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Meningitis continues to be a formidable illness with high morbidity and mortality among children in India. The present study was undertaken to prospectively look for the prevalence of pyogenic meningitis at Gulbarga and to find out the utility of gram stain, Latex Agglutination Test and (LTA) and C-reactive protein in the rapid diagnosis of pyogenic meningitis from children. Over a 48-months period, 535 children with a presumptive clinical diagnosis of acute bacterial meningitis were investigated by direct microscopy, CRP, bacterial culture, latex agglutination test (L TA), cell count and cell type and biochemical tests. Latex Agglutination Test (LA T) was done for detection of the antigens of Streptococcus pneumoniae, Group B Streptococci, E. coli, Neisseria meningitidis and Haemophilus influenzae type b. Among 535 suspected meningitis cases, 291 cases were diagnosed as pyogenic meningitis cases based on biochemical tests, cell count and cell type. Out of 291 cases, 55 cases have already received antibiotic treatment. Among 236 cases of untreated pyogenic meningitis cases, 199 CSF samples were culture positive. Streptococcus pneumoniae (44.7%) was the predominant organism identified, followed by H influenzae (25.6%) and Gp. B. Streptococci (9.5%). 208 of 236 cases were gram-stain positive, 129 cases had elevated CSF-CRP and 214 cases were diagnosed as pyogenic meningitis by the detection of bacterial antigens by latex agglutination test. Among 55 pretreated cases, only 05 (9.1%) CSF samples were culture positive, bacteria was observed in 36 gram stain smear, CRP was elevated in 16 CSF samples and 52 pretreated cases of suspected meningitis were diagnosed as pyogenic meningitis by latex agglutination test for detection of bacterial antigens. Many of the bacterial isolates were sensitive to gentamicin, cefotaxime and ceftriaxone and least sensitive to tetracycline and gentamicin. 13.1% of gram-negative bacilli were ESBL producers. To conclude, inclusion of latex agglutination test for detection of bacterial antigen in the routine diagnosis adds a valuable adjunct in the rapid and accurate diagnosis of pyogenic meningitis.

Septicemia is the leading cause of morbidity and mortality in neonates. In this study, 828 consecutive neonates suspected of having septicemia from various neonatal intensive care unit hospitals in Gulbarga, Karnataka were investigated for isolation of microorganisms. Two samples of blood were collected aseptically for isolating the etiology. The cultures were positive in 346 (41.9%) cases for aerobic bacteria and 68 (8.3%) for Candida species. Among Candida species, C. tropicalis--27(39.7%) was the predominant organism followed by C. albicans 16(23.5%) and C. guillermondi 13(19.1%). The standard macrobroth dilution method was carried out to determine the minimum inhibitory concentration (MIC); C. krusei ATCC 6258 standard strain was included for quality control purpose. 4(25.0%) stains of C. albicans were resistant to amphotericin-beta and 2(12.5%) to fluconazole. High-level resistance to fluconazole was observed in C. krusei 4(40.0%).