1. Distribution and dynamic changes of Huanglongbing pathogen in its insect vector Diaphorina citri .
- Author
-
Guo CF, Kong WZ, Mukangango M, Hu YW, Liu YT, Sang W, and Qiu BL
- Subjects
- Animals, Rhizobiaceae genetics, Rhizobiaceae physiology, Real-Time Polymerase Chain Reaction, Salivary Glands microbiology, Hemolymph microbiology, Hemiptera microbiology, Insect Vectors microbiology, Plant Diseases microbiology, Nymph microbiology, Citrus microbiology, In Situ Hybridization, Fluorescence
- Abstract
The Asian citrus psyllid (ACP) Diaphorina citri Kuwayama is the leading vector of Candidatus Liberibacter asiaticus ( C Las), the causative agent of citrus Huanglongbing (HLB) disease. The distribution and dynamics of C Las within ACP are critical to understanding how the transmission, spread and infection of C Las occurs within its host vector in nature. In this study, the distribution and titer changes of C Las in various tissues of ACP 5
th instar nymphs and adults were examined by fluorescence in situ hybridization (FISH) and real-time quantitative PCR (qPCR) techniques. Results demonstrated that 100% of ACP 5th instar nymphs and adults were infected with C Las following feeding on infected plants, and that C Las had widespread distribution in most of the tissues of ACP. The titers of C Las within the midgut, salivary glands and hemolymph tissues were the highest in both 5th instar nymphs and adults. When compared with adults, the titers of C Las in these three tissues of 5th instar nymphs were significantly higher, while in the mycetome, ovary and testes they were significantly lower than those of adults. FISH visualization further confirmed these findings. Dynamic analysis of C Las demonstrated that it was present across all the developmental ages of ACP adults. There was a discernible upward trend in the presence of C Las with advancing age in most tissues of ACP adults, including the midgut, hemolymph, salivary glands, foot, head, cuticula and muscle. Our findings have significant implications for the comprehensive understanding of the transmission, dissemination and infestation of C Las, which is of much importance for developing novel strategies to halt the spread of C Las, and therefore contribute to the efficient prevention and control of HLB., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision., (Copyright © 2024 Guo, Kong, Mukangango, Hu, Liu, Sang and Qiu.)- Published
- 2024
- Full Text
- View/download PDF