1. Author Correction: The Kunitz Domain I of Hepatocyte Growth Factor Activator Inhibitor-2 Inhibits Matriptase Activity and Invasive Ability of Human Prostate Cancer Cells
- Author
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Hsin-Fang Tu, Chun-Jung Ko, Pei-Wen Hsiao, Hsiang-Po Huang, Chen-Hsin Teng, Tai-Shan Cheng, Hsin-Ying Lin, Ming-Shyue Lee, Hsin-Hsien Lin, Shao-Wei Lan, Chung-Hsin Chen, Shang-Ru Wu, and Ya-Ting Tu
- Subjects
Male ,Serine Proteinase Inhibitors ,lcsh:Medicine ,Human prostate ,Text mining ,Protein Domains ,Cell Movement ,Cell Line, Tumor ,Animals ,Humans ,Matriptase ,Amino Acid Sequence ,lcsh:Science ,Author Correction ,Mice, Inbred BALB C ,Membrane Glycoproteins ,Multidisciplinary ,Sequence Homology, Amino Acid ,biology ,business.industry ,lcsh:R ,Serine Endopeptidases ,Prostatic Neoplasms ,HEPATOCYTE GROWTH FACTOR ACTIVATOR INHIBITOR ,HEK293 Cells ,Proteolysis ,Cancer cell ,biology.protein ,Cancer research ,lcsh:Q ,RNA Interference ,Kunitz domain ,business - Abstract
Dysregulation of pericellular proteolysis is often required for tumor invasion and cancer progression. It has been shown that down-regulation of hepatocyte growth factor activator inhibitor-2 (HAI-2) results in activation of matriptase (a membrane-anchored serine protease), human prostate cancer cell motility and tumor growth. In this study, we further characterized if HAI-2 was a cognate inhibitor for matriptase and identified which Kunitz domain of HAI-2 was required for inhibiting matriptase and human prostate cancer cell motility. Our results show that HAI-2 overexpression suppressed matriptase-induced prostate cancer cell motility. We demonstrate that HAI-2 interacts with matriptase on cell surface and inhibits matriptase proteolytic activity. Moreover, cellular HAI-2 harnesses its Kunitz domain 1 (KD1) to inhibit matriptase activation and prostate cancer cell motility although recombinant KD1 and KD2 of HAI-2 both show an inhibitory activity and interaction with matriptase protease domain. The results together indicate that HAI-2 is a cognate inhibitor of matriptase, and KD1 of HAI-2 plays a major role in the inhibition of cellular matritptase activation as well as human prostate cancer invasion. more...
- Published
- 2020
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