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Background: The aim is to analyse physical activity (PA), the fulfilment recommendation of at least 150 min of moderate PA, through walking/biking (W&B), sport, both types of PA and the factors associated with inactivity by Spanish women who attended breast cancer screening programmes. Methods: The DDM-Spain is a multicentre cross-sectional study involving 3584 women, aged 45-68, attending screening in seven Spanish cities. Data were collected using a questionnaire, including age, socio-demographic and lifestyle characteristics, family burden and PA. PA was converted into metabolic equivalent of task (METs), categorized as low = 3000 METs m/w. A multivariate logistic regression was performed to identify variables associated with inactivity for each type of PA. Results: No women achieved a high level of PA through sport. 79.2% achieved a high or moderate level of PA by W&B. Lack of sport was associated with being overweight (odds ratio OR= 1.31; 95% confidence interval CI: 1.06 to 1.62), body mass index (BMI) >= 30 (OR = 1.85; 95% CI: 1.44 to 2.38), smoking (OR = 1.56; 95% CI: 1.22 to 2.00) and living with a disabled person (OR = 1.64; 95% CI: 1.0 to 2.81), whereas enough sport practice was associated with higher educational or socio-economic level (SEL). Regarding W&B, inactivity was associated with BMI >= 30 (OR = 1.91; 95% CI: 1.49 to 2.45) and living with someone > 74 (OR = 1.96; 95% CI: 1.48 to 2.58). Inactivity for both types of exercise was associated with a BMI >= 30 (OR = 2.13; 95% CI: 1.63 to 2.8), smoking (OR = 1.41; 95% CI: 1.09 to 1.81) and living with someone > 74 (OR = 1.69; 95% CI: 1.24 to 2.28). Conclusions: Family burden and BMI >= 30 are inversely associated with both types of PA. W&B is the most common type of PA regardless of educational and SEL.