1. Immunolocalization of dentin matrix protein-1 in human primary teeth treated with different pulp capping materials
- Author
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Natalino, Lourenço Neto, Nádia C T, Marques, Ana Paula, Fernandes, Camila O, Rodini, Vivien T, Sakai, Ruy Cesar C, Abdo, Maria Aparecida A M, Machado, Carlos F, Santos, and Thais M, Oliveira
- Subjects
Male ,Extracellular Matrix Proteins ,Humans ,Female ,Tooth, Deciduous ,Child ,Phosphoproteins ,Immunohistochemistry ,Pulp Capping and Pulpectomy Agents - Abstract
The aim of this study was to evaluate the immunolocalization of dentin matrix protein (DMP)-1 in human primary teeth treated with different pulp capping materials. Twenty-five primary molars were divided into the following groups: formocresol (FC), calcium hydroxide (CH), mineral trioxide aggregate (MTA), corticosteroid/antibiotic solution + CH (O + CH), and Portland cement (PC), and all received conventional pulpotomy treatment. The teeth at the regular exfoliation period were extracted for histological analysis and immunolocalization of DMP-1. Statistical analysis was performed using the χ(2) test (p 0.05). Histological analysis revealed statistically significant differences in the comparison among the groups through the use of a score system regarding the presence of hard tissue barrier, odontoblastic layer, and internal resorption, but not regarding pulp calcification. Immunohistochemical analysis showed immunostaining for DMP-1 in groups CH, MTA, O + CH, and PC. Internal resorption was observed in the groups FC and CH. MTA and PC showed pulp repair without inflammation and with the presence of hard tissue barrier. DMP-1 immunostaining was higher for MTA and PC, confirming the reparative and bioinductive capacity of these materials.
- Published
- 2014