14 results on '"Xiaodan Wei"'
Search Results
2. Light-responsive hyaluronic acid nanomicelles co-loaded with an IDO inhibitor focus targeted photoimmunotherapy against 'immune cold' cancer
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Ningxi Li, Yiyao Liu, Zhengxin Xie, Shun Li, Honglin Huang, Xiaodan Wei, Hong Yang, Tingting Li, Jiming Xu, Chunhui Wu, and Xiang Qin
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Photosensitizing Agents ,Chemistry ,medicine.medical_treatment ,Biomedical Engineering ,Cancer ,Photoimmunotherapy ,Photodynamic therapy ,Dendritic cell ,medicine.disease ,Immune system ,Photochemotherapy ,Cell Line, Tumor ,Neoplasms ,Tumor Microenvironment ,Cancer research ,medicine ,Humans ,Immunogenic cell death ,Cytotoxic T cell ,General Materials Science ,Immunotherapy ,Hyaluronic Acid ,Checkpoint Blockade Immunotherapy - Abstract
Nanomedicine enabled cancer combination immunotherapy not only sufficiently activates the host immune system, but also reprograms the immunosuppressive microenvironment, representing a new generation approach to treat cancer. Herein, we demonstrated a targeted photo- and immune-active nanoplatform termed NLG919@HA-Ce6 to simultaneously elicit efficient immunogenic cell death (ICD) using the photosensitizer Ce6 and modulate the tryptophan metabolic pathway using an indoleamine 2,3-dioxygenase (IDO) inhibitor NLG919 for the combined photodynamic therapy (PDT) and checkpoint blockade immunotherapy. Against the triple-negative and poorly immunogenic 4T1 breast cancer model, the stable spherical nanomicelle NLG919@HA-Ce6 selectively killed tumour cells via the toxic singlet oxygen upon laser excitation, thus in situ triggering a potent antitumor immune response, as seen via the obvious CRT exposure, ATP release, dendritic cell maturation, etc. Meanwhile, the IDO1-mediated immunosuppression was effectively reprogrammed to an immunostimulatory phenotype, which was accompanied by an enhanced cytotoxic T cell response as well as reduced Treg infiltration in tumour bed. Ultimately, the 4T1 tumour was synergistically suppressed by NLG919@HA-Ce6 due to the outcome of focused PDT, obvious ICD post PDT and IDO1 blockade. This study suggests the promise of NLG919@HA-Ce6 as an alternative simple, stimulative and targeted nanoagent to enable the whole-body photo-immune therapy against "immune cold" cancer.
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- 2021
3. Multistage-responsive nanovehicle to improve tumor penetration for dual-modality imaging-guided photodynamic-immunotherapy
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Fengming You, Chunhui Wu, Tingting Li, Geng Yang, Xiaodan Wei, Shun Li, Xiaoxue Xie, Ningxi Li, Guixue Wang, Qingqing Su, Jie Zhu, Hanxi Zhang, Yiyao Liu, Chuan Zheng, Hong Yang, Yi Feng, and Xiang Qin
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medicine.medical_treatment ,Biophysics ,Bioengineering ,Photodynamic therapy ,02 engineering and technology ,Biomaterials ,03 medical and health sciences ,Cancer immunotherapy ,Cell Line, Tumor ,Neoplasms ,Tumor Microenvironment ,medicine ,Humans ,Cytotoxic T cell ,030304 developmental biology ,0303 health sciences ,Tumor microenvironment ,Chemistry ,X-Ray Microtomography ,Immunotherapy ,021001 nanoscience & nanotechnology ,Immune checkpoint ,Photochemotherapy ,Mechanics of Materials ,Drug delivery ,Ceramics and Composites ,Cancer research ,Matrix Metalloproteinase 2 ,Nanoparticles ,Nanocarriers ,0210 nano-technology - Abstract
The exploration of an intelligent multifunctional imaging-guided therapeutic platform is of great significance because of its ideal delivery efficiency and controlled release. In this work, a tumor microenvironment (TME)-responsive nanocarrier (denoted as MB@MSP) is designed for on-demand, sequentially release of a short D-peptide antagonist of programmed cell death-ligand 1 (named as PDPPA-1) and a photosensitizer methylene blue (MB). Fe3O4-Au located in the core of MB@MSP is used as a magnetic resonance imaging and micro-computed tomography imaging contrast agent for noninvasive diagnosis of solid tumors and simultaneous monitoring of drug delivery. The PDPPA-1 coated on MB@MSP can be shed due to the cleavage of the peptide substrate by matrix metalloproteinase-2 (MMP-2) that is highly expressed in the tumor stroma, and disulfide bonding is further broken when it encounters high levels of glutathione (GSH) in TME, which finally leads to significant size reduction and charge-reversal. These transitions facilitate penetration and uptake of nanocarriers against tumors. Noticeably, the released PDPPA-1 can block the immune checkpoint to create an environment that favors the activation of cytotoxic T lymphocytes and augment the antitumor immune response elicited by photodynamic therapy, thus significantly improving therapeutic outcomes. Studies of the underlying mechanisms suggest that the designed MMP-2 and GSH-sensitive delivery system not only induce apoptosis of tumor cells but also modulate the immunosuppressive tumor microenvironment to eventually augment the suppression tumor metastasis effect of CD8+ cytotoxic T cells. Overall, the visualization of the therapeutic processes with comprehensive information renders MB@MSP an intriguing platform to realize the combined treatment of metastatic tumors.
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- 2021
4. The impact of interleukin-10 (IL-10) gene 4 polymorphisms on peripheral blood IL-10 variation and prostate cancer risk based on published studies
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Xiaoying Qi, Jingjing Li, Fang Liu, Jia Mi, Tingting Men, Cuicui Yu, Xuri Li, Chunhua Yang, Dan Wang, Bin Wang, Geng Tian, Xiaodan Wei, and Wenguo Jiang
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Male ,Risk ,0301 basic medicine ,Oncology ,medicine.medical_specialty ,Genotype ,interleukin-10 ,Population ,Polymorphism, Single Nucleotide ,polymorphism ,03 medical and health sciences ,Prostate cancer ,0302 clinical medicine ,Gene Frequency ,Polymorphism (computer science) ,Internal medicine ,Odds Ratio ,medicine ,Humans ,Genetic Predisposition to Disease ,education ,Alleles ,education.field_of_study ,business.industry ,Prostatic Neoplasms ,Odds ratio ,Publication bias ,prostate cancer ,medicine.disease ,Confidence interval ,meta-analysis ,030104 developmental biology ,030220 oncology & carcinogenesis ,Meta-analysis ,peripheral blood interleukin-10 ,business ,Research Paper - Abstract
// Tingting Men 1, * , Cuicui Yu 2, * , Dan Wang 3 , Fang Liu 3 , Jingjing Li 3 , Xiaoying Qi 3 , Chunhua Yang 3 , Wenguo Jiang 3 , Xiaodan Wei 3 , Xuri Li 3 , Bin Wang 4 , Jia Mi 3 and Geng Tian 3 1 School of Nursing, Binzhou Medical University, Yantai, Shandong, China 2 Department of Anesthesiology, Yantai Yu Huang Ding Hospital, Yantai, Shandong, China 3 Medicine and Pharmacy Research Center, Binzhou Medical University, Yantai, Shandong, China 4 Institute of Molecular Imaging, Binzhou Medical University, Yantai, Shandong, China * These authors have contributed equally to this work Correspondence to: Geng Tian, email: tiangengshandong@yeah.net Jia Mi, email: Jia.mi@kemi.uu.se Bin Wang, email: binwang001@aliyun.com Keywords: prostate cancer, interleukin-10, polymorphism, peripheral blood interleukin-10, meta-analysis Received: December 16, 2016 Accepted: April 11, 2017 Published: April 29, 2017 ABSTRACT This study purported to investigate the impact of interleukin-10 (IL-10) gene 4 polymorphisms (-1082G>A, -819T>C, -592A>C and 210T>C) on peripheral blood IL-10 variation and prostate cancer (PCa) risk, with a special consideration given to various origins of between-study heterogeneity. 2 researchers independently fulfilled literature retrieval, quality assessment and information collection. Sub-grouped analyses per ethnicity, continent, design type, control source, genotyping procedure, genotype validation, age-matched status, study sample size, quality score and controls’ mean age were conducted, respectively. Total 17 unduplicated studies (patients/controls: 7561/8101) were assessable for PCa risk, and 4 unduplicated studies (1189 subjects) for peripheral blood IL-10 variation. Pooling all assessable studies identified a marginally significant association between the -1082A allele and increased PCa risk (odds ratio (OR)=1.10, 95% confidence interval [CI]: 1.00 to 1.21) (Heterogeneity I 2 =64.3%), and no significance was detected in sub-grouped analyses of this polymorphism. Contrastingly, the -592C allele was significantly associated with reduced PCa risk in both prospective (OR=0.85, 95% CI: 0.77 to 0.95) and population-based (OR=0.92, 95% CI: 0.84 to 1.00) studies (Heterogeneity I 2 =0.0% and 18.1%). Moreover, carriers of combined -592CA/CC genotypes had a significant higher level of peripheral blood IL-10 than the -592AA genotype carriers (weighted mean difference=0.45 and 0.54 mg/dL, 95% CI: 0.23 to 0.67 and 0.30 to 0.39). The above comparisons possessed a low probability of publication bias. In sum, our findings suggested that IL-10 gene -592A>C polymorphism may represent a promising candidate locus for the occurrence of PCa, and further signified a contributing role of this polymorphism in prostate carcinogenesis.
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- 2017
5. Quantitative proteomic analysis of gastric cancer tissue reveals novel proteins in platelet-derived growth factor B signaling pathway
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Geng Tian, Jia Mi, Jonas Bergquist, Bin Wang, Tingting Men, Chunhua Yang, Xiaodan Wei, Fang Liu, Xingyue Jiang, Dong Yan, He Li, Gangming Feng, Wenguo Jiang, Yuan Zhang, and Jianke Yang
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Proteomics ,0301 basic medicine ,Proteome ,Cathepsin D ,Apoptosis ,PRDX5 ,Receptor, Platelet-Derived Growth Factor beta ,03 medical and health sciences ,0302 clinical medicine ,Stomach Neoplasms ,Biomarkers, Tumor ,Tumor Cells, Cultured ,Humans ,Medicine ,Gene silencing ,Protein Interaction Maps ,Phosphorylation ,Protein kinase B ,proteomic ,Cell Proliferation ,biology ,Traditional medicine ,pathway ,business.industry ,gastric cancer ,Cancer ,Proto-Oncogene Proteins c-sis ,PDGF-B ,medicine.disease ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,biology.protein ,business ,Proto-Oncogene Proteins c-akt ,Calreticulin ,Platelet-derived growth factor receptor ,Research Paper ,Signal Transduction - Abstract
// Fang Liu 1, 2, * , Yuan Zhang 1, * , Tingting Men 1 , Xingyue Jiang 2 , Chunhua Yang 1 , He Li 4 , Xiaodan Wei 1 , Dong Yan 1 , Gangming Feng 5 , Jianke Yang 1 , Jonas Bergquist 3 , Bin Wang 2 , Wenguo Jiang 1 , Jia Mi 1, 3 , Geng Tian 1 1 Medicine and Pharmacy Research Center, Binzhou Medical University, Yantai, Shandong Province, 264003 China 2 Department of Radiology, Affiliated Hospital of Binzhou Medical University, Binzhou, Shandong Province, 256603 China 3 Department of Chemistry – BMC, Uppsala University, Uppsala, 75124, Sweden 4 Department of Gastric and Intestine, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong Province, 264003 China 5 Yantai Institute, China Agriculture University, Yantai, Shandong Province, 264670 China * Shared first author Correspondence to: Geng Tian, email: Tiangeng@live.se Wenguo Jiang, email: jiangwg@foxmail.com Jia Mi, email: jia.mi@kemi.uu.se Keywords: proteomic, PDGF-B, gastric cancer, PRDX5, pathway Received: December 02, 2016 Accepted: February 07, 2017 Published: March 06, 2017 ABSTRACT Gastric cancer is one of the most common cancers in Asian countries. Searching for reliable biomarkers involving the development of gastric cancer is important for clinical practice. Quantitative proteomics has become an important method contributed to the discovery of novel diagnostic or therapeutic targets for the management of cancer. Here, we identified differently expressed proteins in gastric cancer and normal gastric tissues by using the high resolution mass spectrometer. Among the total of 2280 identified proteins, 87 were differentially expressed between gastric cancer and normal gastric tissues. Notably, several significant proteins are in the PDGF-B signaling pathway, including peroxiredoxin5 (PRDX5), S100A6, calreticulin (CALR) and cathepsin D (CTSD), which were validated by western blot. Furthermore, upstream regulators including PDGF-B, PDGFR-β, Akt, eIF4E and p70s6K were found significantly increased in the gastric cancer tissues. In addition, silencing of PRDX5 and PDGF-B suppressed the proliferation of gastric cancer cells in vitro . The administration of exogenous PDGF-BB recovered the reduced expression of PDGF-B signaling pathway in PDGF-B knockdown cells. Taken together, our findings suggested that PDGF-B signaling pathway plays an important role in the regulation of gastric cancer proliferation and the inhibition of this pathway may be a potential approach for treatment of gastric cancer.
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- 2017
6. A systematic review and meta-analysis: Does hepatitis C virus infection predispose to the development of chronic kidney disease?
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Geng Tian, Min Li, Xiaodan Wei, Xuri Li, Jia Mi, Chunhua Yang, Peiyuan Wang, Wenguo Jiang, and Xinbo Mu
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hepatitis C virus ,medicine.medical_specialty ,Time Factors ,Hepatitis C virus ,030232 urology & nephrology ,Renal function ,Review ,Kidney ,medicine.disease_cause ,Risk Assessment ,Severity of Illness Index ,effect estimate ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Internal medicine ,Odds Ratio ,Prevalence ,medicine ,Humans ,Renal Insufficiency, Chronic ,Proteinuria ,business.industry ,Incidence ,Hazard ratio ,Odds ratio ,Prognosis ,medicine.disease ,Hepatitis C ,Confidence interval ,meta-analysis ,Oncology ,Meta-analysis ,030211 gastroenterology & hepatology ,medicine.symptom ,business ,chronic kidney disease ,Glomerular Filtration Rate ,Kidney disease - Abstract
// Min Li 1,* , Peiyuan Wang 2,* , Chunhua Yang 1 , Wenguo Jiang 1 , Xiaodan Wei 1 , Xinbo Mu 3 , Xuri Li 1 , Jia Mi 1 and Geng Tian 1 1 Medicine and Pharmacy Research Center, Binzhou Medical University, Yantai, Shandong, China 2 Institute of Imaging, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong, China 3 Personnel Department, Binzhou Medical University, Yantai, Shandong, China * These authors have contributed equally to this work Correspondence to: Geng Tian, email: // Jia Mi, email: // Xuri Li, email: // Keywords : hepatitis C virus; chronic kidney disease; meta-analysis; effect estimate Received : August 19, 2016 Accepted : October 14, 2016 Published : October 25, 2016 Abstract We aimed to meta-analytically assess the predisposition of hepatitis C virus (HCV) infection to the occurrence and severity of chronic kidney disease (CKD). Two authors independently searched articles and abstracted information. Odds ratio (OR) or hazard ratio (HR) along with 95% confidence interval (CI) was converged separately in 12 longitudinal (1,972,044 subjects) and 15 cross-sectional (937,607 subjects) studies. Overall effect estimate was remarkably significant in longitudinal studies (HR, 95% CI, P : 1.45, 1.23-1.71, 95%). HCV infection was also associated with an 1.54-fold (95% CI, P : 1.27-1.87, < 0.001) increased risk of having prevalent proteinuria. In longitudinal studies with estimated glomerular filtration rate (eGFR) < 60, < 30 and < 15 ml/min/1.73m 2 , the corresponding HR was 1.39 (95% CI, P : 1.14-1.69, 0.001), 1.79 (0.91-3.51, 0.091) and 2.30 (1.26-4.19, 0.007). Further grouping the longitudinal studies by median follow-up time at 5 years revealed that the effect estimate was reinforced in long-term studies (HR, 95% CI, P : 1.86, 1.19-2.89, 0.006; I 2 =98.1%) relative to that in short-term studies (1.21, 1.03-1.43, 0.024; 92.0%). In conclusion, our findings demonstrate the significant risk of experiencing incident CKD after HCV infection, with the lower eGFR and longer HCV exposure time entailing a greater risk.
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- 2016
7. Imbalanced Randomization in Clinical Trials
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Rick Chappell, Xiaodan Wei, and Thevaa Chandereng
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Statistics and Probability ,FOS: Computer and information sciences ,Randomization ,Epidemiology ,business.industry ,Confounding ,Variance (accounting) ,01 natural sciences ,Statistics - Applications ,Treatment and control groups ,Clinical trial ,010104 statistics & probability ,03 medical and health sciences ,Patient population ,Random Allocation ,0302 clinical medicine ,Bias ,Margin (machine learning) ,Statistics ,Medicine ,Humans ,Applications (stat.AP) ,030212 general & internal medicine ,0101 mathematics ,business - Abstract
Randomization is a common technique used in clinical trials to eliminate potential bias and confounders in a patient population. Equal allocation to treatment groups is the standard due to its optimal efficiency in many cases. However, in certain scenarios, unequal allocation can improve efficiency. In superiority trials with more than two groups, the optimal randomization is not always a balanced randomization. In non-inferiority trials, additive margin with equal variance is the only instance with balanced randomization. Optimal randomization for non-inferiority trials can be far from 1:1 and can greatly improve efficiency, a fact which is commonly overlooked. A tool for sample size calculation for non-inferiority trials with additive or multiplicative margin with normal, binomial or Poisson distribution is available at http://www.statlab.wisc.edu/shiny/SSNI/.
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- 2018
8. Association of APE1 Gene Asp148Glu Variant with Digestive Cancer: A Meta-Analysis
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Shuping Zhang, Xiaodan Wei, He Li, Dongmei Zhao, Jia Mi, Jing Zou, and Geng Tian
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chemistry.chemical_classification ,Genetics ,Aspartic Acid ,Genetic heterogeneity ,Glutamic Acid ,General Medicine ,Biology ,Digestive System Neoplasms ,medicine.disease_cause ,DNA-(apurinic or apyrimidinic site) lyase ,Genetic Heterogeneity ,Enzyme ,chemistry ,Meta-analysis ,DNA-(Apurinic or Apyrimidinic Site) Lyase ,medicine ,Humans ,AP site ,Carcinogenesis ,Gene ,Digestive cancer ,Genetic Association Studies ,Meta-Analysis - Abstract
Background Apurinic/apyrimidinic endonuclease-1 (APE1) is a rate-limiting enzyme in DNA base excision repair and has been implicated in carcinogenesis. In this study, we summarize available data to examine the susceptibility of APE1 gene Asp148Glu variant to digestive cancer via a meta-analysis. Material/Methods Study selection and data abstraction were conducted independently by 2 authors. Random-effects model was utilized to pool effect estimates. Heterogeneity and publication bias were addressed. Results Sixteen articles involving 4916 digestive cancer patients and 7748 controls were qualified for this meta-analysis. Overall association showed an indicative association between Asp148Glu variant and digestive cancer under allelic (odds ratio or OR=1.11; 95% confidence interval or CI: 0.99–1.25; P=0.074) and dominant (OR=1.18; 95% CI: 1.00–1.40; P=0.056) models, with strong evidence of heterogeneity. Deviation from Hardy-Weinberg equilibrium was an obvious source of heterogeneity. In subgroup analyses by cancer sites, this variant was significantly associated with the increased risk for hepatocellular cancer under allelic (OR=1.50; 95% CI: 1.25–1.80; P
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- 2015
9. A multicenter matched case-control analysis on seven polymorphisms from HMGB1 and RAGE genes in predicting hepatocellular carcinoma risk
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Chuanhua Yang, Wenguo Jiang, Geng Tian, Xuri Li, Xiaodan Wei, Xiaoying Qi, Dan Wang, Jia Mi, and Fang Liu
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0301 basic medicine ,Male ,medicine.medical_specialty ,haplotype ,Carcinoma, Hepatocellular ,Receptor for Advanced Glycation End Products ,Gastroenterology ,Polymorphism, Single Nucleotide ,HMGB1/RAGE axis ,polymorphism ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Internal medicine ,Genotype ,Genetic predisposition ,Medicine ,Humans ,Genetic Predisposition to Disease ,Allele ,HMGB1 Protein ,Polymorphism, Genetic ,business.industry ,Haplotype ,Liver Neoplasms ,Odds ratio ,hepatocellular carcinoma ,Middle Aged ,medicine.disease ,Confidence interval ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,Case-Control Studies ,Immunology ,Female ,Clinical Research Paper ,business ,Body mass index ,genetic susceptibility - Abstract
// Dan Wang 1,* , Xiaoying Qi 1 , Fang Liu 1,* , Chuanhua Yang 1 , Wenguo Jiang 1 , Xiaodan Wei 1 , Xuri Li 1 , Jia Mi 1 and Geng Tian 1 1 Medicine and Pharmacy Research Center, Binzhou Medical University, Yantai, Shandong, China * The first two authors have contributed equally to this work Correspondence to: Geng Tian, email: // Jia Mi, email: // Xuri Li, email: // Keywords : hepatocellular carcinoma; HMGB1/RAGE axis; polymorphism; genetic susceptibility; haplotype Received : December 22, 2016 Accepted : January 25, 2017 Published : February 08, 2017 Abstract Based on 540 hepatocellular carcinoma patients and 540 age- and gender-matched controls, we tested the hypothesis that high mobility group protein box1 ( HMGB1 ) and the receptor for advanced glycation end products ( RAGE ) genes are two potential candidate susceptibility genes for hepatocellular carcinoma in a multicenter hospital-based case-control analysis. The genotypes of seven widely-studied polymorphisms were determined, and their distributions respected the Hardy-Weinberg equilibrium. The mutant alleles of two polymorphisms, rs1045411 in HMGB1 gene and rs2070600 in RAGE gene, had significantly higher frequencies in patients than in controls ( P < 0.001), with the power to detect this significance of being over 99.9%. Moreover, the above two polymorphisms increased the risk of developing hepatocellular carcinoma significantly, particularly for rs2070600 under the additive (odds ratio [OR] = 1.77; 95% confidence interval [CI]: 1.34-2.32; P < 0.001) and dominant (OR = 1.75; 95% CI: 1.23-2.50; P = 0.002) models after adjusting for body mass index, smoking and drinking. Haplotype analysis showed that the T-C-T haplotype (rs1045411-rs2249825-rs1415125) in HMGB1 gene was associated with a 2.47-fold (95% CI: 1.41-4.34; P = 0.002) increased risk of hepatocellular carcinoma compared with the commonest C-C-T haplotype after adjustment. In RAGE gene, the T-T-A-G (rs1800625-rs1800624-rs2070600-rs184003) (adjusted OR; 95% CI; P: 1.75; 1.02-3.03; 0.045) and T-T-A-T (adjusted OR; 95% CI; P: 1.95; 1.01-3.76; 0.048) haplotypes were associated with a marginally increased risk of hepatocellular carcinoma compared with the commonest T-T-G-G haplotype. In summary, we identified two risk-associated polymorphisms (rs1045411 and rs2070600), and more importantly a joint impact of seven polymorphisms from the HMGB1/RAGE axis in susceptibility to hepatocellular carcinoma.
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- 2016
10. Circulating interleukin-6 and cancer: A meta-analysis using Mendelian randomization
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Bin Wang, Xuri Li, Dongmei Zhao, Lingyan Qiao, Geng Tian, Jia Mi, Chunhua Yang, Shuping Zhang, Xiaodan Wei, and Xianglin Li
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Oncology ,medicine.medical_specialty ,Multidisciplinary ,Genotype ,Interleukin-6 ,Liver Neoplasms ,Cancer ,Mendelian Randomization Analysis ,Odds ratio ,Biology ,Lower risk ,medicine.disease ,Bioinformatics ,Polymorphism, Single Nucleotide ,Article ,Random Allocation ,Asian People ,Internal medicine ,Mendelian randomization ,medicine ,Humans ,Genetic Predisposition to Disease ,Allele ,Liver cancer - Abstract
Interleukin-6 (IL-6) plays a contributory role in the progression and severity of many forms of cancer; it however remains unclear whether the relevance between circulating IL-6 and cancer is causal. We therefore meta-analyzed published articles in this regard using IL-6 gene -174G/C variant as an instrument. Seventy-eight and six articles were eligible for the association of -174G/C variant with cancer and circulating IL-6, respectively. Overall analyses failed to identify any significance between -174G/C and cancer risk. In Asians, carriers of the -174CC genotype had an 1.95-fold increased cancer risk compared with the -174GG genotype carriers (P = 0.009). By cancer type, significance was only attained for liver cancer with the -174C allele conferring a reduced risk under allelic (odds ratio or OR = 0.74; P = 0.001), homozygous genotypic (OR = 0.59; P = 0.029) and dominant (OR = 0.67; P = 0.004) models. Carriers of the -174CC genotype (weighted mean difference or WMD = −4.23 pg/mL; P
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- 2015
11. Causal relevance of circulating high-density lipoprotein cholesterol with cancer: a Mendelian randomization meta-analysis
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Xuri Li, Jia Mi, Chunhua Yang, Wenming Wang, Xiaodan Wei, Xianglin Li, Geng Tian, and Bin Wang
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Oncology ,Apolipoprotein E ,Risk ,medicine.medical_specialty ,Genotype ,Biology ,Bioinformatics ,Article ,Apolipoproteins E ,Polymorphism (computer science) ,Internal medicine ,Neoplasms ,Mendelian randomization ,medicine ,Humans ,Allele ,Risk factor ,Alleles ,Multidisciplinary ,Polymorphism, Genetic ,Cholesterol, HDL ,Cancer ,Odds ratio ,medicine.disease ,Lipids - Abstract
We summarized published data on the associations of apolipoprotein E (APOE) gene ε2/ε3/ε4 polymorphism with both cancer risk and circulating lipid profiles, aiming to examine the causal relevance between lipids and cancer risk. Article identification and data abstraction were conducted in duplicate and independently by two authors. Data were analyzed by STATA software. Twenty-five articles that examined the associations of APOE gene ε2/ε3/ε4 polymorphism with either cancer risk (n = 22) or circulating lipid changes (n = 4) were eligible. The presence of ε2 and ε4 alleles showed no overall associations with overall cancer risk when compared with ε3 allele. The ε4 allele was significantly associated with 1.40-fold (odds ratio or OR = 1.40; 95% confidence interval or CI: 1.00–1.94; P = 0.047) increased risk of developing cancer in Asian populations and the presence of heterogeneity was low (I2 = 37.6%). Carriers of ε3/ε4 genotype had a significant reduction in circulating HDL-C (WMD = −2.62; 95% CI: −4.19 to −1.04; P = 0.001) without heterogeneity (I2 = 16.6%). The predicted odds of having cancer for 1 mg/dL reduction in circulating HDL-C was 1.14 (95% CI: 1.00 to 1.89). The findings of this Mendelian randomization meta-analysis demonstrate that reduced circulating HDL-C might be a potentially causal risk factor for the development of overall cancer in Asians.
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- 2015
12. The Number of Stenotic Intracranial Arteries Is Independently Associated with Ischemic Stroke Severity
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Min Li, Geng Tian, Xianglin Li, Xuri Li, Shuping Zhang, Wenming Wang, Bin Wang, Zhuang Liu, Jonas Bergquist, Chunhua Yang, Jia Mi, and Xiaodan Wei
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Male ,Carotid arteries ,lcsh:Medicine ,Constriction, Pathologic ,Cardiovascular Medicine ,030204 cardiovascular system & hematology ,Pathology and Laboratory Medicine ,Vascular Medicine ,Biochemistry ,Severity of Illness Index ,Brain Ischemia ,0302 clinical medicine ,Risk Factors ,Medicine and Health Sciences ,Cardiac and Cardiovascular Systems ,lcsh:Science ,Stroke ,Stenosis ,Kardiologi ,Multidisciplinary ,Arteries ,Middle Aged ,Lipids ,Cholesterol ,Carotid Arteries ,Neurology ,Cardiovascular Diseases ,Cardiology ,Female ,Anatomy ,Research Article ,Adult ,medicine.medical_specialty ,Cerebrovascular Diseases ,Lipoproteins ,macromolecular substances ,Young Adult ,03 medical and health sciences ,Signs and Symptoms ,Diagnostic Medicine ,Internal medicine ,medicine ,Humans ,cardiovascular diseases ,Ischemic Stroke ,Aged ,Retrospective Studies ,business.industry ,lcsh:R ,Biology and Life Sciences ,Proteins ,Angiography, Digital Subtraction ,medicine.disease ,Surgery ,Ischemic stroke ,Cardiovascular Anatomy ,Blood Vessels ,lcsh:Q ,Intracranial Arterial Diseases ,business ,030217 neurology & neurosurgery - Abstract
Background The severity of ischemic stroke symptoms varies among patients and is a critical determinant of patient outcome. To date, the association between the number of stenotic intracranial arteries and stroke severity remains unclear. Aims We aimed to investigate the association between the number of stenotic major intracranial arteries (NSMIA) and ischemic stroke severity, as well as the degree of stenosis and common stroke risk factors. Methods We performed a retrospective analysis of patients with digital subtraction angiography (DSA)-confirmed ischemic stroke. Clinical stroke severity was measured using the National Institutes of Health Stroke Scale (NIHSS). The number of stenotic vessels was counted from the internal carotid arteries and vertebral arteries, bilaterally. Results Eighty three patients were recruited from a single center and included in the study. NSMIA was significantly correlated with stroke severity (Pearson Correlation Coefficient = 0.485, P < 0.001), but not with the degree of stenosis (Pearson Correlation Coefficient = 0.01, P = 0.90). Multivariate regression analysis revealed that NSMIA was significantly associated with the NIHSS score after adjusting for stroke risk factors. The adjusted odds ratio (per lateral) was 2.092 (95% CI, 0.865 to 3.308, P = 0.001). The degree of stenosis was also significantly associated with the NIHSS score after adjusting for common risk factors. The odds ratio (per 10%) was 0.712 (95% CI, 0.202 to 1.223, P = 0.007). Conclusions The number of stenotic intracranial major arteries is associated with the severity of ischemic stroke independent of the degree of stenosis and other stroke risk factors. To the best of our knowledge, this has not been previosuly studied in great detail using DSA. Our data highlight the importance of examining all major arteries in stroke patients.
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- 2016
13. Genetic analysis of the promoter region of the GATA4 gene in patients with ventricular septal defects
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Hao Zhang, Jiping Shan, Bo Yan, Xiaodan Wei, Shuchao Pang, and Guanghua Wu
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Adult ,Heart Septal Defects, Ventricular ,Male ,Heterozygote ,Adolescent ,Biology ,Genetic analysis ,Polymerase Chain Reaction ,Young Adult ,Physiology (medical) ,Gene expression ,Coding region ,Humans ,Child ,Promoter Regions, Genetic ,Gene ,Transcription factor ,DNA Primers ,Genetics ,Base Sequence ,GATA4 ,Biochemistry (medical) ,Public Health, Environmental and Occupational Health ,Infant ,Promoter ,General Medicine ,Molecular biology ,GATA4 Transcription Factor ,Case-Control Studies ,Child, Preschool ,GATA transcription factor ,Female - Abstract
Ventricular septal defects (VSDs) are the most common type of congenital heart diseases (CHDs). To date, the genetic causes for sporadic VSDs remain largely unknown. GATA transcription factor 4 (GATA4) is a zinc-finger transcription factor that is expressed in developing heart and adult cardiomyocytes. Mutations in the coding region of the GATA4 gene have been identified in CHD patients, including VSD. As the GATA4 factor is a dosage-sensitive regulator, we hypothesized that the promoter region variants of the GATA4 gene may be genetic causes of VSD. In this study, we analyzed the promoter region of the GATA4 gene by bidirectional sequencing in 172 VSD patients and 171 healthy controls. The results showed that 5 heterozygous sequence variants (NG_008177:g.4071T>C, NG_008177:g.4148C>A, NG_008177:g.4566C>T, NG_008177:g.4653G>T, and NG_008177:g.4690G>deletion) within the promoter region of the GATA gene were identified in 5 VSD patients, but in none of controls. One heterozygous sequence variant (g.4762C>A) was found only in one control, which may have no functional significance. A functional analysis revealed that the transcriptional activity of variant NG_008177:g.4566C>T was reduced significantly, whereas the transcriptional activities of the variants (NG_008177:g.4071T>C, NG_008177:g.4148C>A, NG_008177:g.4653G>T, and NG_008177:g.4690G>deletion) were increased significantly compared with the wild-type GATA4 gene promoter. As GATA4 is a dosage-sensitive regulator during development, our data suggest that these sequence variants within the promoter region of the GATA4 gene may contribute to the VSD etiology by altering its gene expression. Additional studies in experimental animals will deepen our understanding of the genetic basis of VSD and shed light on designing novel molecular therapies for adult VSD patients carrying these variants.
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- 2011
14. Calcium supplement ingestion may alter lumbar spine bone mineral density measurement
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Diane Krueger, Mary Checovich, Dessa Gemar, Neil Binkley, and Xiaodan Wei
- Subjects
musculoskeletal diseases ,Male ,Time Factors ,Endocrinology, Diabetes and Metabolism ,Elemental calcium ,chemistry.chemical_element ,Calcium ,Calcium supplement ,law.invention ,Calcium Carbonate ,Absorptiometry, Photon ,Randomized controlled trial ,law ,Bone Density ,Medicine ,Ingestion ,Humans ,Radiology, Nuclear Medicine and imaging ,Orthopedics and Sports Medicine ,Aged ,Bone mineral ,Aged, 80 and over ,Lumbar Vertebrae ,business.industry ,Middle Aged ,musculoskeletal system ,chemistry ,Dietary Supplements ,Lumbar spine ,Female ,business ,Nuclear medicine ,Densitometry ,Tablets - Abstract
Densitometry centers commonly request patients abstain from ingesting calcium supplements prior to dual-energy X-ray absorptiometry (DXA) examination to avoid interference with bone mineral density (BMD) measurement. However, it is not clear that this practice is necessary. This study assessed the impact of recent calcium supplement intake on lumbar spine BMD measurement. The phase 1 experiment demonstrated BMD differences when placing a supplement over various areas of two encapsulated phantoms. To determine whether these results were clinically relevant, the phase 2 study enrolled 36 subjects who received spine scans before and after random assignment to ingest one Citracal, OsCal or People's Choice tablet providing 315, 500, and 600 mg of elemental calcium, respectively. The phase 3 study evaluated 15 subjects that had three spine scans with repositioning between to establish BMD precision, and then ingested a calcium carbonate tablet. Post-dosing scans were performed 15 and 30 minutes after tablet ingestion. The difference in L1-L4 BMD on subsequent scans was compared with the study derived least significant change (LSC) of 0.028 g/cm(2). In phase 2, L1-L4 BMD changes outside the LSC occurred in 36% of subjects with 47% having visualized tablets. Similarly, 33% of the phase 3 subjects demonstrated changes outside the LSC after calcium ingestion and 53% of the subjects in phase 3 had tablets visualized. Visualization of calcium tablets was not associated with BMD changes. In conclusion, calcium tablet intake may affect measured L1-L4 BMD within 30 minutes of ingestion. As such, it is appropriate to request patients abstain from calcium tablet ingestion prior to a spine DXA examination.
- Published
- 2005
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