8 results on '"D'Agostino, Donna M."'
Search Results
2. Functional properties and sequence variation of HTLV-1 p13.
- Author
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Omsland, Maria, Silic-Benussi, Micol, Moles, Ramona, Sarkis, Sarkis, Purcell, Damian F. J., Yurick, David, Khoury, Georges, D'Agostino, Donna M., Ciminale, Vincenzo, and Franchini, Genoveffa
- Subjects
HTLV ,MOLECULAR cloning - Abstract
Human T cell leukemia virus type-1 (HTLV-1) was the first retrovirus found to cause cancer in humans, but the mechanisms that drive the development of leukemia and other diseases associated with HTLV-1 infection remain to be fully understood. This review describes the functional properties of p13, an 87-amino acid protein coded by HTLV-1 open reading frame II (orf-II). p13 is mainly localized in the inner membrane of the mitochondria, where it induces potassium (K
+ ) influx and reactive oxygen species (ROS) production, which can trigger either proliferation or apoptosis, depending on the ROS setpoint of the cell. Recent evidence indicates that p13 may influence the cell's innate immune response to viral infection and the infected cell phenotype. Association of the HTLV-1 transcriptional activator, Tax, with p13 increases p13's stability, leads to its partial co-localization with Tax in nuclear speckles, and reduces the ability of Tax to interact with the transcription cofactor CBP/p300. Comparison of p13 sequences isolated from HTLV-1-infected individuals revealed a small number of amino acid variations in the domains controlling the subcellular localization of the protein. Disruptive mutations of p13 were found in samples obtained from asymptomatic patients with low proviral load. p13 sequences of HTLV-1 subtype C isolates from indigenous Australian patients showed a high degree of identity among each other, with all samples containing a pattern of 5 amino acids that distinguished them from other subtypes. Further characterization of p13's functional properties and sequence variants may lead to a deeper understanding of the impact of p13 as a contributor to the clinical manifestations of HTLV-1 infection. [ABSTRACT FROM AUTHOR]- Published
- 2020
- Full Text
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3. Effects of human T-cell leukemia virus type 1 (HTLV-1) p13 on mitochondrial K+ permeability: A new member of the viroporin family?
- Author
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Silic-Benussi, Micol, Marin, Oriano, Biasiotto, Roberta, D’Agostino, Donna M., and Ciminale, Vincenzo
- Subjects
HTLV ,PERMEABILITY ,OXYGEN in the body ,APOPTOSIS ,MEMBRANE proteins ,MITOCHONDRIA ,POTASSIUM channels ,HOMEOSTASIS - Abstract
Abstract: Human T-cell leukemia virus type-1 (HTLV-1) encodes a mitochondrial protein named p13. p13 mediates an inward K
+ current in isolated mitochondria that leads to mitochondrial swelling, depolarization, increased respiratory chain activity and reactive oxygen species (ROS) production. These effects trigger the opening of the permeability transition pore and are dependent on the presence of K+ and on the amphipathic alpha helical domain of p13. In the context of cells, p13 acts as a sensitizer to selected apoptotic stimuli. Although it is not known whether p13 influences the activity of endogenous K+ channels or forms a channel itself, it shares some structural and functional analogies with viroporins, a class of small integral membrane proteins that form pores and alter membrane permeability. [Copyright &y& Elsevier]- Published
- 2010
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4. Mitochondrial targeting of the p13II protein coded by the x-II ORF of human T-cell leukemia/lymphotropic virus type I (HTLV-I).
- Author
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Ciminale, Vincenzo, Zotti, Lorenza, D'Agostino, Donna M, Ferro, Tiziana, Casareto, Luca, Franchini, Genoveffa, Bernardi, Paolo, and Chieco-Bianchi, Luigi
- Subjects
HTLV ,GENOMES ,IMMUNOFLUORESCENCE ,PROTEINS ,MITOCHONDRIA - Abstract
The X region of the HTLV-I genome contains four major open reading frames (ORFs), two of which, termed x-I and x-II, are of still undefined biological significance. By indirect immunofluorescence and dual labeling with marker proteins, we demonstrate that p13
II , an 87-amino acid protein coded by the x-II ORF, is selectively targeted to mitochondria. Mutational analysis revealed that mitochondrial targeting of p13II is directed by an atypical 10-amino acid signal sequence that is not cleaved upon import and is able to target the Green Fluorescent Protein to mitochondria. Expression of p13II results in specific alterations of mitochondrial morphology and distribution from a typical string-like, dispersed network to round-shaped clusters, suggesting that p13II might interfere with processes relying on an intact mitochondrial architecture. Functional studies of mitochondria with the cationic fluorochrome tetramethylrhodamine revealed that a subpopulation of the cells with p13II -positive mitochondria show a disruption in the mitochondrial inner membrane potential (Δψ), an early event observed in cells committed to apoptosis. Taken together, these results suggest novel virus-cell interactions that might be important in HTLV-I replication and/or pathogenicity. [ABSTRACT FROM AUTHOR]- Published
- 1999
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5. Coding potential of the X region of human T-cell leukemia/lymphotropic virus type II.
- Author
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Ciminale, Vincenzo and D'Agostino, Donna M.
- Subjects
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HTLV , *GENE expression - Abstract
Discusses the expression strategies leading to the increased coding potential of the X region of the complex human retrovirus HTLV-II. Possible basis for pathogenic differences between HTLV-II and HTLV-I; Alternative splicing and translation of more than one protein of the same mRNA; Complex life cycle and pathogenicity of HTLV-I and HTLV-II.
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- 1996
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6. Control of ROS production and T-cell turnover by HTLV-p13.
- Author
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Silic-Benussi, Micol, Cavallari, Ilaria, Chieco-Bianchi, Luigi, di Lisa, Fabio, D'Agostino, Donna M., Bernardi, Paolo, and Ciminale, Vincenzo
- Subjects
HTLV ,REACTIVE oxygen species ,T cells - Abstract
An abstract of the research paper titled,"Control of reactive oxygen species (ROS) production and T-cell turnover by the Human T-cell leukemia virus type 13 (HTLV-p13)," is presented.
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- 2011
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7. Functional Domain Structure of Human T-Cell Leukemia Virus Type 2 Rex.
- Author
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Narayan, Murli, Younis, Ihab, D'Agostino, Donna M., and Green, Patrick L.
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HTLV , *VIRAL proteins , *VIRAL genetics - Abstract
The Rex protein of human T-cell leukemia virus (HTLV) acts posttranscriptionally to induce the cytoplasmic expression of the unspliced and incompletely spliced viral RNAs encoding the viral structural and enzymatic proteins and is therefore essential for efficient viral replication. Rex function requires nuclear import, RNA binding, multimerization, and nuclear export. In addition, it has been demonstrated that the phosphorylation status of HTLV-2 Rex (Rex-2) correlates with RNA binding and inhibition of splicing in vitro. Recent mutational analyses of Rex-2 revealed that the phosphorylation of serine residues 151 and 153 within a novel carboxy-terminal domain is critical for function in vivo. To further define the functional domain structure of Rex-2, we evaluated a panel of Rex-2 mutants for subcellular localization, RNA binding capacity, multimerization and trans-dominant properties, and the ability to shuttle between the nucleus and the cytoplasm. Rex-2 mutant S151A,S153A, which is defective in phosphorylation and function, showed diffuse cytoplasmic staining, whereas mutant S151D,S153D, previously shown to be functional and in a conformation corresponding to constitutive phosphorylation, displayed increased intense speckled staining in the nucleoli. In vivo RNA binding analyses indicated that mutant S151A, SI53A failed to efficiently bind target RNA, while its phosphomimetic counterpart, S151D,S153D, bound twofold more RNA than wild-type Rex-2. Taken together, these findings provide direct evidence that the phosphorylation status of Rex-2 is linked to cellular trafficking and RNA binding capacity. Mutants with substitutions in either of the two putative multimerization domains or in the putative activation domain-nuclear export signal displayed a dominant negative phenotype as well as defects in multimerization and nucleocytoplasmic shuttling. Several carboxy-terminal mutants that displayed wild-type levels of phosphorylation and localized to the nucleolus were also partially impaired in shuttling. This is consistent with the hypothesis that the carboxy terminus of Rex-2 contains a novel domain that is required for efficient shuttling. This work thus provides a more detailed functional domain map of Rex-2 and further insight into its regulation of HTLV replication. [ABSTRACT FROM AUTHOR]
- Published
- 2003
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8. Modulation of mitochondrial K+ permeability and reactive oxygen species production by the p13 protein of human T-cell leukemia virus type 1
- Author
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Silic-Benussi, Micol, Cannizzaro, Enrica, Venerando, Andrea, Cavallari, Ilaria, Petronilli, Valeria, La Rocca, Nicoletta, Marin, Oriano, Chieco-Bianchi, Luigi, Di Lisa, Fabio, D'Agostino, Donna M., Bernardi, Paolo, and Ciminale, Vincenzo
- Subjects
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VIRAL proteins , *HTLV , *PERMEABILITY , *GENE expression , *REACTIVE oxygen species , *POTASSIUM channels , *HYDROGEN-ion concentration , *BIOLOGICAL assay - Abstract
Abstract: Human T-cell leukemia virus type-1 (HTLV-1) expresses an 87-amino acid protein named p13 that is targeted to the inner mitochondrial membrane. Previous studies showed that a synthetic peptide spanning an alpha helical domain of p13 alters mitochondrial membrane permeability to cations, resulting in swelling. The present study examined the effects of full-length p13 on isolated, energized mitochondria. Results demonstrated that p13 triggers an inward K+ current that leads to mitochondrial swelling and confers a crescent-like morphology distinct from that caused by opening of the permeability transition pore. p13 also induces depolarization, with a matching increase in respiratory chain activity, and augments production of reactive oxygen species (ROS). These effects require an intact alpha helical domain and strictly depend on the presence of K+ in the assay medium. The effects of p13 on ROS are mimicked by the K+ ionophore valinomycin, while the protonophore FCCP decreases ROS, indicating that depolarization induced by K+ vs. H+ currents has different effects on mitochondrial ROS production, possibly because of their opposite effects on matrix pH (alkalinization and acidification, respectively). The downstream consequences of p13-induced mitochondrial K+ permeability are likely to have an important influence on the redox state and turnover of HTLV-1-infected cells. [Copyright &y& Elsevier]
- Published
- 2009
- Full Text
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