26 results on '"Shuqiang CHEN"'
Search Results
2. The mTORC1 Signaling Support Cellular Metabolism to Dictate Decidual NK Cells Function in Early Pregnancy
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Song Yan, Jie Dong, Chenxi Qian, Shuqiang Chen, Qian Xu, Hui Lei, and Xiaohong Wang
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Mammals ,Abortion, Habitual ,Tumor Necrosis Factor-alpha ,Immunology ,Mechanistic Target of Rapamycin Complex 1 ,Killer Cells, Natural ,Mice ,Pregnancy ,Decidua ,Animals ,Humans ,Immunology and Allergy ,Female ,Signal Transduction - Abstract
Cellular metabolism plays an important role in regulating both human and murine NK cell functions. However, it remains unclear whether cellular metabolic process impacts on the function of decidual NK cells (dNK), essential tissue-resident immune cells maintaining the homeostasis of maternal-fetal interface. Remarkably, we found that glycolysis blockage enhances dNK VEGF-A production but restrains its proliferation. Furthermore, levels of IFN-γ and TNF-α secreted by dNK get decreased when glycolysis or oxidative phosphorylation (OXPHOS) is inhibited. Additionally, glycolysis, OXPHOS, and fatty acid oxidation disruption has little effects on the secretion and the CD107a-dependent degranulation of dNK. Mechanistically, we discovered that the mammalian target of rapamycin complex 1 (mTORC1) signaling inhibition leads to decreased glycolysis and OXPHOS in dNK. These limited metabolic processes are associated with attenuated dNK functions, which include restricted production of cytokines including IFN-γ and TNF-α, diminished CD107a-dependent degranulation, and restrained dNK proliferation. Finally, we reported that the protein levels of several glycolysis-associated enzymes are altered and the mTORC1 activity is significantly lower in the decidua of women with recurrent pregnancy loss (RPL) compared with normal pregnancy, which might give new insights about the pathogenesis of RPL. Collectively, our data demonstrate that glucose metabolism and mTORC1 signaling support dNK functions in early pregnancy.
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- 2022
3. The effect of ovarian stimulation on aneuploidy of early aborted tissues and preimplantation blastocysts: comparison of the GnRH agonist long protocol with the GnRH antagonist protocol
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Jun Wang, Jing Zhang, Nan Zhao, Yuan Ma, Xiyi Wang, Xingqing Gou, Ying Ju, Hengde Zhang, Shuqiang Chen, and Xiaohong Wang
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Male ,Pregnancy Rate ,Obstetrics and Gynecology ,General Medicine ,Fertilization in Vitro ,Aneuploidy ,Abortion, Spontaneous ,Gonadotropin-Releasing Hormone ,Blastocyst ,Hormone Antagonists ,Reproductive Medicine ,Ovulation Induction ,Pregnancy ,Semen ,Genetics ,Humans ,Multicenter Studies as Topic ,Female ,Genetics (clinical) ,Developmental Biology ,Retrospective Studies - Abstract
To compare aneuploidy rates in early aborted tissues or blastocysts between in vitro fertilization (IVF) cycles after the gonadotropin-releasing hormone (GnRH) antagonist (GnRH-ant) protocol or the GnRH agonist (GnRH-a) long protocol.This was a retrospective cohort study from a university-affiliated fertility center. In total, 550 early miscarriage patients who conceived through IVF/intracytoplasmic sperm injection (ICSI) after receiving the GnRH-ant or GnRH-a long protocol were analyzed to compare aneuploidy rates in early aborted tissues. To compare aneuploidy rates in blastocysts, 404 preimplantation genetic testing for aneuploidy (PGT-A) cycles with the GnRH-ant protocol or GnRH-a long protocol were also analyzed.For early miscarriage patients who conceived through IVF/ICSI, compared to the GnRH-a long protocol group, the GnRH-ant protocol group had a significantly higher rate of aneuploidy in early aborted tissues (48.51% vs. 64.19%). Regarding PGT-A cycles, the rate of blastocyst aneuploidy was significantly higher in the GnRH-ant protocol group than the GnRH-a long protocol group (39.69% vs. 52.27%). After stratification and multiple linear regression, the GnRH-ant regimen remained significantly associated with an increased risk of aneuploidy in early aborted tissues and blastocysts [OR (95% CI) 1.81 (1.21, 2.71), OR (95% CI) 1.65 (1.13, 2.42)]. Furthermore, the blastocyst aneuploidy rate in the GnRH-ant protocol group was significantly higher but only in young and normal ovarian responders [OR (95% CI) 5.07 (1.99, 12.92)].Compared to the GnRH-a long protocol, the GnRH-ant protocol is associated with a higher aneuploidy rate in early aborted tissues and blastocysts. These results should be confirmed in a multicenter, randomized controlled trial.
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- 2022
4. Microcystic pattern and shadowing are independent predictors of ovarian borderline tumors and cystadenofibromas in ultrasound
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Xian-Lan Liu, Guorong Lyu, Xiaobin Yan, Wenmin Yang, Fengying Ye, Yaduan Gan, Shuqiang Chen, Maiguo Hu, Xiuming Wu, Ying Zhang, and Xinying Zheng
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medicine.medical_specialty ,Logistic regression ,Malignancy ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,Ovarian tumor ,0302 clinical medicine ,Humans ,Medicine ,Radiology, Nuclear Medicine and imaging ,Cystadenocarcinoma ,Retrospective Studies ,Ultrasonography ,Ovarian Neoplasms ,Univariate analysis ,business.industry ,Ultrasound ,Cystadenofibroma ,General Medicine ,medicine.disease ,Acoustic shadow ,030220 oncology & carcinogenesis ,Cystadenoma ,Female ,Radiology ,business - Abstract
To determine the sonographic characteristics of borderline tumors (BoTs) and cystadenofibromas (CAFs). Preoperative sonograms from consecutive patients who had at least one primary epithelial tumor in the adnexa were retrospectively collected. All tumors were described using the International Ovarian Tumor Analysis terminology. Ultrasound variables were tested using multinomial logistic regression after univariate analysis. A total of 650 patients were included in this study. Of these, 110 had a CAF, 128 had a BoT, 249 had a cystadenoma (CAD), and 163 had a cystadenocarcinoma (CAC). Nearly half of CAFs and more than half of BoTs and CACs appeared to be unilocular and multilocular solid on the ultrasound images, while CADs were predominantly uni- or multilocular (p
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- 2020
5. Discovery of novel bis-evodiamine derivatives with potent antitumor activity
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Huixin, Liang, Wei, Wang, Fugui, Zhu, Shuqiang, Chen, Dan, Liu, and Chunquan, Sheng
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Organic Chemistry ,Clinical Biochemistry ,Pharmaceutical Science ,Antineoplastic Agents ,Apoptosis ,Biochemistry ,Structure-Activity Relationship ,Cell Line, Tumor ,Drug Design ,Drug Discovery ,Quinazolines ,Humans ,Molecular Medicine ,Drug Screening Assays, Antitumor ,Molecular Biology ,Cell Proliferation - Abstract
Inspired by antitumor natural product evodiamine, a series of novel bis-evodiamine derivatives were designed and synthesized, which showed potent antitumor activity. In particular, compound 13b effectively inhibited the proliferation and migration of HCT116 cells. Further mechanism studies revealed that compound 13b acted by inducing HCT116 cell apoptosis and arresting the cell cycle at the G2/M phase. Thus, compound 13b represents a promising lead compound for the discovery of novel antitumor agents.
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- 2022
6. Extra-Thyroid Extension Prediction by Ultrasound Quantitative Method Based on Thyroid Capsule Response Evaluation
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Yiran Gong, Yi-Ming Su, Liya Li, Jinshu Zeng, Weili Wei, Yinan Huang, Shuqiang Chen, Minghang Lin, and Haihong Shi
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Capsular Invasion ,Adult ,Male ,Aspect ratio ,Thyroid Gland ,030204 cardiovascular system & hematology ,Diagnosis, Differential ,03 medical and health sciences ,0302 clinical medicine ,Clinical Research ,medicine ,Carcinoma ,Humans ,Neoplasm Invasiveness ,Thyroid Neoplasms ,Neoplasm Metastasis ,Retrospective Studies ,Ultrasonography ,business.industry ,Ultrasound ,Thyroid ,Capsule ,Nodule (medicine) ,General Medicine ,Middle Aged ,medicine.disease ,Prognosis ,Carcinoma, Papillary ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Female ,medicine.symptom ,Nuclear medicine ,business ,Calcification - Abstract
BACKGROUND The aim of this study was to assess the interaction between thyroid malignancies and thyroid anterior capsule by ultrasound quantification to determine extra-capsular invasion. MATERIAL AND METHODS A total of 145 patients preoperatively diagnosed with malignant nodules under the thyroid anterior capsule were selected and routinely examined by ultrasound. The length of the nodules (from the junction of the nodule capsule to the deepest point of the nodule, vertical diameter, V) and the distance between the nodule protruding from thyroid capsule and the highest protruding (ledge length, L) nodule were used to obtain the L/V ratio. These parameters where then used to compare the efficacy of predicting extra-thyroid extension (ETE) between L/V, the aspect ratio of the tumor, and manual judgment. RESULTS Out of 145 nodules, there were 63 ETEs and 82 non-ETEs determined by ultrasound. Extra-capsular invasion was associated with L//V ratio, but there was no significant correlation between capsular invasion and AR (aspect ratio), age, location, or presence of clustered calcification. The ability of the ratio of L/V to predict extra-capsular invasion was superior to the predictive ability of the AR ratio. With a Youden index of 0.593, the L/V ratio was 0.2325. The use of the L/V ratio to determine the presence of ETE was superior to subjective visual judgment. CONCLUSIONS The calculation of L/V ratio by ultrasound could more precisely predict the ETE compared with manual judgment, which indirectly reflects the interaction between thyroid capsule and malignant nodules. The above conclusions need to be confirmed by a range of cases.
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- 2021
7. Prenatal Evaluation for Detection of Anorectal Atresia: Value of Ultrasound
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Yi-Ming Su, Jin-Rong Lin, Yan Lin, Guorong Lv, Hong-Yi Yang, and Shuqiang Chen
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medicine.medical_specialty ,Anal Canal ,Rectum ,Prenatal diagnosis ,Ultrasonography, Prenatal ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,Pregnancy ,otorhinolaryngologic diseases ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Prospective Studies ,Anorectal atresia ,Fetus ,030219 obstetrics & reproductive medicine ,Radiological and Ultrasound Technology ,business.industry ,Ultrasound ,Reproducibility of Results ,Gestational age ,Anal canal ,medicine.disease ,Anorectal Malformations ,medicine.anatomical_structure ,Female ,Radiology ,Imperforate anus ,business - Abstract
Objectives To investigate the applicability and value of ultrasound (US) in the diagnosis of anorectal atresia. Methods Between January 2008 and January 2016, we prospectively evaluated 63,101 fetuses (gestational age, 20-38 weeks), including low- and high-risk populations using 2-dimensional US scans. An abnormal imaging finding was defined as an anal canal diameter of less than the 95% confidence interval (small anal canal) of the normal range or the absence of an anal canal and rectum. Imaging findings were considered normal on detection of an anal canal with a normal width and the absence of abnormalities. Prenatal imaging findings were confirmed by a postnatal or postmortem examination. Results Among the investigated fetuses, 28 showed evidence of anorectal atresia on US scans, and 22 of those with anorectal atresia had additional anomalies. Six cases of isolated anorectal atresia were successfully detected during the preclusive prenatal US scans. Four cases of a low imperforate anus (including 2 covered anuses) yielded false-negative results, indicating a diagnostic rate of 87.5% (28 of 32). The normal appearance of the fetal rectum and anal canal ruled out anorectal atresia in 30 fetuses with a dilated colon. Additionally, there were 3 false-positive cases, in which a narrow anal canal was observed. Conclusions Identifying the abnormal appearance or absence of the fetal anal canal and rectum on preclusive US anomaly scans is useful for prenatal diagnosis or exclusion of anorectal atresia, which may help improve the detection of isolated anorectal atresia. Furthermore, a combined evaluation of the longitudinal and axial appearances of the fetal anal canal and rectum can improve diagnostic accuracy.
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- 2018
8. Placental diseases associated with assisted reproductive technology
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Xudong Zhang, Meng Xiang, Shuqiang Chen, and Yuan Ma
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0301 basic medicine ,Placenta Diseases ,Reproductive Techniques, Assisted ,medicine.medical_treatment ,Physiology ,Fetal heart ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Pregnancy ,Placenta ,Medicine ,Humans ,Epigenetics ,Fetus ,030219 obstetrics & reproductive medicine ,Assisted reproductive technology ,business.industry ,030104 developmental biology ,Increased risk ,medicine.anatomical_structure ,Placental Dysplasia ,Animal Science and Zoology ,Female ,business ,Genomic imprinting ,Developmental Biology - Abstract
The placenta develops from the outer trophoblastic layer following the differentiation of the fertilized ovum and is therefore more susceptible to epigenetic regulatory changes caused by environmental interventions and influences during assisted reproductive technology. Furthermore, the placenta regulates the development of the fetal heart, brain, kidneys, bones, and other tissues and organs [1]. Placental dysplasia leads to poor perinatal outcomes as well as long-term health risks later in life, including neurodevelopmental disorders, tumors, and adult metabolic syndrome [2,3]. In view of the decisive role of the placenta during intrauterine fetal development, Graham J. Burton, an expert in placentology from the University of Cambridge, formally proposed the theory of "placenta-derived chronic diseases" in 2018 based on embryonic-derived diseases [4]. In this review, we summarized the changes in placental morphology and structure, growth dynamics, imprinted and non-imprinted genes, and other aspects attributable to assisted reproduction technology. Our review provides a theoretical basis for further research on placental changes caused by assisted reproductive technology that are most strongly associated with an increased risk of neonatal long-term diseases.
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- 2021
9. High-coverage targeted lipidomics revealed dramatic lipid compositional changes in asthenozoospermic spermatozoa and inverse correlation of ganglioside GM3 with sperm motility
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Li Li, Bin Kang, Xu-Hui Ma, Jun Wang, Ming Wang, Shuqiang Chen, Hengde Zhang, Bo Li, Jianlei Huang, and Yang Cai
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0301 basic medicine ,Adult ,Male ,GM3 ,endocrine system ,QH471-489 ,Motility ,Asthenozoospermia ,Andrology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Endocrinology ,Lipidomics ,medicine ,G(M3) Ganglioside ,Humans ,Inverse correlation ,Sperm motility ,reproductive and urinary physiology ,030219 obstetrics & reproductive medicine ,Chemistry ,Cholesterol ,urogenital system ,Reproduction ,Research ,Obstetrics and Gynecology ,Gynecology and obstetrics ,medicine.disease ,Lipid Metabolism ,Sperm ,Lipids ,Spermatozoa ,Ganglioside GM3 ,030104 developmental biology ,Reproductive Medicine ,RG1-991 ,lipids (amino acids, peptides, and proteins) ,Developmental Biology - Abstract
Background It has been previously demonstrated that cholesterol content and cholesterol/phospholipid ratio were significantly higher in asthenozoospermia and oligoasthenoteratozoospermia. The majority of published studies have investigated the fatty acid composition of phospholipids rather than lipids themselves. This study evaluated the lipid composition of asthenozoospermic and normozoospermic spermatozoa, and identified the exact lipid species that correlated with sperm motility. Methods A total of 12 infertile asthenozoospermia patients and 12 normozoospermia subjects with normal sperm motility values were tested for semen volume, sperm concentration, count, motility, vitality and morphology. High-coverage targeted lipidomics with 25 individual lipid classes was performed to analyze the sperm lipid components and establish the exact lipid species that correlated with sperm motility. Results A total of 25 individual lipid classes and 479 lipid molecular species were identified and quantified. Asthenozoospermic spermatozoa showed an increase in the level of four lipid classes, including Cho, PE, LPI and GM3. A total of 48 lipid molecular species were significantly altered between normozoospermic and asthenozoospermic spermatozoa. Furthermore, the levels of total GM3 and six GM3 molecular species, which were altered in normozoospermic spermatozoa versus asthenozoospermic spermatozoa, were inversely correlated with sperm progressive and total motility. Conclusions Several unique lipid classes and lipid molecular species were significantly altered between asthenozoospermic and normozoospermic spermatozoa, revealing new possibilities for further mechanistic pursuits and highlighting the development needs of culture medium formulations to improve sperm motility.
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- 2020
10. Scaffold Hopping of Natural Product Evodiamine: Discovery of a Novel Antitumor Scaffold with Excellent Potency against Colon Cancer
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Yu Li, Lei Wang, Shuqiang Chen, Chunquan Sheng, Junfei Cheng, Guoqiang Dong, Yahui Huang, Shanchao Wu, and Kun Fang
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G2 Phase ,Scaffold ,Antineoplastic Agents ,Apoptosis ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,Structure-Activity Relationship ,In vivo ,Evodiamine ,Cell Line, Tumor ,Drug Discovery ,Structure–activity relationship ,Animals ,Humans ,Topoisomerase II Inhibitors ,030304 developmental biology ,0303 health sciences ,Natural product ,biology ,Topoisomerase ,Cell Cycle ,HCT116 Cells ,Xenograft Model Antitumor Assays ,Tubulin Modulators ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Tubulin ,chemistry ,Cell culture ,Drug Design ,Colonic Neoplasms ,biology.protein ,Cancer research ,Quinazolines ,Molecular Medicine ,Topoisomerase I Inhibitors - Abstract
Inspired by the natural product evodiamine, a novel antitumor indolopyrazinoquinazolinone scaffold was designed by scaffold hopping. Structure-activity relationship studies led to the discovery of compound 15j, which shows low nanomolar inhibitory activity against the HCT116 cell line. Further antitumor mechanism studies indicated that compound 15j acted by the dual inhibition of topoisomerase 1 and tubulin and induced apoptosis with G2 cell-cycle arrest. The quaternary ammonium salt of compound 15j (compound 15js) exhibited excellent in vivo antitumor activity (TGI = 66.6%) in the HCT116 xenograft model with low toxicity. Indolopyrazinoquinazolinone derivatives represent promising multitargeting antitumor leads for the development of novel antitumor agents.
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- 2019
11. Novel non-peptidic small molecule inhibitors of secreted aspartic protease 2 (SAP2) for the treatment of resistant fungal infections
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Jie Tu, Guoqiang Dong, Na Liu, Ying Wu, Yang Liu, Shuqiang Chen, and Chunquan Sheng
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0301 basic medicine ,Antifungal ,Antifungal Agents ,Aspartic Acid Proteases ,Virulence Factors ,medicine.drug_class ,Antifungal drug ,Catalysis ,Virulence factor ,Microbiology ,Fungal Proteins ,Structure-Activity Relationship ,03 medical and health sciences ,Aspartate protease ,Candida albicans ,Materials Chemistry ,medicine ,Animals ,Humans ,Potency ,Structure–activity relationship ,Protease Inhibitors ,Caenorhabditis elegans ,Mice, Inbred ICR ,biology ,Chemistry ,Candidiasis ,Metals and Alloys ,General Chemistry ,biology.organism_classification ,Small molecule ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Molecular Docking Simulation ,030104 developmental biology ,Ceramics and Composites ,Thiazolidines ,Female - Abstract
Targeting secreted aspartic protease 2 (SAP2), a kind of virulence factor, represents a new strategy for antifungal drug discovery. In this report, the first-generation of small molecule SAP2 inhibitors was rationally designed and optimized using a structure-based approach. In particular, inhibitor 23h was highly potent and selective and showed good antifungal potency for the treatment of resistant Candida albicans infections.
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- 2018
12. Design, synthesis and biological evaluation of novel antitumor spirotetrahydrothiopyran–oxindole derivatives as potent p53-MDM2 inhibitors
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Shuqiang Chen, Chunquan Sheng, Yan Jiang, Shipeng He, Changjin Ji, Shengzheng Wang, Jian Li, and Zhenyuan Miao
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Indoles ,Clinical Biochemistry ,Pharmaceutical Science ,Antineoplastic Agents ,Apoptosis ,Fluorescence Polarization ,01 natural sciences ,Biochemistry ,Structure-Activity Relationship ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Drug Discovery ,Tumor Cells, Cultured ,Humans ,Spiro Compounds ,Oxindole ,Molecular Biology ,Cell Proliferation ,Pyrans ,Antitumor activity ,Dose-Response Relationship, Drug ,Molecular Structure ,biology ,010405 organic chemistry ,Organic Chemistry ,Enantioselective synthesis ,Proto-Oncogene Proteins c-mdm2 ,Combinatorial chemistry ,Oxindoles ,0104 chemical sciences ,chemistry ,Drug development ,Drug Design ,030220 oncology & carcinogenesis ,Cancer cell ,biology.protein ,Molecular Medicine ,Mdm2 ,Drug Screening Assays, Antitumor ,Tumor Suppressor Protein p53 ,Lead compound - Abstract
p53–MDM2 protein-protein interaction is a promising target for novel antitumor drug development. Previously, we identified a new class of spirotetrahydrothiopyran–oxindole p53–MDM2 inhibitors by novel organocatalytic enantioselective cascade reactions. Herein, a series of new derivatives were designed, synthesized and assayed to investigate the structure-activity relationships. Among them, compound B14 bearing a novel spiroindole–thiopyranopyridone scaffold exhibited potent MDM2 inhibitory activity as well as antitumor activity, which could effectively induce the apoptosis of A549 cancer cells. It represents a promising lead compound for the development of novel antitumor agents.
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- 2017
13. Water-soluble derivatives of evodiamine: Discovery of evodiamine-10-phosphate as an orally active antitumor lead compound
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Guoqiang Dong, Chunquan Sheng, Yahui Huang, Yu Li, Shuqiang Chen, Shanchao Wu, and Kaijian Bi
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Administration, Oral ,Mice, Nude ,Antineoplastic Agents ,Apoptosis ,Pharmacology ,01 natural sciences ,Phosphates ,Mice ,Structure-Activity Relationship ,03 medical and health sciences ,chemistry.chemical_compound ,Evodiamine ,Drug Discovery ,Animals ,Humans ,Potency ,Cell Proliferation ,030304 developmental biology ,Mice, Inbred BALB C ,0303 health sciences ,Dose-Response Relationship, Drug ,Molecular Structure ,biology ,010405 organic chemistry ,Organic Chemistry ,Water ,Neoplasms, Experimental ,General Medicine ,HCT116 Cells ,Phosphate ,0104 chemical sciences ,Tubulin ,Water soluble ,Orally active ,DNA Topoisomerases, Type I ,Solubility ,chemistry ,Quinazolines ,biology.protein ,Female ,Drug Screening Assays, Antitumor ,Lead compound - Abstract
10-Hydroxyevodiamine is a multitargeting antitumor lead compound with excellent in vitro activity. However, its in vivo antitumor potency is rather limited, which has hampered its further clinical development. To overcome this obstacle, a series of novel water-soluble derivatives of 10-hydroxyevodiamine were designed and synthesized. Most of them exhibited good to excellent antitumor activities against several cancer cell lines. In particular, phosphate derivative 9 was orally active and showed improved in vivo antitumor efficacy in HCT116 xenograft models. Further antitumor mechanism studies indicated that compound 9 acted by triple Top1/Top2/tubulin inhibition and induced apoptosis with G2/M cell cycle arrest. Taken together, this study extended the structure-activity relationship of evodiamine and identified phosphate derivative 9 as a promising antitumor lead compound.
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- 2021
14. Structural simplification of evodiamine: Discovery of novel tetrahydro-β-carboline derivatives as potent antitumor agents
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Yahui Huang, Kun Wan, Fugui Zhu, Chunquan Sheng, Dan Liu, Guoqiang Dong, Shuqiang Chen, and Zonglin Ma
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Male ,β carboline derivatives ,Clinical Biochemistry ,Mice, Nude ,Pharmaceutical Science ,Antineoplastic Agents ,Apoptosis ,Proof of Concept Study ,01 natural sciences ,Biochemistry ,Structure-Activity Relationship ,chemistry.chemical_compound ,In vivo ,Evodiamine ,Neoplasms ,Drug Discovery ,Animals ,Humans ,Topoisomerase II Inhibitors ,Structure–activity relationship ,Molecular Biology ,Cell Proliferation ,Antitumor activity ,Molecular Structure ,010405 organic chemistry ,Organic Chemistry ,HCT116 Cells ,Xenograft Model Antitumor Assays ,Combinatorial chemistry ,0104 chemical sciences ,G2 Phase Cell Cycle Checkpoints ,010404 medicinal & biomolecular chemistry ,Drug development ,chemistry ,Quinazolines ,Molecular Medicine ,Drug Screening Assays, Antitumor ,Topoisomerase I Inhibitors ,Lead compound ,Carbolines - Abstract
Natural products (NPs) have played a crucial role in the discovery and development of antitumor drugs. However, the high structural complexity of NPs generally results in unfavorable physicochemical profiles and poor drug-likeness. A powerful strategy to tackle this obstacle is the structural simplification of NPs by truncating nonessential structures. Herein, a series of tetrahydro-β-carboline derivatives were designed by elimination of the D ring of NP evodiamine. Structure-activity relationship studies led to the discovery of compound 45, which displayed highly potent antitumor activity against all the tested cancer cell lines and excellent in vivo antitumor activity in the HCT116 xenograft model with low toxicity. Further mechanistic research indicated that compound 45 acted by dual Top1/2 inhibition and induced caspase-dependent cell apoptosis coupled with G2/M cell cycle arrest. This proof-of-concept study validated the effectiveness of structural simplification in NP-based drug development, discovered compound 45 as a potent antitumor lead compound and enriched the structure-activity relationships of evodiamine.
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- 2021
15. Ultrasound Versus Contrast-Enhanced Magnetic Resonance Imaging for Subclinical Synovitis and Tenosynovitis: A Diagnostic Performance Study
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Zhongtao Bao, Yanchun Zhao, Shuqiang Chen, Xiaoyu Chen, Xiang Xu, Linglin Wei, and Meilian Xiong
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Adult ,Male ,Wrist Joint ,Medicine (General) ,medicine.medical_specialty ,Radiography ,030204 cardiovascular system & hematology ,Wrist ,03 medical and health sciences ,R5-920 ,0302 clinical medicine ,Synovitis ,Ultrasound ,medicine ,Humans ,030212 general & internal medicine ,Proximal Interphalangeal Joints ,Aged ,Ultrasonography ,Tenosynovitis ,medicine.diagnostic_test ,business.industry ,Magnetic resonance imaging ,General Medicine ,Metacarpophalangeal Joints ,Middle Aged ,medicine.disease ,Magnetic Resonance Imaging ,medicine.anatomical_structure ,Rheumatoid arthritis ,Joint pain ,Female ,Original Article ,Radiology ,medicine.symptom ,business - Abstract
OBJECTIVES: Radiographic manifestations of synovitis (e.g., erosions) can be observed only in the late stage of rheumatoid arthritis. Ultrasound is a noninvasive, cheap, and widely available technique that enables the evaluation of inflammatory changes in the peripheral joint. In the same way, dynamic contrast-enhanced magnetic resonance imaging (MRI) enables qualitative and quantitative measurements. The objectives of the study were to compare the sensitivity and accuracy of ultrasound in detecting subclinical synovitis and tenosynovitis with those of contrast-enhanced MRI. METHODS: The ultrasonography and contrast-enhanced MRI findings of the wrist, metacarpophalangeal, and proximal interphalangeal joints (n=450) of 75 patients with a history of joint pain and morning stiffness between 6 weeks and 2 years were reviewed. The benefits score was evaluated for each modality. RESULTS: The ultrasonic findings showed inflammation in 346 (77%) joints, while contrast-enhanced MRI found signs of early rheumatoid arthritis in 372 (83%) joints. The sensitivities of ultrasound and contrast-enhanced MRI were 0.795 and 0.855, respectively, and the accuracies were 0.769 and 0.823, respectively. Contrast-enhanced MRI had a likelihood of 0-0.83 and ultrasound had a likelihood of 0-0.77 for detecting synovitis and tenosynovitis at one time. The two imaging modalities were equally competitive for detecting synovitis and tenosynovitis (p=0.055). CONCLUSION: Ultrasound could be as sensitive and specific as contrast-enhanced MRI for the diagnosis of subclinical synovitis and tenosynovitis.
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- 2019
16. Quantifying statistical uncertainty in metrics of sleep disordered breathing
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Robert Thomas, Uri T. Eden, Shuqiang Chen, and Michael J. Prerau
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Male ,Polysomnography ,Population ,Eligibility Determination ,Comorbidity ,Poisson distribution ,03 medical and health sciences ,symbols.namesake ,0302 clinical medicine ,Sleep Apnea Syndromes ,Statistical significance ,Statistics ,Medicine ,Humans ,Point estimation ,Medical diagnosis ,education ,Aged ,education.field_of_study ,Models, Statistical ,business.industry ,Confounding ,Uncertainty ,Sleep apnea ,General Medicine ,medicine.disease ,Confidence interval ,030228 respiratory system ,symbols ,Female ,business ,030217 neurology & neurosurgery - Abstract
Background The apnea-hypopnea index (AHI) (or one of its derivatives) is the primary clinical metric for characterizing sleep disordered breathing—the value of which with respect to a threshold determines severity of diagnosis and eligibility for treatment reimbursement. The index value, however, is taken as a perfect point estimate, with no measure of statistical uncertainty. Thus, current practice does not robustly account for variability in diagnosis/eligibility due to chance. In this paper, we quantify the statistical uncertainty associated with respiratory event indices for sleep disordered breathing and the effect of uncertainty on treatment eligibility. Methods We develop an empirical estimate of uncertainty using a non-parametric bootstrap on the interevent times, as well as a theoretical Poisson estimate reflecting the current formulation of the AHI. We then apply these methods to estimate AHI uncertainty for 2049 subjects (954/1095 M/F, age: mean 69 ± 9.1) from the Multi-Ethnic Study of Atherosclerosis (MESA). Results and Conclusions The mean 95% empirical confidence interval width was 11.500 ± 6.208 events per hour and the mean 95% theoretical Poisson confidence interval width was 5.998 ± 2.897 events per hour, suggesting that uncertainty is likely a major confounding factor within the current diagnostic framework. Of the 278 subjects in the symptomatic population (ESS>10), 27% (76/278) had uncertain diagnoses given the 95% empirical confidence interval. Of the 2049 subjects in the full population, 43% (880/2049) had uncertain diagnoses given the 95% empirical confidence interval. The inclusion of subjects with uncertain diagnoses increases the number of eligible patients by 21.3% for the symptomatic population and by 84.8% for the full population. The exclusion of subjects with uncertain diagnoses given the 95% empirical confidence interval decreases the number of eligible patients by 12.4% for the symptomatic population and by 34.8% for full population. Additional analyses suggest that it is practically infeasible to gain diagnostic statistical significance through additional testing for a broad range of borderline cases. Overall, these results suggest that AHI uncertainty is a vital additional piece of information that would greatly benefit clinical practice, and that the inclusion of uncertainty in epidemiological analysis might help improve the ability for researchers to robustly link AHI with co-morbidities and long-term outcomes.
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- 2019
17. Design, synthesis and biological evaluation of novel antitumor spirodihydrothiopyran-oxindole derivatives
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Shipeng He, Chunquan Sheng, Shengzheng Wang, Wei Liu, Fan Zhang, and Shuqiang Chen
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Clinical Biochemistry ,Pharmaceutical Science ,01 natural sciences ,Biochemistry ,chemistry.chemical_compound ,Structure-Activity Relationship ,Drug Discovery ,Humans ,Oxindole ,Spiro Compounds ,Molecular Biology ,Biological evaluation ,Antitumor activity ,biology ,010405 organic chemistry ,Drug discovery ,Chemistry ,Organic Chemistry ,Cell cycle ,In vitro ,0104 chemical sciences ,Oxindoles ,010404 medicinal & biomolecular chemistry ,biology.protein ,Molecular Medicine ,Mdm2 ,Lead compound - Abstract
Sulfur containing spiroheterocyclic oxindoles are promising privileged scaffolds in medicinal chemistry and drug discovery. Previously, we identified a new class of spirodihydrothiopyran-oxindoles with good in vitro antitumor activity against A549 lung cancer cell line. Herein, various spirooxindole-dihydrothiopyrans with diverse substitutions were synthesized and assayed to investigate the structure-activity relationships. Among the derivatives, compounds 4b, 4i, 4m, 4n and 4q displayed superior or comparable antitumor activity than nutlin-3. Molecular mechanism study revealed this scaffold displayed moderate MDM2 inhibitory activity, significantly induced cancer cell apoptosis and arrested cell cycle at G0/G1 phase, which represented a good lead compound for antitumor drug discovery.
- Published
- 2019
18. Supraphysiological serum oestradiol negatively affects birthweight in cryopreserved embryo transfers: a retrospective cohort study
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Yujing Xiong, Yefei Ma, Shuqiang Chen, Xifeng Xiao, Wanlin Zhang, and Xiaohong Wang
- Subjects
0301 basic medicine ,Adult ,Fertilization in Vitro ,Cryopreservation ,Andrology ,Embryo Culture Techniques ,Tertiary Care Centers ,03 medical and health sciences ,0302 clinical medicine ,Ovulation Induction ,Negatively associated ,Pregnancy ,Medicine ,Birth Weight ,Humans ,reproductive and urinary physiology ,Retrospective Studies ,030219 obstetrics & reproductive medicine ,Estradiol ,Multivariable regression analysis ,business.industry ,Pregnancy Outcome ,Obstetrics and Gynecology ,Embryo ,Retrospective cohort study ,Embryo Transfer ,Vitrification ,Confidence interval ,Embryo transfer ,030104 developmental biology ,Reproductive Medicine ,Cohort ,Female ,business ,Infertility, Female ,hormones, hormone substitutes, and hormone antagonists ,Developmental Biology - Abstract
Research question Previous studies have demonstrated a negative relationship between peak oestradiol and low birthweight in IVF. However, it is hard to distinguish which aspect influenced by supraphysiological oestradiol concentrations fundamentally contributes to the low birthweight seen during IVF. This study therefore aimed to investigate whether birthweight was associated with an effect of oestradiol on oocytes. Design Oocytes are the only component exposed to the high-oestradiol environment in vitrified-warmed embryo transfer (VET) cycles. A retrospective cohort study of 431 infertile women was therefore carried out to evaluate the relationship between peak oestradiol concentration during controlled ovarian stimulation (COS) and birthweight in full-term singletons born after VET. The effect size was calculated using multivariable regression analysis. Results In this cohort, the mean peak oestradiol concentration was 4186.6 ± 1215.0 pg/ml, the mean number of oocytes retrieved was 11.5 ± 5.4, the mean length of ovarian stimulation was 11.3 ± 2.1 days and the mean birthweight was 3441.8 ± 466.1 g. The results indicated that peak oestradiol concentration was negatively correlated with birthweight in full-term singletons born after VET (adjusted β –5.0, 95% confidence interval [CI] –9.2 to –0.7). The effect size indicated that for every 100 pg/ml increase in peak oestradiol concentration, birthweight decreased by 5.0 g. The P for trend value was 0.038. Conclusions Peak serum oestradiol during COS is negatively associated with birthweight. This model proposes a novel concept as well as new evidence that the effect on birthweight is due to the primary influence of a high oestradiol concentration on oocytes during COS.
- Published
- 2018
19. Synthesis of spiro-tetrahydrothiopyran-oxindoles by Michael-aldol cascade reactions: discovery of potential P53-MDM2 inhibitors with good antitumor activity
- Author
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Shengzheng Wang, Chunquan Sheng, Shengyong Zhang, Xueying Liu, Fan Zhang, Zhongjie Guo, Shipeng He, Shuqiang Chen, and Weiping Chen
- Subjects
Proline ,Stereoisomerism ,Antineoplastic Agents ,010402 general chemistry ,01 natural sciences ,Biochemistry ,P53 mdm2 ,Catalysis ,Cascade reaction ,Aldol reaction ,Cell Line, Tumor ,Humans ,Spiro Compounds ,Physical and Theoretical Chemistry ,Enzyme Inhibitors ,Antitumor activity ,Binding Sites ,010405 organic chemistry ,Chemistry ,Drug discovery ,Organic Chemistry ,Proto-Oncogene Proteins c-mdm2 ,Combinatorial chemistry ,0104 chemical sciences ,Oxindoles ,Molecular Docking Simulation ,Tumor Suppressor Protein p53 - Abstract
Using proline as the catalyst, an organocatalytic Michael–aldol cascade reaction was developed for the synthesis of spiro-tetrahydrothiopyran oxindoles. The highly functionalized scaffold was assembled in moderate to good yields (51–78%) and excellent diastereoselectivities (>20 : 1 dr). Interestingly, the oxindoles displayed moderate to good in vitro antitumor activities and were validated as p53-MDM2 inhibitors, which represented promising lead compounds for antitumor drug discovery.
- Published
- 2018
20. Discovery of IDO1 and DNA dual targeting antitumor agents
- Author
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Shuqiang Chen, Chunquan Sheng, Kun Fang, Jianhe Liu, Guoqiang Dong, Wei Wang, Shanchao Wu, and Ying Wu
- Subjects
0301 basic medicine ,Melphalan ,medicine.medical_treatment ,Antineoplastic Agents ,Pharmacology ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,Structure-Activity Relationship ,0302 clinical medicine ,Cancer immunotherapy ,Cell Line, Tumor ,Drug Discovery ,medicine ,Structure–activity relationship ,Animals ,Humans ,Indoleamine-Pyrrole 2,3,-Dioxygenase ,Physical and Theoretical Chemistry ,Enzyme Inhibitors ,Cell Proliferation ,Dose-Response Relationship, Drug ,Molecular Structure ,Drug discovery ,Organic Chemistry ,Tryptophan ,Immunotherapy ,DNA, Neoplasm ,Neoplasms, Experimental ,Small molecule ,030104 developmental biology ,chemistry ,030220 oncology & carcinogenesis ,Pharmacophore ,Drug Screening Assays, Antitumor ,DNA ,medicine.drug - Abstract
The development of small molecules for cancer immunotherapy is highly challenging and indoleamine 2,3-dioxygenase 1 (IDO1) represents a promising target. Inspired by the synergistic effects between IDO1 inhibitors and traditional antitumor chemotherapeutics, the first orally active dual IDO1 and DNA targeting agents were designed by the pharmacophore fusion strategy. The bifunctional hybrids exhibited enhanced IDO1 enzyme inhibitory activity and in vitro cytotoxicity as compared to IDO1 inhibitor 1-methyl-tryptophan and DNA alkylating agent melphalan. In a murine LLC tumor model, the dual targeting agents demonstrated excellent antitumor efficacy, highlighting the advantages of this novel design strategy to improve the efficacy of small molecule cancer immunotherapy.
- Published
- 2017
21. Plasma exchange parameter selection and safety observation of children with severe ricinism
- Author
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X J Nie, Z H Yu, Y Y Luo, H X Zhan, Z Li, Y T Lu, Z F Weng, Z W Sun, G M Chen, Y Y Yang, S R Zheng, H Q Cao, C F Wang, and Shuqiang Chen
- Subjects
Male ,Mean arterial pressure ,Respiratory rate ,Thrombin Time ,Ricin ,Thrombin time ,Blood plasma ,Heart rate ,Genetics ,Humans ,Medicine ,Child ,Blood Coagulation ,Molecular Biology ,Plasma Exchange ,medicine.diagnostic_test ,business.industry ,General Medicine ,Heparin ,Blood flow ,Castor Bean ,Anesthesia ,Female ,Partial Thromboplastin Time ,business ,medicine.drug ,Partial thromboplastin time - Abstract
The aim of this study was to investigate the selection of plasma exchange (PE) parameters and the safety of children with severe ricinism. The PE parameters and heparin dosage in 7 children with severe ricinism were recorded, and changes in the patients' vital signs and coagulation function were monitored before and after PE. All patients successfully completed PE. The speed of blood flow was 50-80 mL/min, speed of exchange flow was 600-800 mL/h, and isolating rate of blood plasma was 12.5-19.05%. Transmembrane pressure was stable at approximately 100 mmHg, and venous pressure was stable at approximately 95 mmHg. The first dose of heparin was 0.39 ± 0.04 mg/kg, and the maintaining heparin dose was 0.40 ± 0.05 to 0.22 ± 0.03 mg·kg(-1)·h(-1). During the PE process, mean arterial pressure, heart rate, respiratory rate, and pulse oxygen saturation were steady. After PE, the activated partial thromboplastin time and thrombin time prolonged to 2-3 times greater than that before PE. However, no bleeding tendency was seen. For children with severe ricinism, the choice of PE to eliminate the toxin from blood, tissues, and organs was safe and effective.
- Published
- 2015
22. Application of ultrasound technology in the study of ischemic postconditioning to protect testes from ischemia-reperfusion injury
- Author
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Yu Zhang, Shuqiang Chen, Qiu-Yue Chen, Y. Wang, En-Sheng Xue, and Rongxi Liang
- Subjects
Male ,Testicular tissue ,Ischemia ,Apoptosis ,Sensitivity and Specificity ,Testicular Diseases ,Group B ,Testis ,Biopsy ,In Situ Nick-End Labeling ,Genetics ,Animals ,Humans ,Medicine ,Ultrasonography, Doppler, Color ,Ischemic Postconditioning ,Molecular Biology ,medicine.diagnostic_test ,Caspase 3 ,business.industry ,Ultrasound ,Reproducibility of Results ,General Medicine ,medicine.disease ,Disease Models, Animal ,Proto-Oncogene Proteins c-bcl-2 ,Reperfusion Injury ,Anesthesia ,Rabbits ,Ultrasonography ,business ,Perfusion ,Reperfusion injury - Abstract
This study was designed to investigate the application of ultrasound technology in the study of ischemic postconditioning to protect testes from ischemia-reperfusion injury. Seventy-two big white rabbits were divided into mild ischemic groups (Group A: A0, A1, A2, A3), moderate ischemic groups (Group B: B0, B1, B2, B3) under ultrasound monitor, and control group (N = 8). Groups A0 and B0 received direct perfusion, while the other groups received a different short time filling/stopped filling treatment (15 s/15 s, 30 s/30 s, or 45 s/45 s) three times before complete perfusion. Each group received contrast-enhanced ultrasound before complete filling. At 3 days after perfusion, the testicular tissue was removed for biopsy. The parameters of testicular contrast in pre-reperfusion groups A and B differed significantly from those of their corresponding control groups (P0.05). The changes in testis-related pathological indicators in groups A1 and A2 were more significant than those of group A0 (P0.05), and changes in group B2 were more obvious than those of group B0 (P0.05). There were no statistically significant differences in the comparison of other indicators between the corresponding groups (P0.05). Ultrasound technology can help build different degree models of ischemic testes and predict the protective effect of post-ischemic treatment.
- Published
- 2014
23. Novel spiropyrazolone antitumor scaffold with potent activity: Design, synthesis and structure-activity relationship
- Author
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Yu Li, Chunquan Sheng, Wannian Zhang, Na Liu, Yongqiang Zhang, Guoqiang Dong, Guixia Xu, Hua Su, Chao-Yu Miao, Shanchao Wu, and Shuqiang Chen
- Subjects
Scaffold ,Stereochemistry ,Bioactive molecules ,Phenotypic screening ,Antineoplastic Agents ,Apoptosis ,010402 general chemistry ,Crystallography, X-Ray ,01 natural sciences ,Broad spectrum ,Structure-Activity Relationship ,Cell Line, Tumor ,Drug Discovery ,Structure–activity relationship ,Humans ,Spiro Compounds ,Pyrazolones ,Cell Proliferation ,Pharmacology ,Antitumor activity ,Dose-Response Relationship, Drug ,Molecular Structure ,010405 organic chemistry ,Chemistry ,Organic Chemistry ,General Medicine ,Combinatorial chemistry ,0104 chemical sciences ,Design synthesis ,Cancer cell apoptosis ,Drug Design ,Drug Screening Assays, Antitumor - Abstract
Phenotypic screening of high quality compound library is an effective strategy to discover novel bioactive molecules. Previously, we developed the divergent organocatalytic cascade approach to efficiently construct a focused library with scaffold diversity and successfully identified a novel spiropyrazolone antitumor scaffold. Herein, a series of spiropyrazolone derivatives were designed, synthesized and assayed. Most of them showed good in vitro antitumor activity with a broad spectrum. Preliminary structure–activity relationship for the substitutions and the stereo configuration were obtained. Compound 5k showed good antitumor activity and could effectively induce cancer cell apoptosis, which represents a good starting point for the development of novel antitumor agents.
- Published
- 2015
24. Scaffold Diversity Inspired by the Natural Product Evodiamine: Discovery of Highly Potent and Multitargeting Antitumor Agents
- Author
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Shuqiang Chen, Guoqiang Dong, Jianzhong Yao, Kun Fang, Zhenyuan Miao, Chunquan Sheng, Jian Li, Wannian Zhang, Na Liu, and Shengzheng Wang
- Subjects
Drug ,Models, Molecular ,media_common.quotation_subject ,Apoptosis ,Evodia ,chemistry.chemical_compound ,Structure-Activity Relationship ,Evodiamine ,In vivo ,Cell Line, Tumor ,Drug Discovery ,Structure–activity relationship ,Humans ,Topoisomerase II Inhibitors ,media_common ,Biological Products ,Natural product ,biology ,Drug discovery ,Topoisomerase ,Cell Cycle ,Combinatorial chemistry ,Antineoplastic Agents, Phytogenic ,Chemical space ,Tubulin Modulators ,chemistry ,Drug Design ,biology.protein ,Quinazolines ,Molecular Medicine ,Drug Screening Assays, Antitumor ,Topoisomerase I Inhibitors - Abstract
A critical question in natural product-based drug discovery is how to translate the product into drug-like molecules with optimal pharmacological properties. The generation of natural product-inspired scaffold diversity is an effective but challenging strategy to investigate the broader chemical space and identify promising drug leads. Extending our efforts to the natural product evodiamine, a diverse library containing 11 evodiamine-inspired novel scaffolds and their derivatives were designed and synthesized. Most of them showed good to excellent antitumor activity against various human cancer cell lines. In particular, 3-chloro-10-hydroxyl thio-evodiamine (66c) showed excellent in vitro and in vivo antitumor efficacy with good tolerability and low toxicity. Antitumor mechanism and target profiling studies indicate that compound 66c is the first-in-class triple topoisomerase I/topoisomerase II/tubulin inhibitor. Overall, this study provided an effective strategy for natural product-based drug discovery.
- Published
- 2015
25. Identification of candidate biomarkers for the prediction of gestational diabetes mellitus in the early stages of pregnancy using iTRAQ quantitative proteomics
- Author
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Yan Wei, Shuqiang Chen, Liming Shen, Yi Liang, Danqing Zhao, Haorong Wu, Jiaming Xie, and Youjiao Chen
- Subjects
Adult ,Proteomics ,0301 basic medicine ,Apolipoprotein E ,endocrine system diseases ,Clinical Biochemistry ,Quantitative proteomics ,Physiology ,Mass Spectrometry ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,Pregnancy ,Humans ,Medicine ,Trypsin ,Fibrinogen alpha chain ,Receiver operating characteristic ,business.industry ,Blood Proteins ,medicine.disease ,Gestational diabetes ,Diabetes, Gestational ,030104 developmental biology ,Case-Control Studies ,030220 oncology & carcinogenesis ,Female ,business ,Biomarkers - Abstract
Purpose Gestational diabetes mellitus (GDM) is one of the most common medical problems of pregnancy. This study is designed to identify serum biomarkers, which can predict the subsequent development of GDM at early stages. Experimental design Maternal blood was obtained prospectively from pregnant women at 12-16 wk of pregnancy. Among these, 30 women were subsequently diagnosed with GDM at 24 to 28 wk and were selected as case studies along with 30 normoglycemic women as controls. Serum samples were analyzed by using iTRAQ analysis. Results Thirty three differentially expressed proteins were identified between case and control groups. They were involved in various signaling processes previously implied in GDM. Of which four proteins, i.e. apolipoprotein E, coagulation factor IX, fibrinogen alpha chain, and insulin-like growth factor-binding protein 5 were successfully verified by ELISA. Combinations of these four proteins, the area under the receiver operating characteristic curve, sensitivity, and specificity were 0.985, 80% and 95%, respectively. Conclusion The results highlight the roles of complement system, inflammatory and immune response, and blood coagulation in the pathogenesis of GDM. The panel of four candidate proteins could distinguish women subsequently developed with GDM from controls with high sensitivity and specificity.
- Published
- 2017
26. Sonographic evaluation of the development of the fetal rectum and anal canal
- Author
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Yi-Ming Su, Zhen Ye, Xin-Xiu Liu, Xiao-Yu Chen, and Shuqiang Chen
- Subjects
Adult ,Singleton pregnancy ,Rectum ,Anal Canal ,Gestational Age ,Ultrasonography, Prenatal ,Pregnancy ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Prospective Studies ,Fetus ,business.industry ,Ultrasound ,Gestational age ,Anatomy ,Nomogram ,Anal canal ,medicine.disease ,medicine.anatomical_structure ,Cross-Sectional Studies ,Regression Analysis ,Female ,business - Abstract
Purpose. To describe the sonographic (US) appearance of fetal anal canal and rectum and establish nomograms of their normal measurements. Methods. This was a prospective, cross-sectional study of 524 healthy women (mean age, 27 years; range, 21–37 years) with normal singleton pregnancy between 18 and 40 weeks of gestational age (GA). High-resolution transabdominal US was used to visualize and measure the normal fetal anal canal and rectum. Results. Satisfactory images and measurements of the fetal anal canal and rectum were obtained in 496 normal fetuses. The diameters of the normal anal canal and rectum were plotted as a function of GA in a sigmoid curve. The curve estimations were expressed by the following cubic regression equations with R2 of 0.87 and 0.88, respectively (p < 0.001): anal canal diameter (mm) = 18.272 − 2.151 × GA + 0.0095 × GA2 − 0.0011 × GA3, and rectal diameter (mm) = 18.545 − 2.543 × GA + 0.1237 × GA2 − 0.0016 × GA3. Conclusions. The fetal anal canal and rectum are visible sonographically between 18 and 40 weeks of GA. The knowledge of their normal US appearance and size from the second trimester of pregnancy onwards may help identify developmental anomalies. © 2011 Wiley Periodicals, Inc. J Clin Ultrasound, 2011
- Published
- 2010
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