1. Evolution of total and integrated HIV-1 DNA and change in DNA sequences in patients with sustained plasma virus suppression.
- Author
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Izopet J, Cazabat M, Pasquier C, Sandres-Sauné K, Bonnet E, Marchou B, Massip P, and Puel J
- Subjects
- Amino Acid Sequence, DNA, Viral blood, Genes, env genetics, Genes, pol genetics, HIV Envelope Protein gp120 genetics, HIV Infections drug therapy, HIV Protease genetics, HIV Reverse Transcriptase genetics, HIV-1 drug effects, HIV-1 genetics, Humans, Molecular Sequence Data, Mutation, Peptide Fragments genetics, Proviruses, Sequence Analysis, DNA, Antiretroviral Therapy, Highly Active, DNA, Viral genetics, Drug Resistance, Viral genetics, Evolution, Molecular, HIV Infections virology, HIV-1 classification
- Abstract
Blood samples from patients with plasma HIV-1 RNA <20 copies/ml for more than 2 years were studied. Significant decreases in total and integrated HIV-1 DNA were observed during the first 15 months of suppressive therapy before the concentrations became stable. Clonal analysis of HIV-1 pol demonstrated that the proportions of resistance mutations in DNA sequences after 2 years were lower than those in baseline DNA and RNA sequences. The changes in the clonal composition of HIV-1 env populations in three patients with evidence of changes in HIV-1 pol populations indicated a shift from predominantly R5-like viruses to predominantly X4-like viruses in two patients and the persistence of predominantly X4-like viruses in the third. Our analyses indicate the reemergence of ancestral sequences from long-lived cells or the residual production of wild-type virus from anatomic sites with limited access to antiretroviral drugs and the preferential infection of cells expressing CXCR4.
- Published
- 2002
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