13 results on '"Li, Liyan"'
Search Results
2. The histone demethylase JMJD1C regulates CPS1 expression and promotes the proliferation of paroxysmal nocturnal haemoglobinuria clones through cell metabolic reprogramming.
- Author
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Chen Y, Liu H, Wang C, Chen W, Li L, Wu J, Wang G, Ling GS, and Fu R
- Subjects
- Humans, Animals, Mice, K562 Cells, Male, Female, Apoptosis, Metabolic Reprogramming, Oxidoreductases, N-Demethylating, Jumonji Domain-Containing Histone Demethylases genetics, Jumonji Domain-Containing Histone Demethylases metabolism, Cell Proliferation, Hemoglobinuria, Paroxysmal pathology, Hemoglobinuria, Paroxysmal genetics, Hemoglobinuria, Paroxysmal metabolism
- Abstract
Paroxysmal nocturnal haemoglobinuria (PNH) is a disorder resulting from erythrocyte membrane deficiencies caused by PIG-A gene mutations. While current treatments alleviate symptoms, they fail to address the underlying cause of the disease-the pathogenic PNH clones. In this study, we found that the expression of carbamoyl phosphate synthetase 1 (CPS1) was downregulated in PNH clones, and the level of CPS1 was negatively correlated with the proportion of PNH clones. Using PIG-A knockout K562 (K562 KO) cells, we demonstrated that CPS1 knockdown increased cell proliferation and altered cell metabolism, suggesting that CPS1 participates in PNH clonal proliferation through metabolic reprogramming. Furthermore, we observed an increase in the expression levels of the histone demethylase JMJD1C in PNH clones, and JMJD1C expression was negatively correlated with CPS1 expression. Knocking down JMJD1C in K562 KO cells upregulated CPS1 and H3K36me3 expression, decreased cell proliferation and increased cell apoptosis. Chromatin immunoprecipitation analysis further demonstrated that H3K36me3 regulated CPS1 expression. Finally, we demonstrated that histone demethylase inhibitor JIB-04 can suppressed K562 KO cell proliferation and reduced the proportion of PNH clones in PNH mice. In conclusion, aberrant regulation of the JMJD1C-H3K36me3-CPS1 axis contributes to PNH clonal proliferation. Targeting JMJD1C with a specific inhibitor unveils a potential strategy for treating PNH patients., (© 2024 British Society for Haematology and John Wiley & Sons Ltd.)
- Published
- 2024
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3. Abnormal expression of CUX1 influences autophagy activation in paroxysmal nocturnal hemoglobinuria.
- Author
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Wu J, Li L, Liu Z, Wang H, Chen Y, Zeng L, Wang G, Liu H, and Fu R
- Subjects
- Humans, Male, Female, K562 Cells, Middle Aged, Adult, Cell Proliferation, Proto-Oncogene Proteins c-akt metabolism, Signal Transduction, TOR Serine-Threonine Kinases metabolism, Phosphatidylinositol 3-Kinases metabolism, Autophagy-Related Protein-1 Homolog metabolism, Autophagy-Related Protein-1 Homolog genetics, Autophagy, Hemoglobinuria, Paroxysmal genetics, Hemoglobinuria, Paroxysmal pathology, Hemoglobinuria, Paroxysmal metabolism, Homeodomain Proteins genetics, Homeodomain Proteins metabolism, Repressor Proteins genetics, Repressor Proteins metabolism, Transcription Factors, Intracellular Signaling Peptides and Proteins
- Abstract
The mechanism underlying autophagy in paroxysmal nocturnal hemoglobinuria (PNH) remains largely unknown. We previously sequenced the entire genome exon of the CD59- cells from 13 patients with PNH and found genes such as CUX1 encoding Cut-like homeobox 1. Peripheral blood samples from 9 patients with PNH and 7 healthy control subjects were obtained to measure CUX1 expression. The correlation between CUX1 messenger RNA expression and PNH clinical indicators was analyzed. To simulate CUX1 expression in patients with PNH, we generated a panel of PNH cell lines by knocking out PIGA in K562 cell lines and transfected lentivirus with CUX1. CCK-8 and EDU assay assessed cell proliferation. Western blotting was used to detect Beclin-1, LC3A, LC3B, ULK1, PI3K, AKT, p-AKT, mTOR, and p-mTOR protein levels. Autophagosomes were observed with transmission electron microscopy. Chloroquine was used to observe CUX1 expression in PNH after autophagy inhibition. Leukocytes from patients with PNH had lower levels of CUX1 messenger RNA expression and protein content than healthy control subjects. The lactose dehydrogenase level and the percentage of PNH clones were negatively correlated with CUX1 relative expression. We reduced CUX1 expression in a PIGA knockout K562 cell line, leading to increased cell proliferation. Levels of autophagy markers Beclin-1, LC3B, LC3A, and ULK1 increased, and autophagosomes increased. Furthermore, PI3K/AKT/mTOR protein phosphorylation levels were lower. CUX1 expression did not change and cell proliferation decreased in CUX1 knocked down PNH cells after inhibition of autophagy by chloroquine. In brief, CUX1 loss-of-function mutation resulted in stronger autophagy in PNH., Competing Interests: Conflict of interest statement. The authors declare that they have no conflicts of interest., (© The Author(s) 2024. Published by Oxford University Press on behalf of Society for Leukocyte Biology.)
- Published
- 2024
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4. The immunologic abnormalities in patients with paroxysmal nocturnal hemoglobinuria are associated with disease progression.
- Author
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Wang G, Che M, Zeng L, Liu H, Li L, Liu Z, and Fu R
- Subjects
- Humans, Male, Female, Adult, Middle Aged, Retrospective Studies, L-Lactate Dehydrogenase blood, Monocytes immunology, Dendritic Cells immunology, CD3 Complex metabolism, Case-Control Studies, Glycosylphosphatidylinositols immunology, Young Adult, Antigens, CD19, Hemoglobinuria, Paroxysmal immunology, Hemoglobinuria, Paroxysmal blood, Disease Progression
- Abstract
Objectives: To suggest the presence of a hyperimmune state in patients, and indicate that immune system attack on glycosylphosphatidylinositol (+) (GPI
+ ) cells while escaping GPI- cell immunity., Methods: We retrospective the immune cell subtypes in peripheral blood from 25 patients visiting Tianjin Medical University General Hospital, Tianjin, China, with classical paroxysmal nocturnal hemoglobinuria (PNH) and 50 healthy controls., Results: The total CD3+ and CD3+ CD8+ cell levels were higher in patients with PNH. The CD3+ cells are positively, correlated with lactate dehydrogenase (LDH; r=0.5453, p =0.0040), indirect bilirubin (r=0.4260, p =0.0379) and Flear- cells in monocytes (r=0.4099, p =0.0303). However, a negative correlation was observed between CD3+ cells and hemoglobin (r= -0.4530, p =0.0105). The total CD19+ cells decreased in patients, and CD19+ cells were negatively correlated with LDH (r= -0.5640, p =0.0077) and Flear- cells in monocytes (r= -0.4432, p =0.0341). Patients showed an increased proportion of total dendritic cells (DCs), with a higher proportion of myeloid DCs (mDCs) within the DC population. Moreover, the proportion of mDC/DC was positively correlated with CD59- cells (II + III types) in red cells (r=0.7941, p =0.0004), Flear- cells in granulocytes (r=0.5357, p =0.0396), and monocytes (r=0.6445, p =0.0095)., Conclusion: Our results demonstrated that immune abnormalities are associated with PNH development., (Copyright: © Saudi Medical Journal.)- Published
- 2024
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5. Long noncoding RNA FAM157C contributes to clonal proliferation in paroxysmal nocturnal hemoglobinuria.
- Author
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Wang H, Liu H, Li L, Chen Y, Liu Z, Li L, Ding S, Ding K, and Fu R
- Subjects
- Humans, Hematopoietic Stem Cells metabolism, Clone Cells chemistry, CD59 Antigens analysis, CD59 Antigens metabolism, Cell Proliferation genetics, RNA, Messenger genetics, RNA, Messenger metabolism, Hemoglobinuria, Paroxysmal genetics, Hemoglobinuria, Paroxysmal diagnosis, RNA, Long Noncoding genetics
- Abstract
Paroxysmal nocturnal hemoglobinuria (PNH) is a rare clonal disease of hematopoietic stem cells (HSCs). Long noncoding RNAs (lncRNAs) perform a wide range of biological functions, including the regulation of gene expression, cell differentiation, and proliferation, but their role in PNH remains unclear.CD59
- and CD59+ granulocytes and monocytes from 35 PNH patients were sorted. High-throughput sequencing was analyzed in 5 PNH patients, and differentially expressed lncRNAs and mRNAs were identified. The mRNAs with fragments per kilobase of exon model per million mapped fragments (FPKM) > 10 in at least 3 patients were selected, and experiments were performed to identify their upstream regulatory lncRNAs. The expression of selected mRNAs and lncRNAs was verified by qRT‒PCR, and the correlation of these expression patterns with clinical data from other 30 PNH patients was analyzed. Then, the functions of the lncRNAs were studied in the PIGA-KO-THP-1 cell line.Transcription analysis revealed 742 upregulated and 1376 downregulated lncRNAs and 3276 upregulated and 213 downregulated mRNAs. After deep screening, 8 highly expressed mRNAs that were related to the NF-κB pathway were analyzed to determine coexpression patterns. LINC01002, FAM157C, CTD-2530H12.2, XLOC-064331 and XLOC-106677 were correlated with the 8 mRNAs. After measuring the expression of these molecules in 30 PNH patients by qRT‒PCR, lncRNA FAM157C was verified to be upregulated in the PNH clone, and its expression levels were positively correlated with the LDH levels and CD59- granulated and monocyte cell ratios. After knockdown of the FAM157C gene in the PIGA-KO-THP-1 cell line, we found that the cells were arrested in the G0/G1 phase and S phase, the apoptosis rate increased, and the cell proliferation decreased.LncRNA FAM157C was proven to promote PNH clone proliferation, and this is the first study to explore the role of lncRNAs in PNH., (© 2023. The Author(s).)- Published
- 2023
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6. Defected lipid rafts suppress cavin1-dependent IFN-α signaling endosome in paroxysmal nocturnal hemoglobinuria.
- Author
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Zeng L, Liu H, Liu Z, Li L, Wang H, Chen Y, Wu J, Wang G, Li L, and Fu R
- Subjects
- Humans, Endosomes metabolism, Hematopoietic Stem Cells, Membrane Microdomains metabolism, Membrane Proteins metabolism, RNA-Binding Proteins metabolism, Interferon-alpha metabolism, Hemoglobinuria, Paroxysmal genetics, Hemoglobinuria, Paroxysmal metabolism
- Abstract
Paroxysmal nocturnal haemoglobinuria (PNH) is a clonal disorder of haematopoietic stem cells caused by somatic PIGA mutations, resulting in a deficiency in glycosylphosphatidylinositol-anchored proteins (GPI-AP). Some researchers uncovered that PNH cells displayed a GPI-mediated defect in lipid-raft formation. However, Lipid rafts play a crucial role in signaling, the signaling underlying lipid rafts in PNH have not yet been addressed. In this study, we reported that, IFN-α was significantly increased in PNH plasma compared with normal controls. And PNH cells more resistant to the inhibitory colony[1]-forming activity of IFN-α. Here we have already established PIGA knock out K562 cell line by CRISPR/cas9, the most recognized in vitro model of PNH. PNH cells showed obviously defected endocytosis of IFNα/βRs in lipid rafts, causing suppressed STAT2 activation and the inflammatory response. We further investigated the possible mechanisms of interferon signaling endosomes mediate by cavin1. Our findings provide crucial insight into the process of reduced IFNα signal transduction in PNH cells mediated by lipid rafts and suggest that cavin1 are a potential target for suppression of IFN-α inflammatory signaling. These results might further explain the growth advantage of PNH cells in an unfavorable microenvironment., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)
- Published
- 2023
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7. SUZ12 participates in the proliferation of PNH clones by regulating histone H3K27me3 levels.
- Author
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Chen Y, Liu H, Zeng L, Li L, Lu D, Liu Z, and Fu R
- Subjects
- Cell Proliferation, Clone Cells metabolism, Humans, Hemoglobinuria, Paroxysmal genetics, Hemoglobinuria, Paroxysmal metabolism, Hemoglobinuria, Paroxysmal therapy, Histones, Neoplasm Proteins genetics, Transcription Factors genetics
- Abstract
Paroxysmal nocturnal hemoglobinuria (PNH) is a disease involving hematopoietic stem cell membrane defects caused by acquired phosphatidylinositol glycan anchor biosynthesis class A (PIGA) mutations. In this study, 97 target genes were selected as a target gene panel and screened in 23 PNH patients via the sequencing of specific DNA target regions. Through functional analysis, we identified that suppressor-of-Zeste 12 (SUZ12) may be involved in the proliferation of PNH clones. mRNA and protein expression levels of SUZ12 and the trimethylation level of histone H3 at lysine 27 (H3K27) in CD59
- peripheral blood leukocytes from PNH patients were higher than those in CD59+ cells from PNH patients and peripheral blood leukocytes from healthy controls. In addition, the relative expression of SUZ12 in PNH patients was positively correlated with Ret% and the proportion of PNH clones. When we knocked down SUZ12 expression in a PIGA knockdown THP-1 cell line (THP-1 KD cells), the trimethylation of histone H3K27(H3K27me3) and cell proliferation decreased, apoptosis increased, and cell cycle arrest occurred in G0/G1 phase. In conclusion, SUZ12 participates in the proliferation of PNH clones by regulating histone H3K27me3 levels. Our results may provide new therapeutic targets and possibilities for PNH patients., (©2022 Society for Leukocyte Biology.)- Published
- 2022
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8. Clinical observation of low-dose combination chemotherapy in refractory/recurrent paroxysmal nocturnal hemoglobinuria patients: A single-center retrospective analysis.
- Author
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Li L, Liu H, Wang H, Liu Z, Chen Y, Liu C, Zhao X, Li L, Wang H, Shao Z, and Fu R
- Subjects
- Adult, Aged, Female, Humans, Male, Middle Aged, Drug Resistance, Drug Therapy, Combination adverse effects, Retrospective Studies, Survival Rate, Cytarabine administration & dosage, Glucocorticoids administration & dosage, Hemoglobinuria, Paroxysmal drug therapy, Hemoglobinuria, Paroxysmal mortality, Methylprednisolone administration & dosage, Daunorubicin administration & dosage
- Abstract
Background: We performed a retrospective analysis to investigate the clinical characteristics and therapeutic strategies of 20 refractory/recurrent PNH patients, including the clinical efficacy of chemotherapy treatment, safety, and survival., Methods: The clinical data of 20 classic PNH patients who were refractory/recurrent or had glucocorticoid dependence in our hospital were analyzed, including clinical manifestations, laboratory examinations, treatment efficacy, and survival., Results: Seventeen patients had a marked improvement in anemia after chemotherapy, 14 patients acquired blood transfusion independence, and the Hb of 3 patients increased to normal levels. Although 6 patients still needed blood transfusion, the transfusion interval was significantly prolonged. The percentages of LDH, TBIL, and RET, which are indicators of hemolysis, were significantly lower than those before chemotherapy. The dosage of adrenal glucocorticoids was reduced by more than half compared with that before chemotherapy., Conclusions: Chemotherapy can reduce PNH clones, promote normal hematopoiesis, and control hemolytic attack. It is a promising and widely used therapeutic method., (© 2022 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.)
- Published
- 2022
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9. Relationship between immune status after ATG treatment and PNH clone evolution in patients with severe aplastic anemia.
- Author
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Wang H, Liu H, Wang T, Li L, Liu C, Li L, Chen T, Qi W, Ding K, and Fu R
- Subjects
- Adolescent, Adult, Aged, Anemia, Aplastic blood, CD8-Positive T-Lymphocytes, Child, Cyclosporine pharmacology, Female, Humans, Male, Middle Aged, Myelodysplastic Syndromes etiology, Retrospective Studies, Treatment Outcome, Young Adult, Anemia, Aplastic drug therapy, Anemia, Aplastic immunology, Antilymphocyte Serum therapeutic use, Hemoglobinuria, Paroxysmal immunology
- Abstract
Objectives: To investigate the relationship between immune status and paroxysmal nocturnal hemoglobinuria (PNH) clonal evolution of severe aplastic anemia (SAA) patients who received anti-human thymocyte globulin (ATG) treatment., Methods: The clinical data of 102 SAA patients who received ATG were collected and retrospectively analyzed. The remission rate, remission time, response rate, hematopoietic, and immune status were compared. Malignant clones were also observed., Results: The remission rate of the group with PNH clones appeared after treatment was significantly higher than the group without PNH clones. The response rate at 12 months of the groups with PNH clones was significantly higher than the group without PNH clones. The recovery of Hb and Ret % of patients with PNH clones was earlier than the patients without PNH clones. The reduction of percentage of CD8
+ HLA-DR+ /CD8+ and Th1/Th2 ratio of patients with PNH clones was both earlier than the patients without PNH clones. Six patients developed myelodysplastic syndromes (MDS)., Conclusion: In SAA patients with PNH clones, the cytotoxic T-cell function and Th1 cell number recovered more quickly and had better response to IST. A small number of SAA patients with or without PNH clones developed MDS malignant clones., (© 2020 The Authors. Journal of Clinical Laboratory Analysis Published by Wiley Periodicals LLC.)- Published
- 2021
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10. Analysis of clinical characteristics of 92 patients with paroxysmal nocturnal hemoglobinuria: A single institution experience in China.
- Author
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Fu R, Li L, Li L, Liu H, Zhang T, Ding S, Wang G, Song J, Wang H, Xing L, Guan J, and Shao Z
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, China, Female, Glucocorticoids adverse effects, Glucocorticoids therapeutic use, Hemoglobinuria, Paroxysmal physiopathology, Humans, Kidney Function Tests, Male, Middle Aged, Survival Analysis, Treatment Outcome, Young Adult, Hemoglobinuria, Paroxysmal pathology
- Abstract
Objectives: We performed a retrospective analysis to investigate the clinical characteristics and therapeutic strategies of Chinese paroxysmal nocturnal hemoglobinuria (PNH) patients, and assessed the efficacy and safety of glucocorticoid in PNH patients., Methods: The clinical data of 92 PNH cases in our hospital were analyzed, including clinical manifestation, laboratory examination, treatment efficacy, and survival., Results: The main clinical manifestations of these patients included hemoglobinuria, anemia, fatigue, dyspnea, headache, abdominal pain, and erectile dysfunction. Glucocorticoid is still the first-line treatment for PNH patients to control hemolytic attack, and the short-term remission rate (12 months) is 79.01% (64/81). Meanwhile, the overall survival (OS) of 10 years after diagnosis was estimated as 70.77% (46/65). Moreover, Cox proportional risk model for multivariate analysis showed that the increase in LDH multiple, thrombosis complications, and complicated with bone marrow failure were the independent adverse prognostic factors affecting the survival of PNH patients., Conclusion: Paroxysmal nocturnal hemoglobinuria patients in mainland China have various clinical features, while lower incidences of thrombosis and renal damage. Thrombosis and bone marrow failure are two complications with worse prognosis., (© 2019 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals, Inc.)
- Published
- 2020
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11. Gene mutations associated with thrombosis detected by whole-exome sequencing in paroxysmal nocturnal hemoglobinuria.
- Author
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Li L, Wang H, Liu H, Liu Z, Li L, Ding K, Wang G, Song J, and Fu R
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- Adult, Aged, Biomarkers, Computational Biology, DNA Mutational Analysis, Female, Flow Cytometry, Gene Expression, Genetic Association Studies, Humans, Male, Middle Aged, Thrombosis diagnosis, Young Adult, Genetic Predisposition to Disease, Hemoglobinuria, Paroxysmal complications, Hemoglobinuria, Paroxysmal genetics, Mutation, Thrombosis etiology, Exome Sequencing
- Abstract
Background: Thrombosis is a most common and lethal complication of paroxysmal nocturnal hemoglobinuria (PNH), which is a complex progression and its mechanism remains unclear. We tried to explore the possible genetic background of thrombosis in PNH patients and provide potential gene mutations associated with thrombosis in PNH patients., Methods: The CD59
- cells of 7 PNH and 6 PNH- aplastic anemia (AA) patients were sorted by flow cytometry and sequenced by whole-exome sequencing (WES). The sequencing results and target mutation genes were analyzed and screened, respectively, and Kyoto Encyclopedia of Genes and Genomes (KEGG) signal pathway enrichment analysis was carried out. Finally, the expression of target genes was detected in 22 PNH (including seven cases with thrombus) and 20 normal controls, and the correlation between the expression of mRNA and the clinical thrombus-related indexes was analyzed., Results: The mutation genes screened from 4 PNH with thrombus were BMPR2, F8, ITGA2B, THBD, and THBS1. The pathways enriched by these genes included Notch, Wnt, and arachidonic acid metabolism signaling pathways, which may be related to the pathogenesis of thrombosis in PNH. The BMPR2, THBD, and THBS1 gene expression was significantly different between PNH with and without thrombus group, and the THBS1 gene expression was positively correlated with D-Dimer and su-PAR levels., Conclusions: Genetic defects have a non-negligible effect on the incidence of thrombosis, and therefore, gene mutations maybe a genetic risk factor in PNH, which increase the incidence of thrombosis., (© 2019 John Wiley & Sons Ltd.)- Published
- 2019
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12. [Study on C5b-9 deposited on the membrane of platelets and its dysfunction in patients with paroxysmal nocturnal hemoglobinuria].
- Author
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Meng Y, Fu R, Liu H, Wang Y, Li L, Liu C, Zhang T, Ding S, Li L, Ruan E, Qu W, Wang H, Wang X, Wang G, Liu H, Wu Y, Song J, Xing L, Guan J, and Shao Z
- Subjects
- Anemia, Aplastic, Clone Cells, Complement Membrane Attack Complex, Flow Cytometry, Humans, P-Selectin, Thrombosis, Blood Platelets, Hemoglobinuria, Paroxysmal
- Abstract
Objective: To explore the expression levels of terminal complement complex (C5b-9) and CD62p on platelets and the soluble C5b-9 (sC5b-9) level in serum in patients with PNH or PNH-aplastic anemia (AA)., Methods: Serum levels of sC5b-9, complement C3 and C4 were detected by using ELISA in 25 patients with PNH/PNH-AA. The quantities of C5b-9 and CD62p on the membrane of platelets were detected by flow cytometry., Results: ①In PNH/PNH-AA group, the serum sC5b-9 level [390.27(265.73-676.87) μg/L] was lower than that in control group [540.39(344.20-1 576.78) μg/L] (P<0.01). ②The platelet PNH clone (CD59⁻CD61⁺/CD61⁺) size [50.58(23.29-81.60)%] was bigger in the PNH/PNH-AA group than that [23.57(15.58-29.02)%] in control group (P<0.01). The percentages of C5b-9 deposition (C5b-9⁺CD61⁺/CD61⁺) were higher on the PNH clone platelets (CD59⁻CD61⁺) in the PNH/PNH-AA group [(17.53 ± 6.27)%] than those on the normal platelets (CD59⁺CD61⁺) in PNH patients 11.33±5.03)%] and control [(10.88±3.58)%] group (P<0.01). ③ The expression of CD62p (CD62p⁺CD61⁺/CD61⁺) on PNH clone platelets in PNH patients [(61.98 ± 11.71)%] was higher than that on the normal platelets in PNH patients [(43.76±11.30)%] and control group [(38.23±18.07)%] (P<0.01). In addition, the expression of CD62p on normal platelets was higher in PNH patients than control (P<0.05). ④The deposition of C5b-9 positively correlated with the expression of CD62p on the platelets (r=0.559, P=0.002)., Conclusion: Deficiency of CD59 antigen on platelets in PNH patients may lead to the deposition of C5b-9 on its membrane and its dysfunction, which may contribute to thrombosis events in PNH.
- Published
- 2015
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13. [Abnormal WT1 gene expression in paroxysmal nocturnal hemoglobinuria].
- Author
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Zhang Y, Fu R, Wang Y, Li L, Liu H, Liu C, Zhang T, Ding S, Li L, Ruan E, Qu W, Wang H, Wang X, Wang G, Wu Y, Song J, Liu H, Xing L, Guan J, and Shao Z
- Subjects
- Adolescent, Adult, Aged, Apoptosis, Bone Marrow Cells metabolism, Cell Cycle, Female, Hemoglobinuria, Paroxysmal metabolism, Humans, Leukocytes, Mononuclear metabolism, Male, Middle Aged, RNA Interference, RNA, Messenger genetics, WT1 Proteins genetics, Young Adult, Hemoglobinuria, Paroxysmal pathology, WT1 Proteins metabolism
- Abstract
Objective: To explore the pathogenesis of abnormal WT1 expression in paroxysmal nocturnal hemoglobinuria (PNH)., Methods: The expression of WT1 mRNA in CD59⁻ and CD59⁺ bone marrow mononuclear cells (BMMNC) were measured by semi-quantitative reverse transcription PCR. After WT1 gene silence by RNA interference (RNAi) technology, biological characteristics of BMMNC were investigated by flow cytometry., Results: The relative expression of WT1 mRNA in PNH CD59⁻ BMMNC (1.06 ± 0.12) was significantly higher than that in PNH CD59⁺ BMMNC (0.90 ± 0.12) and normal BMMNC (0.86 ± 0.05, P<0.05), but there was no significant difference between PNH CD59⁺ BMMNC and normal BMMNC (P>0.05). WT1 mRNA expression in PNH was positively correlated with the proportion of CD59⁻ cells (r²=0.490, P=0.016), but had no relationship with the proportion of CD59⁺ cells. After WT1 gene silence by siRNA in PNH CD59⁻ BMMNC, WT1 mRNA expression was decreased. The proportions of G0/G1 phase in PNH CD59⁻ cell blank control group and siRNA-scr transfected group were (92.73 ± 3.71)% and (93.06 ± 4.14)%, and the proportions of S phase were (6.99 ± 3.61)% and (6.73 ± 4.08)%, respectively. The proportions of G0/G1 and S phase in siRNA-WT1 transfected group was (94.46 ± 3.71)% and (5.40 ± 3.55)%, respectively. There were significant differences in the proportions of G0/G1 phase and S phase among the controls, siRNA-WT1 transfected group and siRNA-scr transfected group (P<0.05). The rate of apoptosis in siRNA-WT1 transfected group [(35.91 ± 22.36)%] was significantly higher than those in controls [(26.12 ± 17.10)%] and siRNA-scr transfected group [(27.39 ± 18.99)%] (P<0.05)., Conclusion: siRNA-WT1 could effectively suppress the WT1 gene expression of CD59⁻ clone in PNH patients, inhibit its proliferation, and promote its apoptosis. WT1 gene expression might contribute to PNH clone proliferation.
- Published
- 2014
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