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Your search keyword '"HYALURONAN-BINDING PROTEINS"' showing total 4 results
Start Over Descriptor "HYALURONAN-BINDING PROTEINS" Topic hyaluronic acid
4 results on '"HYALURONAN-BINDING PROTEINS"'

1. Determination of Cell-Surface Hyaluronan Through Flow Cytometry.

Author

Vitale DL, Spinelli FM, and Alaniz L

Subjects
Animals, Cell Line, Fluorescent Dyes analysis, Humans, Staining and Labeling methods, Cell Membrane chemistry, Flow Cytometry methods, Hyaluronic Acid analysis
Abstract

Hyaluronan is the major glycosaminoglycan present in the extracellular matrix of several cell types; its synthesis occurs on the cellular plasma membrane. Variations in its expression are related to alterations in cell proliferation, adhesion, and migration. It is able to interact with different binding proteins called hyaladherins, which can be conjugated to different fluorochromes and analyzed by flow cytometry.

Published
2019
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2. Hyaluronan-binding receptors: possible involvement in osteoarthritis.

Author

Dunn, Sharon, Kolomytkin, Oleg V., Waddell, David D., and Marino, Andrew A.

Subjects
*HYALURONIC acid, *MUCOPOLYSACCHARIDES, *ORGANIC acids, *OSTEOARTHRITIS, *ARTHRITIS
Abstract

Our objectives were to compare the expression of the hyaluronan receptors CD44 and RHAMM in knee synovial tissue of patients with and without advanced osteoarthritis (OA). Both receptors were detected immunohistochemically; the staining appeared more intense in the tissues from the patients with advanced OA. Expression of CD44 and RHAMM were each significantly increased ( p < 0.05) in synovial tissue from patients with OA, as determined by means of Western-blot analysis. The findings suggested that changes in levels of the HA-binding proteins might be implicated in the development or progression of OA. [ABSTRACT FROM AUTHOR]

Published
2009
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3. Visualisation of hyaluronan and hyaluronan-binding proteins within ovine vertebral cartilages using biotinylated aggrecan G1-link complex and biotinylated hyaluronan oligosaccharides.

Author

Melrose, J., Tammi, M., and Smith, S.

Subjects
HYALURONIC acid, MUCOPOLYSACCHARIDES, ORGANIC acids, BIOTIN, ALBUMINS, CHROMATOGRAPHIC analysis
Abstract

The aim of this study was to localise hyaluronan (HA)-binding proteins (HABPs) in ovine vertebral tissues using biotinylated HA oligosaccharides (bHA oligos) as novel affinity probes and to compare this with the distribution of tissue HA visualised using biotinylated aggrecan G1 domain–link protein complex. The bHA oligos, with a size of 6–18 disaccharides were prepared by partial digestion of HA with ovine testicular hyaluronidase, labelled with biotin hydrazide and purified by a combination of aggrecan G1 domain and avidin affinity chromatography. Hyaluronan and HABPs were both prominent pericellular components of hypertrophic cells of the vertebral epiphyseal growth plate and enlarged cells in the cartilaginous end plate of the disc. The bHA oligo probe also visualised HABPs intracellularly in hypertrophic cells, which also contained intracellular HA. Monolayer cultures of ovine annulus fibrosus and nucleus pulposus cells rapidly internalised the bHA oligo affinity probe which was subsequently visualised by indirect fluorescence using avidin-FITC, to cytoplasm and discrete nuclear regions. The results indicate that the abundant pericellular and intracellular HA associated with cartilaginous cells in the vertebral tissues is colocalised with HABPs. The bHA oligo affinity probe may have further applications in investigations of intracellular HABPs, HA endocytosis and the roles they play in cellular regulatory processes. [ABSTRACT FROM AUTHOR]

Published
2002
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4. Aggravation of Allergic Airway Inflammation by Cigarette Smoke in Mice Is CD44-Dependent

Author

Sharen Provoost, Katrien De Grove, Ken R. Bracke, Ellen A. Lanckacker, Guy Brusselle, Mieke A. Dentener, Emiel F.M. Wouters, Smitha Kumar, Guy Joos, Tania Maes, RS: NUTRIM - R3 - Chronic inflammatory disease and wasting, Pulmonologie, and MUMC+: MA Longziekten (3)

Subjects
Male, INDUCED MURINE MODEL, 0301 basic medicine, Allergy, Pulmonology, Exacerbation, Physiology, lcsh:Medicine, CD8-Positive T-Lymphocytes, Pathology and Laboratory Medicine, ACTIVATION, White Blood Cells, Habits, Animal Cells, Immune Physiology, Smoking Habits, Medicine and Health Sciences, Osteopontin, Hyaluronic Acid, Receptor, lcsh:Science, Immune Response, Lung, Sensitization, Mice, Knockout, Innate Immune System, Multidisciplinary, biology, Pyroglyphidae, Smoking, Animal Models, respiratory system, SENSITIZATION, Hyaluronan Receptors, medicine.anatomical_structure, Cytokines, Goblet Cells, Anatomy, Cellular Types, medicine.symptom, Bronchoalveolar Lavage Fluid, Research Article, EXPRESSION, Immune Cells, Immunology, Antigen-Presenting Cells, Mouse Models, Inflammation, Research and Analysis Methods, DENDRITIC CELLS, CD44 ISOFORMS, Lymphatic System, 03 medical and health sciences, Signs and Symptoms, Model Organisms, Th2 Cells, Diagnostic Medicine, Hypersensitivity, medicine, Animals, Asthma, Behavior, Metaplasia, Blood Cells, business.industry, CD44, lcsh:R, LUNG INFLAMMATION, Biology and Life Sciences, Cell Biology, Dendritic Cells, Pneumonia, Molecular Development, medicine.disease, respiratory tract diseases, Eosinophils, Mice, Inbred C57BL, 030104 developmental biology, Immune System, biology.protein, Th17 Cells, ASTHMA, lcsh:Q, Lymph Nodes, business, HYALURONAN-BINDING PROTEINS, Developmental Biology, RESPONSES
Abstract

Background : Although epidemiological studies reveal that cigarette smoke (CS) facilitates the development and exacerbation of allergic asthma, these studies offer limited information on the mechanisms involved. The transmembrane glycoprotein CD44 is involved in cell adhesion and acts as a receptor for hyaluronic acid and osteopontin. We aimed to investigate the role of CD44 in a murine model of CS-facilitated allergic airway inflammation. Methods : Wild type (WT) and CD44 knock-out (KO) mice were exposed simultaneously to house dust mite (HDM) extract and CS. Inflammatory cells, hyaluronic acid (HA) and osteopontin (OPN) levels were measured in bronchoalveolar lavage fluid (BALF). Proinflammatory mediators, goblet cell metaplasia and peribronchial eosinophilia were assessed in lung tissue. T-helper (Th) 1, Th2 and Th17 cytokine production was evaluated in mediastinal lymph node cultures. Results : In WT mice, combined HDM/CS exposure increased the number of inflammatory cells and the levels of HA and OPN in BALF and Th2 cytokine production in mediastinal lymph nodes compared to control groups exposed to phosphate buffered saline (PBS)/CS, HDM/Air or PBS/Air. Furthermore, HDM/CS exposure significantly increased goblet cell metaplasia, peribronchial eosinophilia and inflammatory mediators in the lung. CD44 KO mice exposed to HDM/CS had significantly fewer inflammatory cells in BALF, an attenuated Th2 cytokine production, as well as decreased goblet cells and peribronchial eosinophils compared to WT mice. In contrast, the levels of inflammatory mediators were similar or higher than in WT mice. Conclusion : We demonstrate for the first time that the aggravation of pulmonary inflammation upon combined exposure to allergen and an environmental pollutant is CD44-dependent. Data from this murine model of concomitant exposure to CS and HDM might be of importance for smoking allergic asthmatics.

Published
2016

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