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The present study emphasizes the production and optimization of prodigiosin (PG) pigment from Serratia marcescens strain CSK, which was isolated from Shevaroy Hills, Salem district, Tamil Nadu, India. The response surface methodology analysis was applied for the optimization process of PG production. The maximum production of PG (2950 mg/L) was obtained at pH 7.0 with the addition of tryptophan (4.0 g/L) and sucrose (3.0 g/L) with 60 h of incubation. Further, the PG was characterized using high‐performance liquid chromatography, Fourier‐transform infrared spectroscopy, and gas chromatography‐mass spectrometry. The purified PG exhibited strong antioxidant and antibacterial activities. Also, PG's cytotoxic effects against human breast cancer (MCF‐7) cells were observed through acridine orange‐ethidium bromide (AO‐EB) and Hoechst staining. Molecular dockingstudies revealed that PG could bind positively to the caspase‐3 (breast cancer protein 1RE1) binding site with a binding energy score of 17.37 kcal/mol. Overall, the novel PG was found to be an anticancer drug for potential applications in the pharmaceutical industry. [ABSTRACT FROM AUTHOR]

The present study explored the immobilization of laccase onto iron magnetic nanoparticles (MNPs) to enhance its enzymatic properties and applications. The immobilization process was optimized using Box–Behnken design (BBD). BBD showed significance towards the quadratic model with experimental data. Maximum laccase activity recovery (99%) of the predicted model was observed at 0.75 mg/mL of laccase concentration, 200 mg/mL of MNPs, 0.3% cross linking with carbodiimide, and 3 h of cross-linking time. The magnetization activity of MNPs (8 emu/g) and the immobilized laccase with MNPs (4 emu/g) was analyzed using vibrating sample magnetometer (VSM). Maximum activity of immobilized laccase was observed at pH 7.0 and 55 °C. The immobilized laccase has greater stability (100 h) and significant chlorpyrifos (pesticide) degradation activity. High-performance liquid chromatography (HPLC) results confirmed the degraded metabolic products of chlorpyrifos. In all, the immobilized laccase was superior to free laccase, showing promising structural and application characteristics. [ABSTRACT FROM AUTHOR]

Lignocellulosic agricultural bi-products, pearl millet (PM) and finger millet (FM) husks, were used for the production of laccase using Bacillus sp. PS under solid-state fermentation (SSF). Abiotic variables such as substrate (PM, FM) concentration (1–5%), incubation time (24–96 h) and pH (5–10) were optimized using Response surface methodology (RSM) to maximize the laccase production. The predicted model showed maximum laccase activity of 402 U/mL appearing after 96 h of incubation with PM 2.0 g/L and FM 1.5 g/L at pH 7.0. Single protein band on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) confirmed homogeneity of the laccase with a molecular weight of 63–75 kDa. The partially purified laccase effectively degraded the pesticides (Tricel, 71.8 ± 3.5 and Phoskill 77.3 ± 3.4%) within 5 days of incubation (40 °C) in pH 7.0. The pesticide degradation was further confirmed by high-performance liquid chromatography (HPLC) and the chromatograms showed the single dominant peaks at retention time 2.482 (tricel) and 2.608 (phoskill) min, respectively. Pesticide-degrading laccase was produced by Bacillus sp. PS under SSF reveals the utilization of low-cost bi-substrates for enhanced laccase production. [ABSTRACT FROM AUTHOR]

The present study explores the rapid chlopyrifos (CPs) biodegradation potential of plant growth promoting (PGP) psychrophilic bacteria isolated from brackish water by enrichment culture technique. Based on biochemical tests and 16 S rDNA sequencing the isolate was identified as Shewanella sp. The isolate Shewanella BT05 showed significant growth rate in various concentrations of (10–50 mg/L) CPs. The isolate produced plant growth promoting factors, IAA (20.8 ± 1.2 and 15.4 ± 1.0 μg/mL) and siderophores (60.67 ± 1.2 and 57.5 ± 0.9%) in the absence and presence of CPs. Further, the isolate BT05 solublized phosphate (16.5 ± 1.0 and 12.0 ± 1.0 mm in size respectively), and produce hydrogen cyanide (excellent and moderate) in the presence and absence of CPs. The isolate BT05 degraded 94.3, 91.8, 87.9, 82.6, and 80.5% of CPs at 10, 20, 30, 40, and 50 mg/L, respectively, within 24 h. Further, the media conditions were optimized for enhanced CPs removal and observed 93% removal in the presence of 3.5% glucose in pH 7.0 at 32.5 °C. Fourier-transform infrared spectroscopy and high-performance liquid chromatography results indicated the role for Shewanella BT05 in the biomineralization of CPs. The results suggested the isolate BT05 could be used for CPs removal as well as PGP activity in contaminated soil. • The isolate Shewanella sp. BT05 showed rapid degradation of CPs (94.3%) within 24 h. • Optimization studies showed that 13% increased CPs removal (93%) in the presence of 3.5% glucose, pH 7.0 and 32.5 °C. • 3, 3,6-trichloro-2-pyridinol (TCP), metabolic product was observed in the CPs treated with Shewanella sp. BT05. [ABSTRACT FROM AUTHOR]