1. The light chain of antibodies specific to the V2 region of HIV-1 can determine their function.
- Author
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Li L, Wang XH, Nanfack A, Kong XP, and Gorny MK
- Subjects
- Adult, Female, HIV-1 genetics, Humans, Male, Antibodies, Monoclonal genetics, Antibodies, Monoclonal immunology, HIV Antibodies genetics, HIV Antibodies immunology, HIV Infections genetics, HIV Infections immunology, HIV-1 immunology, Immunoglobulin Light Chains genetics, Immunoglobulin Light Chains immunology, Immunoglobulin Variable Region genetics, Immunoglobulin Variable Region immunology
- Abstract
We studied the contribution of the light chain to functions of human monoclonal antibodies (mAbs) by measuring the relationships between the rate of mutations and cross-reactivity, binding affinity and neutralization activity. We analyzed 12 mAbs of two clonal families specific to the V2 region of HIV-1 derived from two chronically HIV-1 infected individuals. The clonal mAbs exhibited a range of reactivities, and the clones with superior properties were associated with the rate of mutations and the presence of particular mutated residues in the light chains, but not in the heavy chains. Our observations suggest that for some antibodies, the light chains play a vital role in antibody evolution toward more efficient ones and also suggest the importance of optimal residues rather than the rate of mutations in the variable fragment of the antibody., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Published by Elsevier Inc.)
- Published
- 2021
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