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[Display omitted] • Stability, degradation and in vitro simulation characteristics of emulsions. • Oleogel-in-water emulsion with 3 % hemp seed protein had better stability. • The half-life of cannabinol in oleogel-in-water emulsion was 131.82 days. • Bioaccessibility of cannabinol in oleogel-in-water emulsion was 58.0 %. The present study focused on investigating the stability and in vitro simulation characteristics of oil-in-water (O/W) and oleogel-in-water (Og/W) emulsions. Compared with O/W emulsion, the Og/W emulsion exhibited superior stability, with a more evenly spread droplet distribution, and the Og/W emulsion containing 3 % hemp seed protein (HSP) showed better stability against environmental factors, including heat treatment, ionic strength, and changes in pH. Additionally, the stability of Δ9-tetrahydrocannabinol (Δ9-THC) and cannabinol (CBN) and the in vitro digestion of hemp seed oil (HSO) were evaluated. The half-life of CBN in the Og/W emulsion was found to be 131.82 days, with a degradation rate of 0.00527. The in vitro simulation results indicated that the Og/W emulsion effectively delayed the intestinal digestion of HSO, and the bioaccessibility of Δ9-THC and CBN reached 56.0 % and 58.0 %, respectively. The study findings demonstrated that the Og/W emulsion constructed with oleogel and HSP, exhibited excellent stability. [ABSTRACT FROM AUTHOR]

Hemp seed protein (HSP) stabilized emulsion was subjected to dynamic high-pressure microfluidization (DHPM). Gum Arabic (GA) was added to the emulsion system to prepare HSP-GA bilayer emulsion, which improved the stability of hemp seed oil (HSO). Under the DHPM treatment at 120 MPa, the particle size of HSP-GA bilayer emulsion was 260.02 nm and the zeta potential was − 25.9 mV. The interfacial adsorbed protein of the DHPM-treated emulsion increased to 37.35%. And the viscosity, the storage modulus (G'), and loss modulus (G") were increased. The HSP-GA emulsion under 120 MPa was selected to develop an oxidation kinetic model to obtain equation c = e 10.56086 e − 3659.66658 T * t + 0.72 , which was used to predict the POV variation of HSO within the bilayer emulsion system. The CBD retention rate was 93.54% during storage for 20 days. It can be concluded that the effective encapsulation of DHPM treatment and bilayer emulsion system is beneficial to broaden the application of hemp seeds in processing industry. [Display omitted] • The bilayer emulsion stabilized by hemp seed protein and Gum Arabic was established. • The primary emulsions were subjected to dynamic high-pressure microfluidization. • Bilayer emulsions provide better rheological properties and oxidative stability. • Oxidation kinetic model was established, was c = e 10.56086 e − 3659.66658 T * t + 0.72. [ABSTRACT FROM AUTHOR]