This paper presents experimental and numerical studies to investigate the fire behavior of high strength steel (Q690 and Q960) shear connections. The load-displacement curves, failure modes, and load capacities, including the slip load and ultimate load of the specimens at elevated temperatures were obtained from experiments. A finite element model was established by ABAQUS software and validated by the test results to investigate the effect of the friction coefficient and the assembly form of the bolt on the shear behavior. The numerical results of load capacity were compared with that predicted by EC3, AISC 360–16, and GB50017. Parametric studies were conducted to investigate the effect of the slip load on the ultimate load at ambient temperature, and design methods were proposed considering the friction coefficient and preload values. The research shows that the slip load decreases more rapidly than the ultimate load at elevated temperatures. The critical temperature from brittle failure to ductile failure is approximately 500 °C. The load capacity predicted by AISC 360–16 is accurate, while EC3 and GB50017-2017 predict conservative load capacity. The effect of the bolt load and friction coefficient cannot be ignored for high strength steel shear connections. In order to investigate the fire behavior of high strength steel (Q690 and Q960) shear connections, experimental and numerical studies were presented in this paper. The load-displacement curves, failure modes, and load capacity, such as the slip load and ultimate load of the specimens at elevated temperature were obtained from the experiment. Finite element model was established by ABAQUS software and validated by the test results to investigate the effect of the friction coefficient and the assemble form of the bolt on the shear behavior. The analytical results of load capacity were compared with that predicted by EC3, AISC 360–10, and GB50017. Parametric studies were conducted to investigate the effect of the slip load on ultimate load at ambient temperature, and design methods were proposed considering the friction coefficient and preload values. The research shows that the slip load decreased more rapidly than the ultimate load. The failure temperature from brittle failure to ductile failure is approximate 500 °C. The stiffness of the connection deceases significantly due to the eccentric assembly form of the bolt. The load capacity predicted by AISC 360–10 is accurate, while EC3 and GB50017-2017 predict conservative load capacity. [Display omitted] • Tests on shear capacity of high strength steel connections at elevated temperature were carried out. • Finite element models on shear connection were established and validated by test data. • Parametric studies were carried out to quantitatively assess the influence of key factors on shear capacity of connection. • The shear load capacity of connection predicted by AISC 360–10,EC3 and GB50017-2017 were evaluated. [ABSTRACT FROM AUTHOR]