Search

Your search keyword '"Kutuzova GD"' showing total 2 results
Start Over Author "Kutuzova GD" Topic horseradish peroxidase
2 results on '"Kutuzova GD"'

1. [Inhibition of horseradish peroxidase by N-ethylamide of o-sulfobenzoylacetic acid].

Author

Rogozhin VV, Kutuzova GD, and Ugarova NN

Subjects
Acetates chemistry, Benzenesulfonates chemistry, Horseradish Peroxidase antagonists & inhibitors, Horseradish Peroxidase chemistry
Abstract

The carboxylic groups of horseradish peroxidase were modified by 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate by the Koshland method. The catalytic properties of the native and modified peroxidase were studied in the presence of N-ethylamide of o-sulfobenzoylacetic acid (EASBA) at pH 5.0-7.5. In the oxidation of o-dianisidine, EASBA is a competitive inhibitor of the carbidiimide-modified peroxidase, and it increases both K(m) and Vm in the case of the native enzyme. These data show that at least one of the carboxylic groups modified with carbodiimide is located at the area of the peroxidase active site.

Published
2000

2. [Functionally important carboxyl groups of horseradish peroxidase].

Author

Kutuzova GD, Rogozhin VV, Ugarova NN, and Berezin IV

Subjects
Binding Sites, Carboxylic Acids, Catalysis, Enzyme Activation drug effects, Guanidines metabolism, Hemin metabolism, Hydrogen-Ion Concentration, Structure-Activity Relationship, Substrate Specificity drug effects, Horseradish Peroxidase metabolism, Peroxidases metabolism
Published
1983

Catalog

Books, media, physical & digital resources
See catalog results