11 results on '"Sue, Carolyn M."'
Search Results
2. Outcome Measures and Biomarkers for Clinical Trials in Hereditary Spastic Paraplegia: A Scoping Review.
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Siow, Sue-Faye, Yeow, Dennis, Rudaks, Laura I., Jia, Fangzhi, Wali, Gautam, Sue, Carolyn M., and Kumar, Kishore R.
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FAMILIAL spastic paraplegia ,DIFFUSION tensor imaging ,CLINICAL trials ,PATIENT reported outcome measures ,MAGNETIC resonance imaging - Abstract
Hereditary spastic paraplegia (HSP) is characterized by progressive lower limb spasticity. There is no disease-modifying treatment currently available. Therefore, standardized, validated outcome measures to facilitate clinical trials are urgently needed. We performed a scoping review of outcome measures and biomarkers for HSP to provide recommendations for future studies and identify areas for further research. We searched Embase, Medline, Scopus, Web of Science, and the Central Cochrane database. Seventy studies met the inclusion criteria, and eighty-three outcome measures were identified. The Spastic Paraplegia Rating Scale (SPRS) was the most widely used (27 studies), followed by the modified Ashworth Scale (18 studies) and magnetic resonance imaging (17 studies). Patient-reported outcome measures (PROMs) were infrequently used to assess treatment outcomes (28% of interventional studies). Diffusion tensor imaging, gait analysis and neurofilament light chain levels were the most promising biomarkers in terms of being able to differentiate patients from controls and correlate with clinical disease severity. Overall, we found variability and inconsistencies in use of outcome measures with a paucity of longitudinal data. We highlight the need for (1) a standardized set of core outcome measures, (2) validation of existing biomarkers, and (3) inclusion of PROMs in HSP clinical trials. [ABSTRACT FROM AUTHOR]
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- 2023
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3. Defining the genetic basis of early onset hereditary spastic paraplegia using whole genome sequencing
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Kumar, Kishore R, Wali, G.M., Kamate, Mahesh, Wali, Gautam, Minoche, André E, Puttick, Clare, Pinese, Mark, Gayevskiy, Velimir, Dinger, Marcel E, Roscioli, Tony, Sue, Carolyn M., and Cowley, Mark J
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- 2016
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4. Reduced acetylated α-tubulin in SPAST hereditary spastic paraplegia patient PBMCs.
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Wali, Gautam, Siow, Sue-Faye, Liyanage, Erandhi, Kumar, Kishore R., Mackay-Sim, Alan, and Sue, Carolyn M.
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FAMILIAL spastic paraplegia ,PEOPLE with paraplegia ,MONONUCLEAR leukocytes ,INDUCED pluripotent stem cells ,DRUG discovery - Abstract
HSP-SPAST is the most common form of hereditary spastic paraplegia (HSP), a neurodegenerative disease causing lower limb spasticity. Previous studies using HSP-SPAST patient-derived induced pluripotent stem cell cortical neurons have shown that patient neurons have reduced levels of acetylated a-tubulin, a form of stabilized microtubules, leading to a chain of downstream effects eventuating in increased vulnerability to axonal degeneration. Noscapine treatment rescued these downstream effects by restoring the levels of acetylated a-tubulin in patient neurons. Here we show that HSP-SPAST patient non-neuronal cells, peripheral blood mononuclear cells (PBMCs), also have the disease-associated effect of reduced levels of acetylated a-tubulin. Evaluation of multiple PBMC subtypes showed that patient T cell lymphocytes had reduced levels of acetylated a-tubulin. T cells make up to 80% of all PBMCs and likely contributed to the effect of reduced acetylated a-tubulin levels seen in overall PBMCs. We further showed that mouse administered orally with increasing concentrations of noscapine exhibited a dose-dependent increase of noscapine levels and acetylated a-tubulin in the brain. A similar effect of noscapine treatment is anticipated in HSP-SPAST patients. To measure acetylated a-tubulin levels, we used a homogeneous time resolved fluorescence technologybased assay. This assay was sensitive to noscapine-induced changes in acetylated a-tubulin levels in multiple sample types. The assay is high throughput and uses nano-molar protein concentrations, making it an ideal assay for evaluation of noscapine-induced changes in acetylated a-tubulin levels. This study shows that HSP-SPAST patient PBMCs exhibit disease-associated effects. This finding can help expedite the drug discovery and testing process. [ABSTRACT FROM AUTHOR]
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- 2023
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5. Targeted next generation sequencing in SPAST-negative hereditary spastic paraplegia
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Kumar, Kishore R., Blair, Nicholas F., Vandebona, Himesha, Liang, Christina, Ng, Karl, Sharpe, David M., Grünewald, Anne, Gölnitz, Uta, Saviouk, Viatcheslav, Rolfs, Arndt, Klein, Christine, and Sue, Carolyn M.
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- 2013
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6. Mitochondrial Function in Hereditary Spastic Paraplegia: Deficits in SPG7 but Not SPAST Patient-Derived Stem Cells.
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Wali, Gautam, Kumar, Kishore Raj, Liyanage, Erandhi, Davis, Ryan L., Mackay-Sim, Alan, and Sue, Carolyn M.
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MITOCHONDRIAL pathology ,FAMILIAL spastic paraplegia ,STEM cells ,EPICATECHIN ,OXIDATIVE phosphorylation ,MEMBRANE potential ,MITOCHONDRIAL membranes ,CELL proliferation - Abstract
Mutations in SPG7 and SPAST are common causes of hereditary spastic paraplegia (HSP). While some SPG7 mutations cause paraplegin deficiency, other SPG7 mutations cause increased paraplegin expression. Mitochondrial function has been studied in models that are paraplegin-deficient (human, mouse, and Drosophila models with large exonic deletions, null mutations, or knockout models) but not in models of mutations that express paraplegin. Here, we evaluated mitochondrial function in olfactory neurosphere-derived cells, derived from patients with a variety of SPG7 mutations that express paraplegin and compared them to cells derived from healthy controls and HSP patients with SPAST mutations, as a disease control. We quantified paraplegin expression and an extensive range of mitochondrial morphology measures (fragmentation, interconnectivity, and mass), mitochondrial function measures (membrane potential, oxidative phosphorylation, and oxidative stress), and cell proliferation. Compared to control cells, SPG7 patient cells had increased paraplegin expression, fragmented mitochondria with low interconnectivity, reduced mitochondrial mass, decreased mitochondrial membrane potential, reduced oxidative phosphorylation, reduced ATP content, increased mitochondrial oxidative stress, and reduced cellular proliferation. Mitochondrial dysfunction was specific to SPG7 patient cells and not present in SPAST patient cells, which displayed mitochondrial functions similar to control cells. The mitochondrial dysfunction observed here in SPG7 patient cells that express paraplegin was similar to the dysfunction reported in cell models without paraplegin expression. The p.A510V mutation was common to all patients and was the likely species associated with increased expression, albeit seemingly non-functional. The lack of a mitochondrial phenotype in SPAST patient cells indicates genotype-specific mechanisms of disease in these HSP patients. [ABSTRACT FROM AUTHOR]
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- 2020
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7. Oxidative Stress-Induced Axon Fragmentation Is a Consequence of Reduced Axonal Transport in Hereditary Spastic Paraplegia SPAST Patient Neurons.
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Wali, Gautam, Liyanage, Erandhi, Blair, Nicholas F., Sutharsan, Ratneswary, Park, Jin-Sung, Mackay-Sim, Alan, and Sue, Carolyn M.
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FAMILIAL spastic paraplegia ,AXONAL transport ,PEOPLE with paraplegia ,PARAPLEGIA ,AXONS ,NEURONS ,ORGANELLES - Abstract
Hereditary spastic paraplegia (HSP) is a group of inherited disorders characterized by progressive spasticity and paralysis of the lower limbs. Autosomal dominant mutations in SPAST gene account for ∼40% of adult-onset patients. We have previously shown that SPAST patient cells have reduced organelle transport and are therefore more sensitive to oxidative stress. To test whether these effects are present in neuronal cells, we first generated 11 induced pluripotent stem (iPS) cell lines from fibroblasts of three healthy controls and three HSP patients with different SPAST mutations. These cells were differentiated into FOXG1-positive forebrain neurons and then evaluated for multiple aspects of axonal transport and fragmentation. Patient neurons exhibited reduced levels of SPAST encoded spastin, as well as a range of axonal deficits, including reduced levels of stabilized microtubules, lower peroxisome transport speed as a consequence of reduced microtubule-dependent transport, reduced number of peroxisomes, and higher density of axon swellings. Patient axons fragmented significantly more than controls following hydrogen peroxide exposure, suggesting for the first time that the SPAST patient axons are more sensitive than controls to the deleterious effects of oxidative stress. Treatment of patient neurons with tubulin-binding drugs epothilone D and noscapine rescued axon peroxisome transport and protected them against axon fragmentation induced by oxidative stress, showing that SPAST patient axons are vulnerable to oxidative stress-induced degeneration as a consequence of reduced axonal transport. [ABSTRACT FROM AUTHOR]
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- 2020
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8. Motor Evoked Potentials in Hereditary Spastic Paraplegia—A Systematic Review.
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Siow, Sue-Faye, Cameron Smail, Ruaridh, Ng, Karl, Kumar, Kishore R., and Sue, Carolyn M.
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META-analysis ,CLINICAL drug trials ,EFFERENT pathways ,DRUG efficacy ,LEG - Abstract
Background: Hereditary Spastic Paraplegia (HSP) is a slowly progressive neurodegenerative disorder with no disease modifying treatment. Potential therapeutic approaches are emerging and large-scale clinical drug trials for patients with HSP are imminent. A sensitive biomarker to measure the drug efficacy in these trials is required. Motor evoked potentials (MEPs) are a potential biomarker for HSP as they assess the central motor pathways and can be standardized with set protocols and guidelines. Objectives: We performed a systematic review to investigate the utility of MEPs as a diagnostic and disease severity biomarker for HSP. Search Methods: Systematic searches of PubMed, Embase, Medline, and Scopus were performed. Selection Criteria: Studies reporting on central motor conduction time measured with MEPs in adult and pediatric patients with HSP were included. We excluded studies in non-HSP patient cohorts, not in English, not original research, and unpublished journal articles. Data Collection and analysis: Search results were de-duplicated and screened according to the inclusion and exclusion criteria. The included papers were reviewed independently by two reviewers and data was collected on patient cohorts, test methods, results, and study quality. Results were analyzed using descriptive methods. Results: Of the 882 search results, 32 studies were included in the review. The most common finding was absent or prolonged lower limb (LL) central motor conduction time (CMCT) in patients with HSP (78% of patients studied). Quality assessment revealed variability in study methodology and reporting of results. Variations included patient cohorts of various genotypes as well as variations in equipment and techniques used. Aside from CMCT, none of the MEP parameter measures correlated with disease severity and many did not show significant difference between HSP patients and controls. Conclusion: Systematic review of MEP studies in HSP patient cohorts demonstrated mixed findings. Lower limb CMCT was the most promising parameter in terms of differentiating HSP patients from controls, with one study demonstrating a weak correlation with clinical disease severity. It is possible that the lack of consistency in study methodologies and small patient cohorts have contributed to the variable findings. A longitudinal study of MEPs in a large cohort of HSP patients with the same genotype will help clarify the utility of MEPs as a biomarker for disease severity and use in clinical trials. [ABSTRACT FROM AUTHOR]
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- 2019
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9. An Update on the Hereditary Spastic Paraplegias: New Genes and New Disease Models.
- Author
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Kumar, Kishore R., Blair, Nicholas F., and Sue, Carolyn M.
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LEG diseases ,PARAPLEGIA ,GENETIC disorders ,PARAPARESIS ,PROTEIN-protein interactions ,MOLECULAR diagnosis ,PLURIPOTENT stem cells - Abstract
Aims The hereditary spastic paraplegias ( HSPs) are a heterogeneous group of disorders characterized by spasticity in the lower limbs. We provide an overview of HSP with an emphasis on recent developments. Methods A PubMed search using the term 'hereditary spastic paraplegia' and 'hereditary spastic paraparesis' was conducted for a period from January 2012 to January 2015. We discuss and critique the major studies in the field over this 36-month period. Results A total of 346 publications were identified, of which 47 were selected for review. We provide an update of the common forms of HSP and include patient videos. We also discuss how next-generation sequencing (NGS) has led to the accelerated discovery of new HSP genes, including B4 GALNT1, DDHD1, C19orf12, GBA2, TECPR2, DDHD2, C12orf65, REEP2, and IBA57. Moreover, a single study alone identified 18 previously unknown putative HSP genes and created a model for the protein interactions of HSP, called the ' HSPome.' Many of the newly reported genes cause rare, complicated, autosomal recessive forms of HSP. NGS also has important clinical applications by facilitating the molecular diagnosis of HSP. Furthermore, common genetic forms of HSP have been studied using new disease models, such as neurons derived from induced pluripotent stem cells. These models have been used to elucidate important disease mechanisms and have served as platforms to screen for candidate drug compounds. Conclusion The field of HSP research has been progressing at a rapid pace. The challenge remains in translating these advances into new targeted disease therapies. [ABSTRACT FROM AUTHOR]
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- 2015
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10. Peripheral neuropathy in hereditary spastic paraplegia due to spastin (SPG4) mutation – A neurophysiological study using excitability techniques
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Kumar, Kishore R., Sue, Carolyn M., Burke, David, and Ng, Karl
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PERIPHERAL neuropathy , *PARAPLEGIA , *NEUROPATHY , *SPASTICITY , *NEUROPHYSIOLOGY , *GENETIC mutation - Abstract
Abstract: Objective: To identify peripheral nerve abnormalities in hereditary spastic paraplegia (HSP) due to mutations in the spastin gene (spastic paraplegia 4, SPG4) using standard nerve conduction (NCS) and novel tests of axonal excitability. Methods: Eleven patients with known mutations in spastin were assessed with NCS for the upper and lower limbs, and axonal excitability testing on the median nerve. Results: Standard nerve conduction studies revealed a sensorimotor neuropathy in two patients. Excitability studies on median motor axons showed an isolated abnormality (increased strength-duration time constant), but those on sensory axons were normal in nine patients with normal routine nerve conduction studies. Conclusions: Peripheral neuropathy occurs in HSP patients with SPG4 mutations, but axonal excitability studies provide limited additional evidence for subclinical peripheral nerve dysfunction, and add little further to standard nerve conduction studies. Significance: The features of HSP due to SPG4 mutations include sensorimotor polyneuropathy. The value of excitability studies is limited in individual patients. [Copyright &y& Elsevier]
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- 2012
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11. Outcome measures for hereditary spastic paraplegia clinical trials: Learnings from an Australian HSP center.
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Siow, Sue Faye, Waters, Amy, Coward, Sharon, Wali, Gautam, Ng, Karl, Sue, Carolyn M., and Kumar, Kishore R.
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FAMILIAL spastic paraplegia , *CLINICAL trials , *QUALITY of life measurement - Abstract
• SARA is recommended to supplement SPRS given ataxia is a frequent complication of genotypes such as SPG7. • A disease-specific patient reported outcome measure is for sensitive measurement of quality of life. [ABSTRACT FROM AUTHOR]
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- 2024
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