Search

BOWEN'S DISEASE (BD) IS AN IN SITU, PREINVASIVE CANCER WITH POTENTIAL FOR PROGRESSION IN SQUAMOUS CELL CARCINOMA (SCC), BEING CLINICALLY CONSIDERED A PRECANCER WITH A HIGH POTENTIAL FOR MALIGNANCY. THE AIMS OF THE STUDY WERE TO ASSESS THE CLINICAL, HISTOLOGICAL, AND EVOLUTIONARY ASPECTS OF THE BOWEN'S DISEASE AND INVASIVE BOWEN DISEASE. THE STUDIED GROUP COMPRISES 50 PATIENTS DIAGNOSED WITH BOWEN DISEASE OR INVASIVE BD, HOSPITALIZED IN THE DERMATOLOGY CLINIC OF EMERGENCY COUNTY HOSPITAL OF CRAIOVA, BETWEEN 2010 AND MARCH 2021. THERE WERE 18 MALES AND 32 FEMALES, AGED 30 TO 85 YEARS, 66% OF THE PATIENTS BEING FROM THE URBAN ENVIRONMENT. THE MOST FREQUENT LOCALIZATION OF BD WAS AT THE LEVEL OF THE LIMBS (52%), ON PHOTOEXPOSED AREAS. IN 92% OF CASES, WE NOTICED CLINICAL ASPECT OF BD WITH SINGLE LESIONS AND THE TYPICAL FORM WAS PREDOMINANCE (80%). THE PATHOLOGY DIAGNOSED 41 BD AND 9 INVASIVE BD (SQUAMOUS CELL CARCINOMA). PRESUMPTIVE CLINICAL DIAGNOSIS IN 31 CASES (57.40%), WAS CONFIRMED BY HISTOPATHOLOGICAL EXAMINATION. THE CORRELATION OF CLINICAL, HISTOPATHOLOGICAL DATA LEAD TO AN ACCURATE DIAGNOSIS OF BD. BOWEN'S DISEASE, ALONG WITH OTHER KERATINOCYTIC PRECANCERS, BEING A STRONG PREDICTOR OF THE RISK OF DEVELOPING SKIN CARCINOMAS AND MELANOMA, REQUIRING A PROMPT THERAPEUTIC ATTITUDE AND CAREFUL MONITORING. [ABSTRACT FROM AUTHOR]

Copyright of Romanian Journal of Clinical & Experimental Dermatology is the property of SC Media Systems Communication SRL and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The tumor microcirculation is characterized by an abnormal vascular network with dilated, tortuous and saccular vessels. Therefore, imaging the tumor vasculature and determining its morphometric characteristics represent a critical goal for optimizing the cancer treatment that targets the blood vessels (i.e. antiangiogenesis therapy). The aim of this study was to evaluate new vascular morphometric parameters in colorectal cancer, difficult to achieve through conventional immunohistochemistry, by using the confocal laser endomicroscopy method. Fresh biopsies from tumor and normal tissue were collected during colonoscopy from five patients with T3 colorectal carcinoma without metastasis and were marked with fluorescently labeled anti-CD31 antibodies. A series of optical slices spanning 250 µm inside the tissue were immediately collected for each sample using a confocal laser endomicroscope. All measurements were expressed as the mean ± standard error. The mean diameter of tumor vessels was significantly larger than the normal vessels (9.46±0.4 µm vs. 7.60±0.3 µm, p = 0.0166). The vessel density was also significantly higher in the cancer vs. normal tissue samples (5541.05±262.81 vs. 3755.79±194.96 vessels/mm3, p = 0.0006). These results were confirmed by immunohistochemistry. In addition, the tortuosity index and vessel lengths were not significantly different (1.05±0.016 and 28.30±3.27 µm in normal tissue, vs. 1.07±0.008 and 26.49±3.18 µm in tumor tissue respectively, p = 0.5357 and p = 0.7033). The daughter/mother ratio (ratio of the sum of the squares of daughter vessel radii over the square of the mother vessel radius) was 1.15±0.09 in normal tissue, and 1.21±0.08 in tumor tissue (p = 0.6531). The confocal laser endomicroscopy is feasible for measuring more vascular parameters from fresh tumor biopsies than conventional immunohistochemistry alone. Provided new contrast agents will be clinically available, future in vivo use of CLE could lead to identification of novel biomarkers based on the morphometric characteristics of tumor vasculature. [ABSTRACT FROM AUTHOR]

We present a patient, treated for 3 months with clomiphen citrate after 5 years of infertility. This treatment resulted in a twin pregnancy, one degenerated into a partial hydatidiform mole and the other into a very early embryo death. The karyotype was a mosaic one: 63% of metaphases showed triploidy - 69 XXX and 37% diploidy - 46 XX. Despite all medical advice, she returned 8 months later with a new pregnancy, which proved to be a new partial hydatidiform mole, this time a single one. Karyotype was, also, a triploidy - 69 XXX. The genetic map of both genitors was performed, showing no aberrations. Unfortunately, the patient came back, once again, 5 months later, with a new positive pregnancy test. Ultrasonography revealed a new very early embryo death, the histopathological analysis establishing to be a single 'pure' stop in evolution of the pregnancy. As all the three pregnancies obtained after treatment with clomiphene were abnormal, two being partial hydatidiform moles and one being a premature miscarriage, without any genetic aberrations of the genitors, it seems very possible that clomiphene, apart from improving fertility, also increases the risk of abnormal ovum appearance. [ABSTRACT FROM AUTHOR]

Confocal laser endomicroscopy (CLE) has been recently proposed as a new technique that allows in vivo histologic assessment of mucosa during endoscopy. The most commonly used contrast agents are acriflavine hydrochloride and fluorescein sodium. For colon pathology assessment, the administration of fluorescein intravenously produces a strong staining of both surface epithelium and deeper layers of lamina propria. Confocal laser endomicroscopy is a feasible method to diagnose colon cancer in vivo. Furthermore, confirmation of neoplastic changes using CLE during colonoscopy may lead to major improvements in the clinical management of the patients with inflammatory bowel disease. Biopsies can be limited to targeted sampling of relevant lesions. Confocal laser endomicroscopy will certainly play an important diagnostic role during gastrointestinal endoscopy in the future, enabling the elimination of the diagnostic delay associated with conventional biopsy preparation and processing. [ABSTRACT FROM AUTHOR]