1. Clearance of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Undergoing Surgical Resection or Liver Transplantation
- Author
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Manuel Rodríguez-Perálvarez, Javier Zamora, Gema Garcia-Jurado, Marina E. Sánchez-Frías, María Lola Espejo-Cruz, Manuel de la Mata, Ruben Ciria, Clara I. Linares, Víctor Amado, Sandra González-Rubio, Gustavo Ferrín, Rafael Alejandre, José Luis Montero, [Amado,V, Zamora,J, Alejandre,R, Montero,JL, Rodríguez-Perálvarez,M, De la Mata,M] Department of Hepatology and Liver Transplantation, Reina Sofía University Hospital, Córdoba, Spain. [Amado,V, González-Rubio,S, Espejo-Cruz,ML, Linares,C, García-Jurado,G, Ciria,R, Ferrín,G, De la Mata,M] Maimónides Institute of Biomedical Research (IMIBIC), University of Córdoba, Córdoba, Spain. [González-Rubio,S, De la Mata,M] Centro de Investigación Biomédica en Red de Enfermedades hepáticas y digestivas (CIBERehd), Madrid, Spain. [Sánchez-Frías,M] Pathology Department, Reina Sofía University Hospital, Córdoba, Spain. [Ciria,R] Department of Hepatobiliary Surgery and Liver Transplantation, Reina Sofía University Hospital, Córdoba, Spain., and This research was funded by the Instituto de Salud Carlos III (grant number PI14/01469 and PI18/01736) and co-funded by the Fondo Europeo de Desarrollo Regional (FEDER).
- Subjects
Cancer Research ,Diseases::Virus Diseases::Hepatitis, Viral, Human::Hepatitis C::Hepatitis C, Chronic [Medical Subject Headings] ,medicine.medical_treatment ,Liver transplantation ,Gastroenterology ,Neoplasias hepáticas ,Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] ,Liver disease ,0302 clinical medicine ,Circulating tumor cell ,RC254-282 ,Recurrencia local de neoplasia ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Surgical Procedures, Operative::Digestive System Surgical Procedures::Liver Transplantation [Medical Subject Headings] ,liver transplantation ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,hepatocellular carcinoma ,Oncology ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,030211 gastroenterology & hepatology ,Liver cancer ,Carcinoma hepatocelular ,Chemicals and Drugs::Biological Factors::Biological Markers::Biomarkers, Pharmacological [Medical Subject Headings] ,Diseases::Neoplasms::Neoplastic Processes::Neoplasm Metastasis::Neoplastic Cells, Circulating [Medical Subject Headings] ,Tumor recurrence ,medicine.medical_specialty ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cohort Studies::Longitudinal Studies::Prospective Studies [Medical Subject Headings] ,Check Tags::Male [Medical Subject Headings] ,Diseases::Digestive System Diseases::Liver Diseases::Liver Cirrhosis [Medical Subject Headings] ,circulating tumor cell ,Article ,Health Care::Health Care Facilities, Manpower, and Services::Health Services::Tissue and Organ Procurement [Medical Subject Headings] ,liver cancer ,03 medical and health sciences ,Diseases::Digestive System Diseases::Liver Diseases::Liver Neoplasms::Carcinoma, Hepatocellular [Medical Subject Headings] ,Internal medicine ,medicine ,Liquid biopsy ,Diseases::Digestive System Diseases::Liver Diseases::Liver Neoplasms [Medical Subject Headings] ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Pilot Projects [Medical Subject Headings] ,liquid biopsy ,Trasplante de hígado ,business.industry ,Surrogate endpoint ,medicine.disease ,Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Neoplastic Processes::Neoplasm Recurrence, Local [Medical Subject Headings] ,Biopsia líquida ,Etiology ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Prognosis [Medical Subject Headings] ,business ,Células neoplásicas circulantes - Abstract
Background: In patients with hepatocellular carcinoma (HCC), a complete clearance of circulating tumor cells (CTCs) early after liver transplantation (LT) or surgical resection (LR) could prevent tumor recurrence. Methods: prospective pilot study including patients with HCC who underwent LR or LT from September 2017 to May 2020. Enumeration of CTCs was performed in peripheral blood samples (7 mL) using the Isoflux® system (Fluxion Biosciences) immediately before surgery, at post-operative day 5 and at day 30. A clinically relevant number of CTCs was defined as >, 30 CTCs/sample. Results: 41 HCC patients were included (mean age 58.7 ± 6.3, 82.9% male). LR was performed in 10 patients (24.4%) and 31 patients (75.6%) underwent LT. The main etiology of liver disease was chronic hepatitis C (31.7%). Patients undergoing LR and LT were similar in terms of preoperative CTC count (p = 0.99), but clearance of CTCs within the first month was more pronounced in the LT group. Clusters of CTCs at baseline were associated with incomplete clearance of CTCs at day 30 (54.2% vs. 11.8%, p = 0.005), which in turn impacted negatively on survival (p = 0.038). Conclusion: Incomplete clearance of CTCs after surgery could be a surrogate marker of HCC aggressiveness.
- Published
- 2021