh, Masaaki, Saito, Makoto, Tanaka, Kohsuke, Iwanaga, Sumako, Ali, Salem Nagla Elwy Salem, Seki, Takahiro, Okada, Seiji, Kohara, Michinori, Harada, Shinji, Kai, Chieko, and Tsukiyama-Kohara, Kyoko
- Subjects
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MEASLES virus , *HEPATITIS C virus , *PROTEINS , *INFECTION , *LEUKOCYTES , *IMMUNOGENETICS , *VACCINES , *LABORATORY mice - Abstract
Abstract: In this study, we infected NOD/Scid/Jak3null mice engrafted human peripheral blood leukocytes (hu-PBL-NOJ) with measles virus Edmonston B strain (MV-Edm) expressing hepatitis C virus (HCV) envelope proteins (rMV-E1E2) to evaluate the immunogenicity as a vaccine candidate. Although human leukocytes could be isolated from the spleen of mock-infected mice during the 2-weeks experiment, the proportion of engrafted human leukocytes in mice infected with MV (103–105 pfu) or rMV-E1E2 (104 pfu) was decreased. Viral infection of the splenocytes was confirmed by the development of cytopathic effects (CPEs) in co-cultures of splenocytes and B95a cells and verified using RT-PCR. Finally, human antibodies against MV were more frequently observed than E2-specific antibodies in serum from mice infected with a low dose of virus (MV, 100–101 pfu, and rMV-E1E2, 101–102 pfu). These results showed the possibility of hu-PBL-NOJ mice for the evaluation of the immunogenicity of viral proteins. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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