1. Expression of mucin-2 and correlation with PAS-alcian blue stain in Barrett's esophagus
- Author
-
César V, Lopes, Júlio, Pereira-Lima, and Antônio, Hartmann
- Subjects
Adult ,Aged, 80 and over ,Male ,Mucin-2 ,Staining and Labeling ,Mucins ,Middle Aged ,Periodic Acid-Schiff Reaction ,Immunohistochemistry ,Barrett Esophagus ,Humans ,Female ,Alcian Blue ,Coloring Agents ,Aged - Abstract
Hematoxylin-eosin stain may fail to show intestinal metaplasia in Barrett's esophagus, and PAS-alcian blue may present difficulties of interpretation due to its more heterogeneous staining. We investigated whether Mucin-2 is a good substitute for PAS-alcian blue in the detection of goblet cells.Biopsy specimens from 47 Barrett's esophagus patients were stained with hematoxylineosin and PAS-alcian blue. Mucin-2 expression was evaluated with monoclonal antibody. The intra- and interobserver agreements about the expression of Mucin-2 and PAS-alcian blue stain were determined by kappa statistics.PAS-alcian blue and Mucin-2 were positive in all cases. Mucin expression was positive in goblet cells and few columnar cells. PAS-alcian blue showed heterogeneous staining. The columnar epithelium and the submucosal glands were also stained. Intra- and interobserver agreement in the identification of intestinal metaplasia was 100%.Mucin-2 agreed fully with PAS-alcian blue. The intra- and interobserver agreement was perfect, justifying its use in the diagnosis of Barrett's esophagus.
- Published
- 2006