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Your search keyword '"E. V. Karamov"' showing total 3 results
Start Over Author "E. V. Karamov" Topic humans
3 results on '"E. V. Karamov"'

1. Extreme founder effect in an HIV type 1 subtype A epidemic among drug users in Svetlogorsk, Belarus

Author

V V, Lukashov, E V, Karamov, V F, Eremin, L P, Titov, and J, Goudsmit

Subjects
Adult, Adolescent, Republic of Belarus, Molecular Sequence Data, HIV Core Protein p24, Gene Products, gag, HIV Infections, Sequence Analysis, DNA, HIV Envelope Protein gp120, Founder Effect, Peptide Fragments, Disease Outbreaks, HIV-1, Humans, Amino Acid Sequence, Substance Abuse, Intravenous
Published
1998

2. [Culturing T-lymphoblastic cell lines in media containing growth proteins]

Author

G V, Kornilaeva, R Ia, Podcherniaeva, E I, Mel'nichenko, and E V, Karamov

Subjects
Swine, T-Lymphocytes, Animals, Humans, Cattle, Blood Proteins, Cell Division, Cell Line, Culture Media
Abstract

Two optimal variants of growth medium with different content of growth proteins obtained from cattle and porcine blood sera are recommended for culturing lymphoblastoid cells. Use of growth proteins helps optimize the conditions of cell culturing by ruling out the toxic effect and Mycoplasma contamination and ensures the standardization of virological experiments due to the absence of gamma-globulin fraction.

Published
1997

3. False-positive sera do not react with human immunodeficiency virus (HIV)Gag-encoded recombinant antigen

Author

E. V. Karamov, Michael Bukrinsky, E. V. Barsov, V. A. Syrtsev, S. A. Popov, and S. A. Chaplinskas

Subjects
Genes, Viral, HIV Antigens, viruses, Blotting, Western, HIV Core Protein p24, Retroviridae Proteins, Human immunodeficiency virus (HIV), Gene Products, gag, Enzyme-Linked Immunosorbent Assay, Biology, medicine.disease_cause, gag Gene Products, Human Immunodeficiency Virus, Epitope, Serology, law.invention, Epitopes, Viral Proteins, Antigen, Western blot, immune system diseases, law, Virology, Immunopathology, medicine, Humans, False Positive Reactions, medicine.diagnostic_test, Immune Sera, HIV, virus diseases, Recombinant Proteins, Infectious Diseases, Recombinant DNA, Viral disease
Abstract

Ten sera from healthy blood donors positive by enzyme-linked immunoadsorbent assay (ELISA) were studied by immunoblot assay using natural and recombinant proteins. They interacted only with p17 or p24 proteins but were nonreactive with a recombinant protein (RP 50), which carries antigenic determinants to p17 and p24. Reactions were not blocked by preincubation of sera with genetically engineered p17 and p24 or purified viral p24, indicating that some new epitopes were formed during the Western blot procedure. Recombinant gag-encoded protein is required for confirmation of human immunodeficiency virus (HIV) seropositivity.

Published
1989
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