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Low-cost smartphone-compatible portable ophthalmoscopes can capture visuals of the patient's retina to screen several ophthalmological diseases like glaucoma. The images captured have lower quality and resolution than standard retinography devices but enough for glaucoma screening. Small videos are captured to improve the chance of inspecting the eye properly; however, those videos may not always have enough quality for screening glaucoma, and the patient needs to repeat the inspection later. In this paper, a method for automatic assessment of the quality of videos captured using the D-Eye lens is proposed and evaluated with a personal dataset with 539 videos. Based on two methods developed for retina localization on the images/frames, the Circle Hough Transform method with a precision of 78,12% and the YOLOv7 method with a precision of 99,78%, the quality assessment method automatically decides on the quality of the video by measuring the number of frames of good-quality in each video, according to the chosen threshold. [ABSTRACT FROM AUTHOR]