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Winter hardiness may be influenced by vineyard terroir-driven factors, and vineyard zones with low water status [leaf water potential (ψ)] could be more winter hardy than vines with high water status (higher leaf ψ). Six Cabernet franc vineyards were chosen throughout the Niagara region in Ontario. Data were collected at fruit set, lag phase, and veraison [soil water content (SWC), leaf ψ], at harvest (yield components, berry composition), and three times during winter (LT50; the temperature at which 50% of buds die) in the 2010–2012 seasons. Interpolation by kriging and mapping of variables was completed using ArcGIS, and statistical analyses (linear correlation, k-means clustering, principal components analysis, multilinear regression) were performed. Spatial trends were observed in each vineyard for SWC, leaf ψ, yield components, berry composition, and LT50. Geographic information systems (GIS) and statistical analysis revealed that leaf ψ could predict LT50, with strong positive correlations between LT50and leaf ψ values in most vineyards in 2010–2011. In the dry 2012 season, leaf ψ (particularly at veraison; range −1.3 to −1.6 MPa) was positively correlated to LT50, yield, titratable acidity (TA), pH, and Brix and negatively to SWC, color, anthocyanins, and phenols. Overall, vineyards in different appellations (Niagara Lakeshore, Lincoln Lakeshore, Four Mile Creek, Beamsville Bench) showed many similarities. These results suggest that there is a spatial component to winter injury, as with other aspects of terroir. Furthermore, this study allows for means by which to compare winter hardiness to other critical variables to better understand the terroir of the Niagara region.

Vineyard replanting in Washington state can be negatively impacted by the plant-parasitic nematode Meloidogyne hapla. Chemically focused nematode management programs do not offer long-term suppression; however, this may be achieved through the adoption of cultural approaches such as rootstocks and irrigation. Nematode-resistant rootstocks are used extensively in other regions but many have not been tested against M. hapla. Vineyards in eastern Washington are irrigated; therefore, manipulating available soil water may also impact nematode development. In 2017, two field trials were established in eastern Washington to evaluate the effects of (i) late-summer water limitation on M. hapla population development and (ii) host status of 1103 Paulsen, 3309 Couderc, and Matador rootstocks for M. hapla. The efficacy of these cultural management approaches was evaluated under three initial M. hapla densities (0, 50, and 250 M. hapla second-stage juveniles per 250 g of soil) in both trials. Reducing irrigation to manage M. hapla infestation of grape roots was ineffective and may cause harm to the vines by inducing too much water stress. Conversely, rootstocks effectively reduced population densities of M. hapla. Overall, rootstocks show the most promise as a cultural tool to manage M. hapla during the establishment phase in Washington vineyards.

TLow yeast assimilable nitrogen (YAN) concentrations ( 140 mg/N/L) levels. Irrigation had no impact on YAN concentrations. Treatment influences on vine vegetative growth, yield, and grape and wine composition were marginal and inconsistent, and were largely influenced by climatic conditions. Cool-climate grape growers would benefit from applying more N in the vineyard around veraison to improve YAN, without stimulating vigor and negatively impacting chemical grape and wine composition. Increasing irrigation rates may be advantageous in seasons of high crop load, however current commercial irrigation rates are considered adequate.

Characterisations of thirty grapevine varieties (Vitis vinifera L.) from the experimental vineyard ‘Radmilovac’ were conducted using a large number of OIV descriptors and eight highly polymorphic microsatellite loci. The ampelographic description contained 45 features. Molecular characterisation of selected microsatellite loci was performed using capillary electrophoresis fragment analysis. Dendrograms based on ampelographic and genetic data resulted in three groups of varieties. Qualitative ampelographic characteristics tended to manifest significant differences. The most common deviation among varieties within the group was in the characteristic OIV 051 (colouration of the upper side of a young leaf). Genetic characterisation of SSR markers through analyses of a large number of varieties contributes to better organisation of grapevine collections and simpler identification of varieties, as well as data exchange. When identifying the varieties, the results of the DNA analysis should be combined with the ampelographic descriptors, in order to select grapevine varieties with desirable viticultural and oenological traits. Integration of the obtained genetic data with the ampelographic data is of utmost importance for accurate identification of the varieties and offers a significant means for the preservation and use of the varieties.

The research aimed to study the combined effect of inter-row sodding with perennial grasses and biofertiliser applications (microbial preparations, MPs) on soil fertility and biological activity, as well as on mineral nutrition, productivity and quality of grapes. The experiment was conducted at a vineyard of the Shasla x Berlandieri 41B-rootstock Muskat Belyy variety nearby Sevastopol. The two-factor design was as follows. Sodding: with segetal vegetation (SV) or a mixture of seeded cereal and leguminous herbs (MHs). Microbial preparations: grapevine root system and soil bacterisation with MPs of various action, including Diazophyte nitrogen fixer, Phosphoenterin (PE) phosphate-mobiliser and a complex of microbial preparations (CMP) additionally containing Biopolycide bioprotector. In control, MPs were not applied with SV or MH sodding. Preparations were introduced in soil once a year prior to grapevine flowering at a dose of 200 g MP suspension per bush. Herbs were mown 5–6 times per season at 30–40 cm height. A combined sodding—MPs usage has been found to increase the nitrate content by 24–45, mobile P2O5 – by 16–21, exchangeable K2O – by 28–50 and organic matter – by 0.06–0.13 % relative to control. The greatest increase in N-NO3–K2O content was registered for the combination of MHs, Diazophyte and CMP; combined SV—CMP–MHs had the greatest impact on mobile P2O5 and organic matter. Biologisation enriched mineral grapevine nutrition, especially for P and K, as well as increased the bush productivity by 10–14 % (maximum in CMP–MHs) via improving the berry and bunch mass and grape quality via significantly increasing the sugar content and lowering acidity of wort. MHs–CMP maximized counts of agronomically valuable microorganisms involved in the organic and mineral nitrogen and phosphorus turnover (ammonifiers and oligotrophs by 120–130, amylolytics and phosphate mobilisers by 50–70 and oligonitrophilic by 50– 80 %). All usages contributed to soil enrichment with nutrients and humus.

Global atmospheric carbon dioxide concentrations will continue increasing throughout the next century, with profound impacts on agriculture. The literature concerning the effects of climate change on viticulture has largely focused on the isolated impacts of variables such as temperature and soil water deficit. Likewise, the research on the effects of elevated atmospheric CO2 on grapevines is stunted at the categorical level, chiefly because of the difficulty of experimentally controlling the gaseous environment in situ for the years necessary to replicate the vineyard system in a future climate condition. Despite numerous studies on the short-term influence of environmental and cultural factors on grapevine development at elevated carbon dioxide, the long-term impacts remain poorly understood. The lack of field based elevated CO2 experiments in the United States is an added challenge to predicting viticultural changes, particularly in California. This review focuses on the systemic impact of atmospheric CO2 on Vitis vinifera, synthesizing physiological, phenological, and plant-pest interactions. Major findings from this synthesis inform of a predicted increase in pest pressure, advanced phenological timing, transient increase in water use efficiency for grapevine, and changes in grape berry chemistry. While water use efficiency is highly desirable, the prediction for current winegrape growing regions is a transient increase in water use efficiency subsequently limited by a lack of available soil water. Grapevine is influenced by the negative synergistic effects of heat, drought, and elevated CO2, which will alter cultural practices including harvest and pest/disease control, with downstream effects on winemaking. Several options for adaptation are discussed including leaf removal, planting alternative varieties and selective breeding of new varieties.

Background and Aims Knowledge of varietal wine flavour and aroma compounds has improved, but gaps exist concerning how grape composition impacts wine style. This work aimed to explore the influence that different grape tissues can have on the volatile profiles of wines. Methods and Results Riesling and Cabernet Sauvignon berries were separated into skin, flesh and seeds. Two sets of fermentations were performed using separated tissues: one using an equal mass of each tissue and another where the amount of each tissue in 25 g of berries was fermented. When an equal mass of tissue was used, the seed-derived wines had a higher concentration of esters than that produced from other grape tissues. Those produced using skins had the highest concentration of lipoxygenase pathway-derived compounds, and, for Riesling, a higher concentration of monoterpenes. When the proportional amounts of each tissue found per berry were used, the flesh-derived wines generally had a higher concentration of many wine volatiles compared to the other tissues. This reflects the greater proportion of flesh tissue in the berry compared to skin and seeds. Conclusions Seed-derived compounds can enhance ester biosynthesis during fermentation and skins appear to have high lipoxygenase pathway activity. Nevertheless, the flesh makes up such a large proportion of the whole berry that it has the major influence on volatile profiles of whole berry fermentations. Significance of the Study Different berry tissues can alter wine composition in unique ways, and this can inform strategies to alter wine composition through vineyard management or the selection of new germplasm.

Phomopsis cane and leaf spot (PCLS) is an important disease of grapevines that is mainly caused by Diaporthe ampelina. Dispersal dynamics of D. ampelina spores were investigated in two vineyards, one in northern Italy and one in Montenegro, by using spore samplers that collected α- and β-conidia from rain water running off from PCLS-affected canes. The canes were collected from each vineyard, deployed, and overwintered in the corresponding vineyards. In each of three years (2016, 2017, and 2018), conidial dispersal was investigated during one (Montenegro) or two (Italy) growing seasons following the deployment of the PCLS-affected canes. In the first growing season following cane deployment in both vineyards, α-conidia were mostly found in runoff water after grapevine bud break, especially in April and May, and β-conidia were regularly found in numbers comparable to those of α-conidia, most frequently from June to September. In Italy, high numbers of α- and β-conidia were also collected during the second growing season following cane deployment. The dispersal dynamics of α-conidia over time were described by a Gompertz equation using hydrothermal time (i.e., the accumulated effect of temperature on the maturation rate of pycnidia on days in which the number of hours of wetness was ≥6 or 9 h), with R2 and concordance correlation coefficient >0.9. Rain (≥0.2 mm) was a good predictor of conidial dispersal, with an overall accuracy of 0.97. These results increase our understanding of D. ampelina spore dispersal and should be integrated into warning systems for PCLS management.

The present research focused on the characterisation of climate evolution in a typical Apulian region for table grape production under the protected geographical indication, “Uva di Puglia I.G.P.”Two thirty-year time window period (TW) were analysed: 1961-1990 and 1991-2020. Georeferenced maps for both TWs were produced to delimit homogeneous zones and to evaluate the climate variability within the investigated area by means of the two bioclimatic indices, Heliothermal Index (HI) and Winkler Index (WI). Spatial analysis of HI and WI was performed using the regression-kriging (RK) interpolation method and the Digital Elevation Model/DEM (10 x 10 m) as a prediction attribute.An increase in both the minimum and maximum temperatures was observed, and locations above 300 m a.s.l. shifted from HI+1 “temperate warm” to HI+2 “warm” according to the Geoviticulture Multicriteria Climatic Classification System. WI values similarly increased between the periods 1961–1990 and 1991–2020, shifting all the sites grouped in the Elevation Classes defined as being below 300 m a.s.l. from Region IV to Region V of the Winkler Classification.According to HI and WI, presumed maturity was calculated as being reached 9 to 15 (HI) and 12 to 28 days (WI) earlier in 1991–2020 than in 1961–1990, taking into account the heat requirements of cv. Italia table grape (representative of Apulian table grape production), were set at 2200 for both indices on the basis of literature data.Moreover, three table grape vineyards, located in the three main producing provinces of Apulia (Bari, Taranto and Barletta-Andria-Trani (BAT)), were considered for future scenarios analysis on the basis of two different Representative Concentration Pathways (RCPs), 4.5 and 8.5, and classified according to the Geoviticulture Multicriteria Climatic Classification System (MCC). Future scenarios scored WI values that exceeded the threshold of 2700 in the BAT and TA provinces in the 2061–2090 time window period for RCP 8.5. In contrast, RCP 4.5 led to a mitigating effect, which was not noticeable until 2040, with a consequent reclassification of the investigated areas on the basis of HI and Cool Night Index (CI).These findings suggest that in order to prevent or overcome heat stress, it will be necessary to implement strategies, such as vineyard relocation to unexplored elevations or latitudes and/or the exploitation of new table grape varieties able to fulfill the optimal maturity parameters, even when the duration of the phenological phases is shorter.

In table grapes (cv. Thomson Seedless), the antifungal activity against Botrytis cinerea was further analysed and compared between two high potential bacterial biological control agent (BCA) strains, Bacillus velezensis BUZ-14 and B. ginsengihumi S38. Two commercial biocontrol products (BPs), served as standards of comparison, Amylo-X® and Serenade Max®, also based on Bacillus BCA strains. The main mode of action quantified for all the strains was antibiosis due to hydrosoluble and volatile metabolites and their combinations. The BUZ-14 strain was the most active BCA strain, demonstrating significant disease reduction exceeding 60 % when used in the culture form grown in 863 medium, including living cells (LCs) and cell-free supernatant (CFS). Both BPs exhibited significantly reduced efficacy of their CFS fraction (< 10 %) compared with that of the two BCA strains, confirming their high antibiosis potential. The novel methodology allowed us to demonstrate the significant effect of the BCA culture medium on volatilome (VOC) antagonist efficacy. The S38 strain achieved the highest disease reduction (90 %) owing to the greatest production of VOCs in the richest MOLP (Medium Optimum Lipopetide Production) culture medium, whereas grape juice was the least favourable medium for VOC efficacy for both bacterial strains (BUZ14 and S38). The overall poor activity of living Bacillus cells in all the BCA and BPs tested is discussed based on the low capacity of the BCA strains to grow in the berry. Then, the presence of living cells is also discussed with the possibility that these cells are not required in field applications of such BCA strains in this genus. Moreover, different environmental suboptimal conditions, including temperature (22 and 27 °C) and relative humidity (RH) (100 and 85–95 %), were tested, and BUZ-14 exhibited the highest Botrytis reduction at both temperatures and RH values. However, no significant differences were observed between temperatures or RH values for the same BCA. Further studies in vineyard conditions and applications, such as biofumigation or active packaging, will be performed to confirm the new findings reported in this investigation.

‘Xinomavro’ (the second planted red Greek variety behind ‘Agiorgitiko’) generally produces wines that are light in colour but with increased astringency, possibly related to grape flavonoid amount and composition; although irrigation is accepted as an effective means to enhance grape phenolic maturity, its role has not yet been sufficiently studied in the case of Xinomavro. This study aimed to determine the effect of post-veraison irrigation on berry anthocyanin and proanthocyanidin amount and composition, of field-grown Xinomavro vines (Vitis vinifera L.), under the typical summer conditions of Northern Greece. In a 10-year-old Xinomavro vineyard, two post-veraison watering regimes were applied—irrigation starting 20 days after veraison (mid-ripening irrigation, MRI) and irrigation starting immediately after veraison was completed (after veraison irrigation, AVI)—alongside non irrigated vines (NI), and vines irrigated continuously from berry set through harvest (continuous irrigation, CI). Proanthocyanidin composition was determined in both skins and seeds by employing phloroglucinolysis followed by HPLC-UV and MS detection (high-performance liquid chromatographic with tandem mass spectrometric and ultraviolet absorbance detection), and the anthocyanin profile was identified only in the skin extracts by HPLC-UV (high-performance liquid chromatographic with ultraviolet absorbance detection). Post-veraison irrigation increased yield parameters and reduced anthocyanin levels and the proportion of their stable forms (acylated vs. non-acylated, tri-oxygenated and methoxylated on the B-ring vs. di-oxygenated and hydroxylated), compared to NI vines; however, these effects were more pronounced in the case of early post-veraison irrigation (AVI) than late-season irrigation (MRI). Irrigation also increased the mean Degree of Polymerization (mDP) and prodelphinidin percentage of skin tannins and decreased mDP of seed tannins. In the light of the necessity to control the accelerated ripening under the increasingly hotter and drier climatic conditions, late irrigation (MRI) might provide a solution to avoid excessive sugar levels and allow a slightly higher yield without significant reductions in berry phenolic content. The results suggest that the optimisation of the timing of irrigation could provide an effective adaptation strategy to climate change in Mediterranean viticultural areas.

The current study aimed to screen glyphosate-alternative weed control methods in three perennial crops in Greece. Field trials were conducted and repeated (2018 to 2019 and 2019 to 2020) in a citrus orchard (Citrus clementina Hort. ex Tan), an olive grove (Olea europaea L.), and a vineyard (Vitis vinifera L.) under the randomized complete block design (nine treatments, four blocks). Glyphosate was applied in the citrus orchard (720 g ae ha−1), the olive grove (720 g ae ha−1), and the vineyard (1,800 g ae ha−1). Pelargonic acid (1,088 g ha−1; two times), barley (Hordeum vulgare L.) residues and white mustard (Sinapis alba L.) residues were evaluated in all sites. Mowing was evaluated in the citrus orchard (one time) and the vineyard (two times). Flazasulfuron (50 g ha−1), oxyfluorfen (144 g ha−1), and flumioxazin (150 g ha−1) were applied in the citrus orchard and the olive grove. Penoxsulam + florasulam (15 + 7.5 g ha−1) was also applied in the olive grove. Cycloxydim (200 g ha−1), quizalofop-p-ethyl (150 g ha−1) and propaquizafop (150 g ha−1) were applied in the vineyard. An untreated control was included in all sites. Flazasulfuron, oxyfluorfen, and flumioxazin resulted in similar normalized difference vegetation index (NDVI) and weed biomass to glyphosate in the citrus orchard in both years and evaluations. Pelargonic acid (two times) and mowing (one time) were effective on broadleaf weeds. Flazasulfuron and penoxsulam + florasulam were the most promising glyphosate-alternative weed control methods against hairy fleabane [Conyza bonariensis (L.) Cronquist] in the olive grove. Cover crop residues showed their suppressive ability as in the citrus orchard. All selective herbicides resulted in similar NDVI and johnsongrass [Sorghum halepense (L.) Pers.] dry weight values in the vineyard in both years. Negative and strong correlations were observed in all sites and years between crop yield and weed dry weight (R2 = 0.543 to 0.924).

The choice of planting density is a key decision with long-term implications for grape growers to make before vineyard establishment. The field trial with drip-irrigated, machine-pruned Concord juice grapes described here tested the effects on yield formation and fruit composition of two between-row distances (2.44 m and 2.74 m) and four within-row distances (0.91 m, 1.83 m, 2.74 m, and 3.66 m), resulting in planting densities ranging from 997 to 4485 vines/ha. Canopy size, yield components, and fruit composition were measured over 6 years, starting in year 3 after planting. Whereas in the first cropping season the yield at 0.91 m and 1.83 m vine spacing (11.8 t/ha) was twice that at 2.74 m and 3.66 m (5.6 t/ha), on average over the 5 subsequent years the yield of 0.91-m vines was 38% lower (18.2 t/ha) than that at the other planting distances (29.2 t/ha). During the last 4 years, the average yield of vines planted at 2.44 m between rows was 2 t/ha higher than that at 2.74 m. The yield potential and fruit quality of closely spaced vines (0.91 m) was compromised by their vigorous growth, high canopy density, and poor microclimate, which resulted in fewer clusters/vine, fewer berries/cluster, lower cluster weights, and more bunch-stem necrosis. Leaf death in the canopy interior was associated with nutrient remobilization and high potassium and pH in the juice from 0.91-m vines. Juice total soluble solids, titratable acidity, and color remained unaffected by planting density. These results show that planting juice grapes at high density in irrigated and highly mechanized vineyards is detrimental to both cropping potential and fruit quality.

The present study was conducted to determine some biological criteria such as the first appearance of adults, peak date, and a number of generation for controlling honeydew moth in vineyard in Antalya province, Turkey during 2014 and 2015. In this study, the adult population was determined by pheromone traps and larval population was observed by counting the larvae on the grape clusters. It was determined that the pest adults were first seen in mid-April; however, the population was at very low levels until mid-June. It was demonstrated that the adult population of the pest could generate 4 peaks in June, July, August and September-October. The study results showed that the pest could be important in coastal vineyards. Besides, it has been showed that the effective temperatures sum based on the degree day model that was proposed in the literature could be employed to determine the honeydew moth adult and larval populations in vineyards.

Using detached leaves, UV-C light in the form of 1-s flashes has recently been shown to stimulate defenses of several plants against different pathogens better than 1-min exposures under greenhouse conditions. In the present work, the pathological tests were conducted using undetached leaves under greenhouse and vineyard conditions. In a first trial, two flashes of UV-C light were applied to plants of Vitis vinifera L. ‘Chardonnay’ grown under greenhouse conditions, at an interval of 10 days. Plants were inoculated with Erysiphe necator 2 days after the last light treatment. After 18 days of inoculation, the symptom severity on leaves was reduced by 60% when compared with the untreated control. In a second trial, flashes of UV-C light were applied to grapevine Chardonnay plants under field conditions in the southeast of France every 10 days from 18 April until 10 July 2019. The symptom severity resulting from natural contaminations by E. necator was reduced by 42% in leaves on 4 July 2019 and by 65% in clusters on 25 July 2019. In a third trial, we observed that UV-C light did not have any effect on net photosynthesis, maximal net photosynthesis, dark respiration, maximal quantum efficiency of photosystem II, the performance index of Strasser, and, generally, any parameter derived from induction curves of maximal chlorophyll fluorescence. It was concluded that flashes of UV-C light have true potential for stimulating plant defenses against E. necator under vineyard conditions and, therefore, help in reducing fungicide use.

Organically produced conventional products have gained great attention in accordance with the increasing awareness on healthy foods as well as improvements in living standards. Environment friendly strategies to extend the quality of grapes after commercial maturity provide healthy and profitable sustainability in market chain. In the present study, possibilities to maintain the quality of grape clusters on grapevines without any chemical treatments have been studied under vineyard conditions in temperate climate characteristics of Konya Province in Central Anatolia. Grape clusters of ‘Alphonse Lavallée’ and ‘Narince’, one of the most popular conventional grape cultivar in Turkey, were kept on the vines trained with bilateral cordon system. The vines were drip irrigated during the hot summer and the vine canopy was covered with black nets after veraison to protect from the birds, hail and climatic extremities. After commercial maturity, twelve cluster samples per cultivar were collected with monthly intervals to investigate the changes in quality. Up to the 3rd sampling date (at the end of November 2019), little losses in quality features were determined in both cultivars. At the end of the study, at 4th moth (December), significant decay incidences were observed with similar patterns in cultivars. General findings demonstrated that the grape clusters could be maintained on grapevines under intact condition of the cool autumn season in temperate zone by protecting them from the external damages using convenient protective materials up to three or four months depending on the response of cultivars to environmental variables.

This study was conducted to determine the pest species of Cicadomorpha and Fulgoromorpha (Hemiptera), distribution and densities in vineyards agroecosystems in Erzincan and Tunceli provinces. In determining the species, the impact method, sweep-net capture method, vacuum insect collector (d-vac) method were used. Surveys were carried out at regular intervals (approximately once a month) in vineyard areas in Erzincan and Tunceli provinces. As a result of this study, a total of 28 pest species belonging to Aphrophoridae (1), Delphacidae (2), Dictyopharidae (2), Membracidae (1) and Cicadellidae (22) families belonging to the Hemiptera order were determined in the vineyards of Erzincan and Tunceli provinces. Among them, it was determined that Empoasca solani (35.81%), Hauptidia cretacea (19.36%) and Psammotettix provincialis (8.49%), were the first degree harmful species in terms of distribution and density. In addition, 25 species other than these species were found to be secondary pests showing low density and distribution in the vineyards of Erzincan and Tunceli provinces.

An on-vineyard approach was used to investigate effects of the biodynamic preparations horn manure and horn silica (BD) on the soil structure in five vineyards on different bedrocks and that had be...

Grape mealybug consider the most universally important vineyard insect pest. Insecticides are the main strategy to grapevine insect control. The result showed that the recommended rate of Imidacloprid is succeeded in controlling of mealybug since its initial reduced of the infestation were 89.26, 84.21and 85.71 % for first season and 82.71, 82.94 and 83.56 % for second season for Flame seedless, Early sweet seedless and Sugraone seedless, respectively. Meanwhile, the total mean of reduction after 3,7, 14 and 30 days were 95.79, 64.83 and 72.82 % for first season and 85.20, 66.33 and 69.84 % for second season on Flame seedless, Early sweet seedless and Sugraone seedless, respectively. On the other hand, the total mean of reduction after 3,7, 14 and 30 days were 72.56, 80.40 and 82.50% for first season and 68.88, 76.13 and 75.56% for second season on Flame seedless, Early sweet seedless and Sugraone seedless, respectively. In addition, all of them are above 70% reduction. From these results, it should be suggested using some effective alternatives such as Palmito compound in controlling mealy bug incompatible program with chemical insecticides instead of conventional individuals' insecticides. The grapevine variety, Early sweet seedless showed the significant superiority in the quality traits. Under conditions of this investigation, the grapevine variety, Early sweet seedless was recorder the highest values for yield per vine, TSS and total sugar% in both seasons.

Monitoring the quality attributes of grapes is a practice that allows the state of ripeness to be checked and the optimal harvest time to be identified. A non-destructive method based on hyperspectral imaging (HSI) technology was developed. Analyses were carried out directly in the field on a ‘Sangiovese’ (Vitis vinifera L.) vineyard destined for wine production, by using a Vis/NIR (400–1000 nm) hyperspectral camera. One vineyard row was analysed on 13 different days during the pre-harvest and harvest time. The soluble solids content (SSC) expressed in terms of °Brix was measured by a portable digital refractometer. Afterwards, the grape samples were split in two classes: the first one composed by the samples characterised by a °Brix lower than 20 (not-ripe), while the second one by the samples with a °Brix higher than 20 (ripe). Grape mean spectra were extracted from each hyperspectral image and used to predict the SSC by partial least squares regression (PLS), and to classify the samples into the two classes by PLS discriminant analysis (PLS-DA). SSC was predicted with a R2 = 0.77 (RMSECV = 0.79 °Brix), and the samples were correctly classified with a percentage from 86 to 91%. Even if the number of wavelengths was limited, the percentages of correctly classified samples were again within the above-mentioned range. The present study shows the potential of the use of HSI technology directly in the field by proximal measurements under natural light conditions for the prediction of the harvest time of the ‘Sangiovese’ red grape.

The research was carried out in 2009, 2010, 2017, 2018 on the grape plantations of the experimental site of the Novocherkassk branch of the All-Russian Research Institute for Viticulture and Winemaking (Novocherkassk, Rostov region). In the vineyard of the Kunlean variety (rootstock Kober 5BB), planted in 2003 according to the 3x1.5 m scheme, the experiment provided for two options with bush shapes: a two-sided horizontal cordon and a two-sided horizontal cordon with a ground sleeve and a recovery knot. On the root-related plantings of grapes tolerant to phylloxera of the Pervenets Magaracha variety, planted in 1986 according to the same scheme, the forms of the bush were studied: a two-sided horizontal cordon, a long-sleeve and a two-sided horizontal cordon with a ground sleeve and a recovery knot. The load of shoots in all variants for both varieties was the same — 40 shoots per bush. The purpose of the research was a comparative analysis of the fruitfulness, yield and quality of grapes, depending on the shape of the bush. As a result of many years of research, it was revealed: on the Pervenets Magaracha variety, the highest rates of fertility (the share of fruit-bearing shoots is 82 %, K 1 — 1.45) and yield (14.0 t/ha) were in plantings with the form of bushes-a two-sided horizontal cordon with a ground sleeve and a recovery knot. The quality of the grapes was approximately equal in the variants with a long-sleeve and shaping and a two-sided horizontal cordon with a ground sleeve. The data on the annual growth of shoots were the lowest in the variant with a single-arm stem shape (shoot volume of 7.9 cm 3 , maturation of 44 %). On the Kunlean variety, the highest parameters of fruitfulness (the share of fruit — bearing shoots is 85 %, K 1 — 1.80), yield (27.0 t/ha) and the quality of berry juice (230 sugars and 7.8 g/cm 3 titrated acids) were in plantings with the form of bushes-a two-sided horizontal cordon with a ground sleeve. Data on the annual growth of shoots were also higher in this variant (shoot volume 35.0 cm 3 , aging 68 %).

Grapevine red blotch virus (GRBV) negatively impacts vine physiology and fruit quality in Vitis vinifera L. by reducing photosynthetic rate, total soluble solids (TSS), and berry anthocyanin concentration. Currently, growers have few management strategies beyond removal of infected vines, which may be particularly costly in vineyards with high disease incidence. The present study was established in 2018 in a GRBV-infected Pinot noir vineyard in southern Oregon to investigate whether reducing vine stress with cultural practices could dampen the impact of the disease on vine physiology and fruit quality. The effects of control and supplemental levels of irrigation and fertilizer on vine growth and physiology, disease severity, and fruit composition were observed over three years. Supplemental irrigation affected vine physiology and fruit composition in 2019 and 2020, but fertilization had no significant effect over three years. Photosynthetic rate, vegetative growth, vine yield, berry weight, TSS, and titratable acidity were increased with supplemental irrigation while disease severity (symptomatic leaves per vine) was reduced. Supplemental irrigation did not have consistent effects on secondary metabolites, though an increase in anthocyanin concentration was observed in 2020 despite an increase in berry size. Irrespective of applied water amounts, maintaining a higher vine water status effectively increased photosynthesis and canopy size that resulted in greater sugar accumulation. Ultimately, these results suggest that maintaining a high vine water status (Ψstem > -0.8 MPa) may mitigate some of the negative effects of GRBV on vine physiology and fruit composition.

Spring freeze events pose a threat to vineyard productivity worldwide. We compared two methods to delay grapevine budbreak for freeze avoidance and evaluated their effects on phenology, yield components, fruit composition, and postharvest parameters, including wine chemistry, carbohydrate storage, and bud freeze tolerance. The two methods to delay budbreak were: a vegetable oil-based adjuvant (Amigo®) applied to dormant buds at 8% and 10% (v/v) and late pruning applied when apical buds reached approximately Eichhorn-Lorenz stage 7. Treatments were applied in 2018 and 2019 on two Vitis vinifera cultivars, Lemberger and Riesling, and compared to a control treatment (no delayed budbreak strategy applied). Amigo and late pruning delayed budbreak compared to control vines for both years and cultivars. The delay in budbreak varied from three to six days for Amigo 8%, five to eight days for Amigo 10%, and 10 to 11 days later for late pruning. In 2019, there was a freezing event near budbreak; compared to control vines, late-pruned Lemberger vines had lower shoot damage when measured during the growing season and higher yield at harvest. Delayed budbreak treatments did not influence wine chemistry either year or consistently affect carbohydrate storage or bud freeze tolerance the following dormant season. However, in Riesling, late pruning reduced cluster and berry weight by up to 34% and 22%, respectively, compared to control vines. Furthermore, our results indicated Amigo 10% may decrease bud survival when applied to Riesling vines. In general, late pruning more effectively delayed budbreak and mitigated freeze damage than Amigo application without negatively affecting vine health and wine composition; however, the cultivar-dependent effect of late pruning on cluster weight is a consideration prior to adoption.

Vineyards have characteristic responses to sunlight. The reflectance values at the different wavelengths are typical of vegetation and define its spectral signature. The aim of this work was to determine the spectral signatures of table grape vineyards located in the production area of Robinvale (Victoria, Australia) in order to identify cultivars in the vineyard. Spectral signatures were detected from images in eight bands from the Worldview 3 platform. Spectral signatures for a representative number of plots were extracted using satellite reflectance images for each cultivar. Spectral signatures were also collected in the field, from the same cultivars, with a FieldSpec® HandHeld 2 portable spectroradiometer. Signatures were analyzed to determine the average spectral response for each plot and the variability in terms of coefficient of variation. Results showed that some cultivars exhibited different spectral signatures and trends while other cultivars had similar behavior. All the cultivars covered different regions of the Cartesian surface and were therefore distinguishable, except for ‘IFG 68-175’. Therefore, spectral signatures represent a powerful tool for a preliminary characterization of cultivars and vineyards. Spectral signatures were successfully used to classify vineyards down to the cultivar level and, within the same cultivar, to identify different management practices.

In the last decade, unmanned aerial vehicles (UAVs/UAS/RPAS) have been proposed for agricultural applications due to their great flexibility in flight scheduling and the increasing availability of dedicated miniaturized sensors. The capability of these systems in determining biophysical and geometrical parameters was tested in olive (Olea europaea L., ‘Frantoio’), vineyard (Vitis vinifera L., ‘Sangiovese’) and citrus orchards (Citrus clementina Hort. ex Tan. × Citrus sinensis L. Osb., ‘Mandared’) located at Venturina (LI, Suvereto (LI) and Lentini (SR), Italy, respectively. Images were acquired from an altitude of 50 m above ground level using an octocopter equipped with a consumer camera (RGB) and a multispectral camera (NIR-RG). The normalized difference vegetation index (NDVI) was calculated by means of the map algebra technique. Tree volume and canopy height were derived from the digital surface model (DSM) obtained from the images by UAV and structure from motion technique. Results showed that NDVI was linearly related to LAI (leaf area index) in all the tested species (R2=0.63-0.88). The best performance for tree canopy volume estimation was obtained in olive, with an R2 value of 0.90 and a slope of 0.85. The slight underestimation observed in the UAV-estimated volume of olive trees might be related with its irregularly shaped canopy, which is hardly assessed from the ground. The UAV-RGB camera technique was able to correctly estimate the canopy height of the three species (R2=0.79-0.88; RMSE=0.08-0.22 m). Our results confirm that the combined use of VIS-NIR cameras and UAV platforms is a rapid and reliable technique to determine canopy structure and LAI in orchards and vineyards.

The aim of this study was to test the effect of pre-flowering leaf removal (PFD) on grape sanitary status, yield, source-sink balance and berry composition. A completely random block design experiment was conducted over four seasons in a commercial vineyard of the Tannat/SO4 combination located in the south of Uruguay (34° 35´30 S, 56° 15´23 O). The PFD treatment was compared with a commercial defoliation (CC) comprising partial leaf removal between the fruit set and cluster closure stages (four leaves from the first node), a traditional practice carried out by winegrowers. Both treatments received equal phytosanitary management during the study. The results were conditioned by seasonal meteorological conditions, particularly rainfall, temperature and evapotranspiration. The seasonal effect was significant for yield components, berry composition and source-sink balance. The PFD treatment resulted in the lowest yields in all four years and modified the primary and secondary composition of berries. It also showed a higher anthocyanin potential in 2016, 2017 and 2019, related to a higher percentage of exposed clusters, lower yield and/or higher leaf to fruit ratio. In 2017, environmental conditions were more favourable for the development of bunch rot, and lower values of incidence (3 %) were recorded for the PFD treatment compared to CC (22 %), due to greater exposure of bunches and less compact bunches. The PFD vines showed a steady reduction in dry matter production capacity, mainly explained by the steady reduction in yields. The Tannat variety showed a differential response to PFD depending on the weather during the growth cycle. Under humid climate conditions, pre-flowering defoliation proved to be effective for yield control, by reducing bunch rot incidence, and for improving some attributes in terms of grape composition. This work provides useful information for grape growers in humid regions on how to improve the sanitary status and quality of their production.

This study was conducted during 2018, 2019 and 2020 seasons, on 10 years old Flame Seedless grapevines, grown on the experimental vineyard of Research Station Agriculture, EL-Matana, Esna, Luxor, Egypt to study the effect of organic and bio-fertilization on vegetative growth, nutrient status, and fruiting of Flame Seedless grapevines. The experiment was arranged in a complete randomized block design with seven treatments and three replications two vine per each. The obtained results could be summarized as follow: Using the recommended dose of nitrogen (RDN) via 50 or 25% mineral plus 50 or 75% or 50% organic and bio-form significantly increased, pruning wood weight, number of leaves/shoot and leaf area as well as leaf total chlorophyll and leaf nutrient composition compared to use RDN via mineral N fertilizer alone. No significant differences on these traits due to use RDN via 50% mineral plus 50 organic or bio, double form or 25% N plus 75 bio-form and triple born. All combined fertilization treatments significantly increased the yield and improved the cluster and berry traits compared to use RDN via mineral source only. It is evident from the foregoing results that double form 50% mineral plus 50% either organic or bio or triple form (25% N plus, 75 organic and bio.) improved the vegetative growth, yield and berry quality. In addition, it minimized the production costs and reduced environmental pollution.

Vineyards of ‘Niagara Rosada’ have shown great productive potential. However, the grape production has been affected by factors related to climate adversities. Thus, this study aimed to evaluate the effect of plastic cover and bagging of bunches of ‘Niagara Rosada’, in Almirante Tamandaré, PR, Brazil. Plastic cover used was of polyethylene with 250 µm of thickness and bagging of the bunches with white non-woven fabric bags. The treatments were: without plastic cover over the vineyard and without bagging of bunches (control); with plastic cover over the vineyard and without bagging of bunches; with plastic cover over the vineyard and with bagging of bunches; and without plastic cover over the vineyard and with bagging of bunches. To determine the quality of the grapes, we evaluated weight of the bunches and berry, width and length of the bunch, number of berries per bunch, total soluble solids (SS), titratable acidity (TA), pH, and SS/TA ratio. In both crop seasons, treatments with the protected plants or bunches were superior in all evaluated traits. The plastic cover and bagging of bunches delayed maturation and improved the physical and chemical characteristics of bunches and berries of ‘Niagara Rosada’. Os vinhedos de ‘Niagara Rosada’ têm mostrado grande potencial produtivo. No entanto, a produção da uva tem sido afetada por fatores relacionados a adversidades climáticas. Assim, este trabalho teve como objetivo avaliar o efeito da cobertura plástica e do ensacamento dos cachos de ‘Niagara Rosada’ em Almirante Tamandaré, PR, Brasil. A cobertura plástica utilizada foi de polietileno com espessura de 250 µm e o ensacamento dos cachos com tecido não texturizado (TNT) de cor branca. Os tratamentos foram: sem cobertura plástica do vinhedo e sem ensacamento dos cachos (Testemunha), com cobertura plástica do vinhedo e sem ensacamento dos cachos, com cobertura plástica do vinhedo e com ensacamento dos cachos e sem cobertura plástica do vinhedo e com ensacamento dos cachos. Para determinação da qualidade das uvas, avaliou-se massa dos cachos e bagas, largura e comprimento do cacho, número de bagas por cacho, teor de sólidos solúveis (SS), acidez titulável (AT), pH e relação SS/AT. Em ambas as safras, os tratamentos com proteção das plantas ou cachos apresentaram superioridade em todas as características avaliadas. A cobertura plástica e o ensacamento dos cachos atrasaram a maturação e melhoraram as características físicas e químicas dos cachos e bagas da uva ‘Niagara Rosada’.

The present study is focused on the determination of mineral composition of international red wines (Merlot, Cabernet Sauvignon), white wines (Sauvignon blanc, Aligoté, Muscat Ottonel, Italian Riesling) and native red wines (Feteasca neagră, Burgund mare, Băbeasca neagră), white wines (Feteasca regală, Fetească albă, Băbeasca gri, Șarba) produced in the Dealu Bujorului vineyard between 2014-2018, using Inductively Coupled Plasma Mass-Spectrometry (ICP-MS), and comparison of mineral composition of the soil. The high level of Ca, K, Na, Mg, P and Fe was observed in analyzed wine samples, the concentration of Na, Cu, As, Cd, Zn and Pb metals in analyzed wine samples were under Maximal Permissible Limit. The average concentrations of elements in the soil decrease in the following order: K, Na, Ca, Cu, Fe, P, Mg, Li, Co, Mn, Zn, Pb, Cr, As, U, Cd and Hg. Cu concentration in the topsoil of the Dealu Bujorului vineyard exceeds the maximum allowed limit (20 mg/kg Cu).

This study was conducted during the two consecutive seasons of (2016 and 2017) in a private vineyard located at El-Sadat City, Menoufia Governorate, to study the effect of gibberellic acid (GA3) and forchlorfnuron (CPPU) application on productivity and berries development of Early Sweet grape cultivar. The vines were five years old, spaced at 2×3 meters apart, grown in a sandy soil and trellised according to the Spanish Parron system. Eight treatments were applied by using dipping clusters with CPPU at concentrations from 1 to 7ppm after fruit set directly in combined plus spraying with GA3 at 20 ppm at 6-8mm berry diameter in additionally control treatment. The results revealed that all CPPU and GA3 applications had the best results in comparison with control in both seasons. Excessive CPPU doses + spraying clusters with GA3 increases yield and physical properties of berries i.e. berry weight, size and firmness, but it delay maturation. In contrast, lower CPPU doses + spraying clusters with GA3 cause a slightly in retarding maturation but reduces yield and its components. On the other hand, moderate CPPU doses (dipping clusters with CPPU at 5ppm + spraying clusters with GA3 at 20 ppm) had an achieving in appropriate balance between yield and cluster quality attributes of Early Sweet grapevines in both seasons.

The objective of the work was to compare different timing of leaf removal management and their effect on the chemical and physical characteristics of the Chardonnay grapevine in a high-altitude region of Santa Catarina State. This study was carried out in the 2018 and 2019 vintages, in a commercial vineyard, located in the municipality of Sao Joaquim - Santa Catarina State. Vineyard of the Chardonnay grapevine grafted on ‘Paulsen 1103’ was used. The treatments consisted of leaf removal, exposing the clusters region in five different phenological stages: full bloom, buckshot berries, pea-sized berries, veraison and plants without leaf removal (control). Productive indexes, bunch architecture and technological maturation were evaluated. The variables were subjected to analysis of variance (ANOVA) and when treatment effects were detected, the test of comparison of means was carried out using the Scott Knott Test at 5% probability of error. The leaf removal management of the 'Chardonnay' grapevine did not influence variables related to the cluster architecture (cluster mass, cluster length and number of berries), however, when carried out after the phenological stage buckshot berries provides an increase in the compaction index. The results obtained in the present work demonstrate that the defoliation management increases the productive indexes (production per plant and productivity) and improves the technological maturation of the grapevine (soluble solids), being recommended its realization until the phenological stage veraison.

Starmerella bacillaris is a non-Saccharomyces yeast recently proposed for grape fermentation in association with Saccharomyces cerevisiae. Due to its high glycerol and moderate volatile acidity production this yeast can contribute to improving wine quality. Some strains have been demonstrated to exhibit antifungal activity against grey mould on grape, which is caused by Botrytis cinerea. The simultaneous presence of these traits in S. bacillaris is of great interest. Indeed, this yeast can be potentially used as a biocontrol agent in vineyards. Research on the ability of S. bacillaris to survive or, even to grow on the surface of grapes is a starting point in the evaluatation of its potential use in vineyards. The preliminary results of our study showed that when applied to the grape surface under laboratory condictions, inoculum sized S. bacillaris with antifungal activity developed and lasted for at least 6 days in high concentrations. In addition, it positively influenced the fermentation process by producing high concentrations of glycerol (average value 4.89 ± 0.47 g/L). Interestingly, a positive effect on wine quality was also observed when the inoculum size was 10 times higher or lower than the reference concentration. When sprayed on the vines in the vineyard and present on the grape skin surface after harvest, S. bacillaris cells can start alcoholic fermentation.

The study aimed to identify the filamentous fungi and yeast mycobiota found on the surface and in grapes, grape must, and wine obtained from four red grape varieties: Alibernet, Dornfelder, Blue Frankish, Cabernet Sauvignon, and four white grape varieties: Green Veltliner, Rheinriesling, Pinot Blanc, Sauvignon. Grapes from vineyard Vrbové located in southwestern Slovakia were used for the research in 2020. The identification of filamentous fungi was performed using the macroscopic and microscopic observations and yeasts were identified by MALDI-TOF Mass Spectrometer. A total of 642 isolates were obtained. Grapes were rich in diversity of filamentous fungi (13 genera) and must on yeasts (8 genera). Penicillium, Botrytis, and Hanseniaspora uvarum were identified in both grapes and must. Three of the fungal genera identified by conventional or molecular techniques from the surface of red grape varieties were predominant: Alternaria (26%), Botrytis (21%), and Issatchenkia terricola (13%), two from endogenous mycobiota: Hanseniaspora uvarum (45%) and Botrytis (17%), four from the surface of white grape varieties: Penicillium (25%), Botrytis (21%), Alternaria (16%) and Hanseniaspora uvarum (15%), and three from endogenous mycobiota: Botrytis (44%), Hanseniaspora uvarum (23%) and Alternaria (20%). Saccharomyces cerevisiae, Candida krusei, C. utilis, and Cryptococcus neoformans were identified only in wine.

The present investigation was carried out during winter of the consecutive seasons (2018/2019 and 2019/2020) on 9 year old Flame Seedless grapevines grown in a private vineyard at Sharkia Governorate, Egypt. Garlic (Balady cv) was intercropped with grapevines (Flame Seedless cv) on dripper irrigation lines, as a try to reduce numbers of phytonematodes in grapevine soil. For grapevines, the obtained results referred that intercropping insignificantly (P≤0.05) increased number of berries/bunch, weight of 100 berries and berry firmness, separation force, total soluble solids percentage, anthocyanin content and yield/ vine as well as bunch weight and width, berry length, berry diameter and berry shape index compared to sole grapevines. For garlic (Balady cv), the achieved results pointed out that plant height, number of leaves/plant, leaf fresh weight/ plant, nick diameter as well as bulb diameter, height and weight/ plant insignificantly decreased when garlic intercropped with grapevines compared to control. Competition indices; i.e., land equivalent ratio (LER), area time equivalent ratio (ATER) and land utilization efficiency (LUE) showed that, intercropping garlic with grapevines was more efficient than sole cropping system. Aggressively estimation demonstrated that grapevines were dominant advantages while garlic was dominated. Moreover, intercropping garlic (Balady cv) with grapevines was obviously decreased population density of phytonematodes infesting grapevine orchard. Generally, garlic together with other biocontrol agents in grapevine orchards has the potential to be an efficient and environmentally agricultural management method to reduce numbers of plant parasitic nematodes.

Berry color development of Flame Seedless grapes is considered one of the main maturity indices. Achieved the requested color at harvest is one of the main problems of Flame Seedless because berries fail to reach the requested level of red color. So, an experiment was carried out on Flame Seedless vines in a private vineyard in the governorate of Sharkia, Egypt. Bunches were sprayed with one of the following five treatments, 0 mg/l (as a control treatment), Methyle Jasmonate at 0.046 %, Ethephon at 0.24 g/l and at 0.14 g/l, Ethephon at 0.24 g/l + ABA at 0.015% and Ethephon at 0.24 g/l + Methyle Jasmonate at 0.046 %. The single treatments were sprayed at veraison stage while the combined treatments were sprayed at veraison stage and then 5 days later. The combined treatment of (Ethephon + Methyle Jasmonate) was the best treatment, compared control treatment in both seasons, it improves berry color and increases the packable yield at the first harvest. The same treatment significantly increased anthocyanin content and total soluble solids percentage as well as TSS/acid ratio compared with control treatment in both seasons, respectively. Therefore, the combination between Ethephon and Methyle Jasmonate surpassed the other tested treatments in improving berry color and improving berry quality at harvest time. Moreover, the combined treatments with either Ethephon + Methyle Jasmonate was more effective in improving berry coloration and quality than Methyle Jasmonate individually.

The effect of different concentrations of aminoethoxyvinylglycine (AVG; 0 (control), 250, 500, 750 and 1000 mg/l), an ethylene biosynthesis inhibitor, on grape berry set and fruit quality was evaluated under vineyard conditions. Treatments were performed on ‘Alphonse Lavallée’ grape cultivar at full bloom stage for two years. Cluster and berry growth (length, width and weight), firmness, soluble solids content, yield, berry set, anthocyanin content and colour parameters were determined on treated and untreated (control) fruits. As a result of the study, the effects of AVG concentrations on firmness were not significant. When AVG applications were compared with control, it is determined that some quality properties were statistically affected, the others were not. Fruit set was increased by lower concentrations of AVG (250 mg/l) and anthocyanin content was increased by higher concentrations of AVG (750 mg/l).

This study was conducted during 2018, 2019 and 2020 seasons, on 10 years old Flame Seedless grapevines, grown on the experimental vineyard of the Research Station, Matana, Esna, Luxor, Egypt to study the effect of antioxidants on vegetative growth, nutrient status, and fruiting of Flame Seedless grapevine. The experiment was arranged in a complete randomized block design with four treatments and three replications two vine per each. The obtained results could be summarized as follow: Spraying either citric acid, ascorbic acid or vitamin B12 significantly increased, pruning wood weight and leaf area as well as leaf total chlorophyll and leaf nutrient composition compared to spray water ones (check treatment). No significant differences on these traits due to spray with any ones compared to either. All antioxidants treatments significantly increased the yield and improved the cluster and berry traits compared to sprayed water. It is evident from the foregoing results that spraying with either ascorbic acid or citric acid or vitamin B12 at 250 ppm three times improved the vegetative growth, yield and berry quality.

Wine grape berries (Vitis spp.) harbor a wide variety of yeasts and filamentous fungi that impact grapevine health and the winemaking process. Identification of these fungi could be important for controlling and improving wine production. The use of high-throughput sequencing (HTS) strategies has enabled identification and quantification of bacterial and fungal species in vineyards. The aims of this study were to identify mycobiota from Cabernet Sauvignon and Zinfandel (V. vinifera), Carlos and Noble muscadines (V. rotundifolia), Cynthiana (V. aestivalis), and Vignoles hybrid (cross of different Vitis spp.) grapes, and investigate the effect of grape variety, location, and year on grape fungal communities. Grape berries were collected in 2016 and 2017 from four vineyards located in Arkansas. The HTS of the Internal Transcribed Spacer 1 region was used to identify grape indigenous epiphytic and endophytic fungal communities. The predominant genera identified on the Arkansas wine grapes were Uwebraunia, Zymoseptoria, Papiliotrema, Meyerozyma, Filobasidium, and Curvibasidium. Overall, the data suggested that grape fungal community distribution and relative abundance were influenced by grape variety, year, and location, but each was influenced to a different extent. Not only were grape mycobiota influenced by year, variety, and location but also it appeared that communities from the previous year impacted microbial communities the following year. For example, an increase of the mycoparasite Ampelomyces quisqualis was noticed in 2017 on grapes that carried the causal agent of powdery mildew, Erysiphe necator, in 2016, thus, amplifying the importance of vineyard microbiota knowledge for disease management and winemaking.

Experience in studying the rootability of grape cuttings was conducted on the territory of the vineyard of the Precaspian agrarian federal scientific center of the Russian academy of sciences, located in the Chernoyarsky district of the Astrakhan region. The research period is from 2015 to 2019. The relevance of the research is due to the insufficient availability of local planting material in the regions of the Russian Federation, as well as the high efficiency of the method for obtaining grape seedlings on their own roots. The purpose of the research was to study the survival rate of grape cuttings in the crop and identify varieties that are characterized by the best rooting ability. The article presents data on the ability to root cuttings of grapes of 18 table varieties of different maturation periods. The lowest rooting rate in the experiment had varieties Cardinal, Straseni, Memory Negros, the Gift to Zaporozhye, Karaburnu. It was found that the use of early – maturing varieties Kodryanka, Bogatyrsky, Lora, middle – matured-Husain pink and Lowland, late-matured-Moscow, Sevan, Soviet, the level of rootability of which varied from 44.2 to 71.4 %, is optimal for obtaining root-related seedlings of grapes.These varieties have a good ability to rhizogenesis, which allows us to recommend them for accelerated propagation of grape varieties.

Pruned vine-shoots used as wine additives have proven to be a useful tool for improving and differentiating wines. This is because they accumulate substances from the plant itself, which later, as a result of the toasting process, express their greatest oenological potential. However, vine being one of the crops subject to the most phytosanitary treatments, including fungicides in particular, it can be assumed that their residues will accumulate in the vine-shoots and pass into the wine. The aim of this study was to determine the content in pruned vine-shoots of four of the main fungicides applied in the vineyard in Spain: trifloxystrobin, boscalid, kresoxim-methyl and penconazole. In order to do so, a HPLC-MS/MS method was developed, which showed high reliability given its adequate validation parameters. The method was linear for the concentration range studied, LOD was 0.003 mg/kg and LOQ was 0.01 mg/kg, which comply with the control criteria.The evolution of residues in vine-shoots complying with critical agricultural practices and good agricultural practices was monitored at 1, 3 and 6 months of storage after pruning and, in the latter case, after being subjected to a toasting process. The dissipation of all the residues was demonstrated to be affected by storage duration and toasting. It was confirmed that converting vine-shoots into oenological additives for wines will not pose a risk to the consumer if good agricultural practices are applied.

‘Microbial terroir’ relates to the influence of autochthonous yeasts associated with a grape cultivar on the resultant wine. Geographic region, vineyard site and topography, climate and vintage influence the biodiversity of these microbial communities. Current research focus attempts to correlate their ‘microbial fingerprint’ to the sensorial and chemical characteristics of varietal wines from distinct geographical wine regions. This study focuses on the minor red grape variety, Negro Saurí, which has seen a resurgence in the León Appellation of Origin in Spain as a varietal wine. An experimental vineyard at Melgarajo S.A. (42° 15′ 48.68_N 5° 9′ 56.66_W) was sampled over four consecutive vintages, with autochthonous yeasts being isolated from grapes, must and pilot-scale un-inoculated fermentations, and identified by ITS sequencing. Forty-nine isolates belonging toMetschnikowia pulcherrima,Lachancea thermotolerans,Hanseniaspora uvarumandTorulaspora delbrueckiiwere isolated from grapes and must, and early stages of fermentation dependent on seasonal variation.Saccharomyces cerevisiaepredominated throughout fermentation, as a heterogeneous and dynamic population, with seven major biotypes identified amongst 110 isolates across four consecutive vintages. Twenty-fourS. cerevisiaeisolates representing five strains dominated in two or more vintages. Their persistence through fermentation warrants further validation of their oenological properties as starter cultures.

Alternative materials with added-value functions, such as phytopathogen suppression and biostimulant and/or biofertilising activity, have been proposed as peat substitutes in growing media. The aim of this work was to evaluate the effect of 23 agro-industrial composts as components of growing media for baby-leaf lettuce transplant production and their activity against the plant pathogen Pythium irregulare. The composts were produced by mixing different starting feedstocks—tomato waste, leek waste, olive mill cake and vineyard pruning waste—with different additives (coffee, thyme, lavender and rockrose waste), which were incorporated at the beginning of the maturation phase. The results obtained indicated that the composts were mature enough to be used as growing media. The fresh weight of the lettuce plants grown with the different composts was significantly higher than in plants obtained with peat. Composts with the coffee additive produced higher lettuce fresh weight, while those with thyme yielded a lower fresh weight. Moreover, composts as components of growing media showed significantly higher P. irregulare suppressiveness than peat. The composts with additives produced lower lettuce fresh weight than composts without additives, but showed higher suppressiveness. Composts with additives showed opposite results depending on whether they were exposed to pathogens or not. Composts with additives showed opposite results according to pathogen pressure or not. Out of all the composts studied, the compost with tomato waste and leek waste as the initial feedstock, and lavender as an additive, showed the highest suppressive capacity. After lettuce harvesting, the growing media with composts showed significantly lower concentrations of P. irregulare than peat. Principal Component Analysis (PCA) revealed that the growing media with compost can be grouped together according to the additive type, This work has been supported by the Spanish Ministry of Economy and Competitiveness and by European Regional Development Funds (ERDF, ‘‘Una manera de hacer Europa”) in the framework of the project ‘‘Compost as biofertilizer, resistance inductor against plant pathogens and healthy property promoter under a crop intensive sustainable production. Compoleaf” (Project AGL2017- 84085-C3-1-R, C3-2-R and C3-3-R)

With the recent advancement of next-generation sequencing methods, there has been an increase in studies on identification of vineyard microbiota, winery-associated microbiota, and microbiota in wine fermentation. However, there have been few studies investigating the fungal microbiota of table grapes which present distinct spoilage and food safety challenges. The aims of this study were to identify and compare the impact of year, variety, and vineyard location on grape, leaf, and soil fungal communities of two varieties of table grapes, Faith and Gratitude, grown in two open-air vineyards and one high tunnel vineyard. The grape, leaf, and soil mycobiota were analyzed using high throughput amplicon sequencing of the ITS region. The sampling year and location of table grapes had an impact on grape, leaf, and soil mycobiota. Fungal diversity of grape, leaf, and soil was greater in 2017 than in 2016. Grape and leaf samples presented strong similarities in fungal communities with abundance of Sporidiobolaceae and Filobasidium in two vineyards and Cladosporium in another one. The high tunnel structure had distinct grape and leaf fungal communities compared to the two other vineyard locations. Mortierella was the predominant genus (27%) in soil samples for the three locations; however, genera of lower abundance varied between locations. These results provide extensive description of fungal communities in less-studied table grape vineyards and high tunnels, providing useful insight of potential threats and preventive strategies to help improve the production and marketability of table grapes.

Grapevine leafroll-associated virus 3 (GLRaV-3) is a worldwide pathogen of Vitis negatively affecting wine production. In red berry cultivars grown in New Zealand, the foliar changes to GLRaV-3-infected vines inform decisions on vine removal (roguing). However, roguing does not always contain GLRaV-3 spread in the presence of an insect vector like the mealybug, Pseudococcus calceolariae. Virus incidence and mealybug abundance data collected annually over 7 years were modelled under differing scenarios to ascertain support for roguing. In a simulated 1 ha vineyard planted in 2500 mature Merlot vines, simulations over 20 years evaluated the effectiveness of roguing, ‘rogue 1 + 2’ (concurrent roguing of symptomatic plus both within-row neighbouring vines), inefficient roguing (50% of symptomatic vines rogued), and ‘no-action’. The model used variable initial GLRaV-3 incidence (0.4, 5, 10, 15 and 20%), and low, median and high vector densities (6, 26 and 75 mealybugs per 100 vine leaves, respectively). Roguing was the optimal response to GLRaV-3, independent of the initial incidence, but results were vector density dependent. At a low vector density, roguing relative to the other responses tested, sustained the lowest annual GLRaV-3 incidence, the least need to plant replacement vines and the lowest estimated average annual costs plus loss of income. At median and high vector densities, roguing remained the most favourable response but virus control was less effective and the costs incurred were higher. Thus, for vineyards affected by GLRaV-3, achieving economic sustainability relies on integrating efficient roguing with effective vector management.

Grape powdery mildew caused byErysiphe necatoris a limiting factor in grape production. In order to develop effective management strategies, the factors influencing the survival of the fungus need to be identified and characterized accordingly. For this purpose, we focused on the effect of weather conditions during overwintering on the survival and viability of ascospores. In spring 2017 and 2018, grape leaf litter and bark samples were collected and examined to determine the density of chasmothecia and the viability of ascospores in various Austrian vine growing regions. There were obvious differences in the amounts of chasmothecia between both years and all examined vineyards. Lower quantities of chasmothecia were detected on the exfoliating bark compared to leaf litter, with up to 37% of chasmothecia containing viable ascospores. In comparison, chasmothecia from leaf litter showed a lower viability (up to 5%). The number of viable ascospores per head of vine ranged from 0 to 351 and from 0 to 251 in 2017 and 2018, respectively, and showed partly a strong variation within one location in both years. The infectivity of ascospores on detached leaves was confirmed. In a survival experiment, chasmothecia, when incubated at 7 °C, released more viable ascospores than chasmothecia incubated at 17 °C. After an incubation period of 30 weeks, only chasmothecia stored at the lower temperature contained viable ascospores. However, the mean temperature differences of 0.1 to 1.2 °C during the period of formation of chasmothecia to bud break in both years and six investigated areas did not explain differences in the viability of the ascospores. Differences in vineyard management seem to be of particular importance here.