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Irregular hemorrhagic traumas always threaten the health of patients due to uncontrollable bleeding and wound infections. The traditional hemostatic materials show dissatisfactory hemostatic efficiency and antibacterial activity in solving these potential bleeding dangers. Herein, we proposed a kind of composites based on flexible wood membrane (FWM) loaded with chitosan/alginate derivative for accelerating rapid hemostasis and preventing infection. FWM was removed part of hemicellulose and lignin by using NaOH/Na 2 SO 3 mixture to obtain excellent flexibility while retaining the original porous structure, followed by loading silver nanoparticles on the FWM surface to prepare AgNPs-FWM as an antibacterial bio-carrier. Then, AgNPs-FWM was coated with polyoxyethylene stearate-modified chitosan and multi-aldehyde sodium alginate to fabricate the composites of chitosan/alginate/AgNPs-FWM (CSA/AgNPs-FWM) using in-situ Schiff base reaction. Furthermore, in vitro and in vivo experiments showed that the CSA/AgNPs-FWM composites exhibited lower BCI value (2.6 ± 1.3 %), more rapid hemostasis (26 s) and lower blood loss (67.8 mg) than that of the traditional materials. The possible mechanism for the hemostasis process was not only the high blood absorption capacity, but also the synergistic interaction between hydrophobic alkane chains, amino groups, aldehydes, hydroxyl groups and blood cells. Moreover, CSA/AgNPs-FWM showed exceptional superiorities in mechanical properties and antibacterial activity, which endowed composites high potential in hemostasis application for irregular external wound. • FWM with native porously microstructure and flexibility was designed as a green and safe biocarrier. • CSA/AgNPs-FWM could aggregate and stimulate blood cells/platelets, promoting blood coagulation. • CSA/AgNPs-FWM could achieve shape adaptation to the surrounding tissues and the wound cavities to protect injured tissue. • CSA/AgNPs-FWM exhibits rapid hemostasis and long-term antibacterial properties. [ABSTRACT FROM AUTHOR]

Multifunctional wound dressings are promising medical materials for various applications. Among them, dressings with antimicrobial activity, high biosafety, and real-time monitoring have attracted considerable research interest. Herein, a biodegradable hemostatic sponge comprising a chitosan skeleton and polyelectrolyte–surfactant complex (CS–PEC) was developed as a versatile wound dressing for wound pH monitoring and inhibition of bacterial infection. CS–PEC sponge with high porosity exhibited satisfactory fluid absorption capacity and biocompatibility, along with antibacterial properties against E. coli and S. aureus. In vivo experiments in rat liver trauma model revealed that wounds treated with the CS–PEC sponge recorded less blood loss (97.1 mg) and shorter hemostasis time (27.2 s) than those treated with commercial gelatin sponge (309.1 mg and 163.5 s, respectively). Furthermore, PECs based on unconventional luminescent molecules (L -C 16 -Hyp) were used as pH fluorescent indicators, which endowed the sponge with fluorescence-responsive behavior to wound pH changes in the range of 5.0–8.5. Visual images can be captured using a smartphone and converted to RGB color mode values for on-site assessment of wound status. This study sheds light on the design and application of unconventional luminescent materials in wound dressing and provides a smart and effective solution for wound management. [ABSTRACT FROM AUTHOR]

Herein, a novel bioinspired radial porous zinc-based metal-organic framework (Zn-MOF) doped sodium alginate/chitosan derivatives/pullulan-based SA/PSCS/Pul/Zn-MOF (SPCP/Zn) composites sponge with excellent antioxidant and antibacterial properties was fabricated by the ice-templating method. Boric acid (BA) and Ca2+, which were respectively used as hydrogen- and ionic- bonding cross-linkers, provided strong mechanical properties for sponge matrix composed of SA, PSCS, and Pul. The obtained SPCP/Zn sponge exhibited uniform porous morphology, proper hydrophilicity, and admirable biocompatibility. In addition, the SPCP/Zn sponge achieved a sustained release of Zn2+ and gallic acid, which displayed powerful antibacterial and antioxidant activities. Importantly, the SPCP/Zn sponge exhibited shorter rapid hemostasis (20.4 ± 2.9 s) and lower blood loss (19.8 ± 4.3 mg). The SPCP/Zn sponge also showed faster wound closure ratio for the rat full-thickness skin defect model. It was revealed that SPCP/Zn sponge could significantly accelerate and enhance wound healing through downregulating inflammatory cytokines (TNF-α, IL-6) and increasing the expression of growth factors (VEGF). Due to its excellent properties, the SPCP/Zn sponge may have promising potential in wound healing applications. [ABSTRACT FROM AUTHOR]

Effective management of excessive bleeding requires liquid hemostatic agents, especially in scenarios involving uncompressible and postoperative hemorrhage. This study introduces the microbial exopolysaccharide floccuronic acid (FA) as a liquid hemostatic agent, characterized by a high weight average molecular weight of 2.38 × 108 Da. The investigation focuses on the flocculation effect, hemostatic efficiency in both in vitro and in vivo settings, elucidating its hemostatic mechanism, and assessing its safety profile. Results reveal that FA solution significantly accelerates the coagulation process, leading to the formation of compact clots while specifically interfering with fibrin. Notably, FA demonstrates excellent hemostatic effects in animal liver models and a rat arterial rebleeding model. The biocompatible and biodegradable characteristics further underscore FA's potential as a valuable liquid hemostatic material, particularly suited for non-compressible and re-bleeding scenarios. [ABSTRACT FROM AUTHOR]