1. Raman-guided subcellular pharmaco-metabolomics for metastatic melanoma cells.
- Author
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Du, Jiajun, Su, Yapeng, Qian, Chenxi, Yuan, Dan, Miao, Kun, Lee, Dongkwan, Ng, Alphonsus HC, Wijker, Reto S, Ribas, Antoni, Levine, Raphael D, Heath, James R, and Wei, Lu
- Subjects
Cell Line ,Tumor ,Humans ,Melanoma ,Stearoyl-CoA Desaturase ,Lipids ,Fatty Acids ,Oleic Acid ,Microscopy ,Spectrum Analysis ,Raman ,Apoptosis ,Lipid Metabolism ,Metabolomics ,Transcriptome ,Lipid Droplets ,Lipidomics ,Cell Line ,Tumor ,Spectrum Analysis ,Raman - Abstract
Non-invasively probing metabolites within single live cells is highly desired but challenging. Here we utilize Raman spectro-microscopy for spatial mapping of metabolites within single cells, with the specific goal of identifying druggable metabolic susceptibilities from a series of patient-derived melanoma cell lines. Each cell line represents a different characteristic level of cancer cell de-differentiation. First, with Raman spectroscopy, followed by stimulated Raman scattering (SRS) microscopy and transcriptomics analysis, we identify the fatty acid synthesis pathway as a druggable susceptibility for differentiated melanocytic cells. We then utilize hyperspectral-SRS imaging of intracellular lipid droplets to identify a previously unknown susceptibility of lipid mono-unsaturation within de-differentiated mesenchymal cells with innate resistance to BRAF inhibition. Drugging this target leads to cellular apoptosis accompanied by the formation of phase-separated intracellular membrane domains. The integration of subcellular Raman spectro-microscopy with lipidomics and transcriptomics suggests possible lipid regulatory mechanisms underlying this pharmacological treatment. Our method should provide a general approach in spatially-resolved single cell metabolomics studies.
- Published
- 2020