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Abstract??Nocardiasp. 108 exhibited strong acrylonitrile-hydrating activity and its nitrile hydratase was Co2+-dependent.Nocardiasp. 108 was active within a broad pH range from 6.0 to 10.0 at 30?C and thermostable at temperatures below 35?C, but became unstable at temperatures above 45?C. Furthermore, it was found thatNocardiasp. 108 can hydrate indole-3-acetonitrile,p-chlorobenzonitrile,p-hydroxybenzylcyanide, 3,4,5-trimethoxybenzonitrile,p-aminobenzonitrile, 3-cyanopyridine,o-chlorobenzonitrile to the corresponding amides and hence displayed a broad substrate specificity. The temperature and pH optima for these hydrations were 28?C and pH 7.0?7.5, respectively. At the observed concentrations, acrylonitrile was completely converted within 5?min, while 3,4,5-trimethoxybenzonitrile,p-aminobenzonitrile, indole-3-acetonitrile,p-chlorobenzonitrile were approximately 21.71, 8.98, 34.44, 93.10% hydrated.p-Chlorobenzonitrile appeared to be the preferred aromatic nitrile forNocardiasp. 108. [ABSTRACT FROM AUTHOR]