1. Synthesis and Biological Evaluation of Amino Chalcone Derivatives as Antiproliferative Agents
- Author
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Sheng-Hui Wang, Qing-Rong Li, Yan-Bing Zhang, Hong-Li Li, Sai-Yang Zhang, Zhao-Yang Mu, Yang-Yang Hu, Yu-Fan Gu, Xiao-Jing Pang, Yin-Ru Li, Jian Song, Qian-Yu Li, Min-Jie Jin, Ting Zhu, and Chao-Fan Lu
- Subjects
Chalcone ,synthesis ,Cell Survival ,Cell ,Pharmaceutical Science ,chalcone ,Antineoplastic Agents ,Apoptosis ,Chemistry Techniques, Synthetic ,01 natural sciences ,Article ,Analytical Chemistry ,lcsh:QD241-441 ,chemistry.chemical_compound ,Inhibitory Concentration 50 ,Structure-Activity Relationship ,lcsh:Organic chemistry ,antiproliferative ,Cell Line, Tumor ,Drug Discovery ,medicine ,Humans ,DAPI ,Physical and Theoretical Chemistry ,Cell Proliferation ,Molecular Structure ,cell apoptosis ,010405 organic chemistry ,Organic Chemistry ,Intrinsic apoptosis ,Biological activity ,In vitro ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,medicine.anatomical_structure ,chemistry ,Biochemistry ,Chemistry (miscellaneous) ,Molecular Medicine ,Drug Screening Assays, Antitumor ,Lead compound - Abstract
Chalcone is a common scaffold found in many biologically active compounds. The chalcone scaffold was also frequently utilized to design novel anticancer agents with potent biological efficacy. Aiming to continue the research of effective chalcone derivatives to treat cancers with potent anticancer activity, fourteen amino chalcone derivatives were designed and synthesized. The antiproliferative activity of amino chalcone derivatives was studied in vitro and 5-Fu as a control group. Some of the compounds showed moderate to good activity against three human cancer cells (MGC-803, HCT-116 and MCF-7 cells) and compound 13e displayed the best antiproliferative activity against MGC-803 cells, HCT-116 cells and MCF-7 cells with IC50 values of 1.52 &mu, M (MGC-803), 1.83 &mu, M (HCT-116) and 2.54 &mu, M (MCF-7), respectively which was more potent than the positive control (5-Fu). Further mechanism studies were explored. The results of cell colony formatting assay suggested compound 10e inhibited the colony formation of MGC-803 cells. DAPI fluorescent staining and flow cytometry assay showed compound 13e induced MGC-803 cells apoptosis. Western blotting experiment indicated compound 13e induced cell apoptosis via the extrinsic/intrinsic apoptosis pathway in MGC-803 cells. Therefore, compound 13e might be a valuable lead compound as antiproliferative agents and amino chalcone derivatives worth further effort to improve amino chalcone derivatives&rsquo, potency.
- Published
- 2020
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