1. Evaluation of anti-AIDS drugs in conventional mice implanted with a permeable membrane device containing human T cells infected with HIV.
- Author
-
Quenelle DC, Keith KA, Dunleavy KE, Taylor BA, Bowdon BJ, Brazier AD, Mullon CJ, HarrisRE, and Allen LB
- Subjects
- Acrylic Resins, Animals, CD4-Positive T-Lymphocytes cytology, CD4-Positive T-Lymphocytes transplantation, Cell Survival, Cytopathogenic Effect, Viral, HIV Core Protein p24 analysis, HIV Reverse Transcriptase metabolism, HIV-1 growth & development, HIV-1 metabolism, Humans, Mice, Permeability, Polymers, Polyvinyl Chloride, Prostheses and Implants, Zidovudine pharmacology, Anti-HIV Agents pharmacology, CD4-Positive T-Lymphocytes virology, Drug Evaluation, Preclinical methods, HIV-1 drug effects, Membranes, Artificial
- Abstract
We now report the confirmation of the work of Hollingshead et al. (1995) on development of a cell based hollow fiber (HF) system for evaluating potential anti-AIDS drugs in vivo using conventional mice rather than SCID mice. CD4 +, CEM-SS cells infected with HIV/1, strain RF, at a multiplicity of infection of 0.1 were placed into HFs. The fibers were implanted into the peritoneal cavity of outbred Swiss mice. Using this model, the antiviral activity of azidothymidine (AZT) at doses of approximately 150, 75 and 37.5 mg/kg/day was evaluated by administering AZT to the mice in drinking water. Upon fiber removal on day 6, AZT treatment was shown to significantly increase CEM cell viability over the untreated, virus control group and significantly reduced the levels of HIV p24 and HIV RT activity.
- Published
- 1997
- Full Text
- View/download PDF