1. Terminal stages of complement hemolysis: technical comment.
- Author
-
Wirtz GH and Westfall SS
- Subjects
- Animals, Complement C8 metabolism, Complement C9 metabolism, Edetic Acid pharmacology, Erythrocytes metabolism, Guinea Pigs, Temperature, Time Factors, Complement System Proteins metabolism, Hemolysis, Immunologic Techniques
- Abstract
In studying terminal lytic events of sensitized erythrocytes which have reacted with all 9 components of complement, we agree in general with the conclusions of Boyle and Borsos [1] that the cellular intermediate passes through the stages E*bound, E*inserted, and E*doomed. However, we differ with these workers on the influence of 3 variables on the terminal reactions. These variables are: time of hemolysis; temperature of E* preparation; and the use of EDTA to manipulate E*.
- Published
- 1981
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